熒光小體 的英文怎麼說
中文拼音 [yíngguāngxiǎotǐ]
熒光小體
英文
florescence body-
Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1
我們以小鼠為模型,運用組織化學、免疫熒光組織(細胞)化學、流式細胞儀分選方法( facs )以及分子生物學手段,研究了小鼠乳腺的發育規律:小鼠乳腺組織中類乳腺幹細胞:小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定;小鼠類乳腺幹細胞分化的潛能;小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明: 1Using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. it was found that antigens sp - 10 and aeg express on acrosome of all kinds sperms, supporting their roles in sperm - egg fusion. it was firstly described that tmdc - iii express mostly on the equator, neck and some part of tail of human sperm, on the acrosome of mouse and bull sperms, on the acrosome and neck of pig sperm, on the head, neck, and middle piece, principal piece of tail of rabbit sperm
利用間接免疫熒光法對aeg 、 sp - 10 、 tmdc -蛋白在人、小鼠、兔子、豬、牛精子上進行了精確定位, aeg 、 sp - 10在頂體部均有表達,確證了它們與精卵融合有關:首次對tmdc -蛋白在人、小鼠、兔子、豬、牛精子進行了精確定位,它主要存在於人精子尾部主段,小鼠和牛精子頂體,豬精子頂體和頸部,兔精子頭部、頸部和尾部的主段及尾段。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )
本試驗以抗(洛夫林10 ) 、感( 5389 )不同的兩小麥( triticumaestivuml . )品種為材料,採用酶解法制備小麥葉肉原生質體,利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記,並探索了微管骨架標記的影響因素。The amount of light yielded by fluors from the collisions of a β particle is diminishingly small.
熒光體粒子碰撞,發射出的光量很小,而且繼續縮小。The obtained recombinant - 5 - htr plasmid was tranfected into human liver cancer smmc - 7721 cells. all data suggested the expression of plncx - atr could condense cell nucleus and increase nuclear fluorescence intensity, effectively repress the telomerase activity, cell growth and cell proliferation, and induce cell apoptosis
反義重組質粒plncx - atr轉染人肝癌smmc - 7721細胞,結果發現plncx - atr的表達有效地封閉或抑制肝癌細胞的端粒酶活性,使細胞的生長和增殖受到抑制,細胞體積變小、核緻密、核熒光強度增強,且促進其凋亡。The supramolecular complexes of - cyclodextrin cross - linking polymer / l - ( 2 - pyridlazo ) - 2 - naphthol er particles were synthesized by self - assembly. the structure of the supramolecular complex was characterized by the fluorescence analysis, ir spectrometry and chem3d simulation, respectively. the results confirmed that the naphthalene ring of the guest is included in the cavity of - cyclodextrin cross - linking polymer
用熒光分析、 ir 、 xrd及計算機模擬手段對顆粒結構進行了表徵,發現客體分子中極性較小的萘環進入環糊精的疏水內腔,形成了不溶性-環糊精聚合物超分子配合物。The decrease may be due to washing out of fluoroprobe, but this was not an obstacle to its further use ; the quantitative information ( fo i f ) did not change significantly, where f0 and f are the fluorescence intensities in the absence and in the presence of oxygen, respectively
在現場測試中,光纖氧傳感探頭可直接進行溶解氧的原位監測。由於所使用的熒光分子探針的激發光譜在可見區范圍,光源的體積得以減小,達到了實現整體儀器的微型化的目的。In order to overcome problems arisen from the application of x fluorescence analysis into complex spectrum produced by archaeological ceramic fragments with multi - element, low content and thick ground, we have employed the artificial neural network into the research of x fluorescence archaeology and conducted three kinds of research works. as the first one, we have applied the linear olam network ( optimal linear association memory network ) and the non - linear bp network ( back - propagation network ) respectively to analyze the complex x fluorescence spectrum of archaeological samples, and taken both results of spectrum analysis to compare with each other. the second, the method of pattern recognition of bp network was tentatively used to perform intelligent identification of production places of these archaeological samples
針對科技考古中對大量考古陶片進行產地研究時x熒光分析對多元素、低含量、厚基底考古陶片產生的復雜譜分析的問題,將人工神經網路引入x熒光考古中,進行了三方面的研究工作:一是用線性olam網路(最優線性聯想網路)和非線性bp網路(誤差反傳導網路)分別對考古樣品的x熒光復雜譜進行解譜,並比較二者的解譜效果;二是用bp網路模式識別方法對考古樣品的產地進行智能識別;三是為了提高網路運算的可靠性和減小基體效應及電噪聲的干擾和影響,研究並提出了三種網路學習前的譜數據預處理方法。Here we use fluorescently labeled albumin, together with the powerful technique of intraital 2 - photon microscopy to show that renal albumin filtration in non - proteinuric rats is 50 times greater than preiously measured and is followed by rapid endocytosis into proximal tubule cells ( ptcs )
我們使用熒光標記的白蛋白以及強大的活體內雙光子顯微鏡技術,在不伴有蛋白尿的大鼠中發現其腎臟濾過的白蛋白量較以前文獻報道的大50多倍,這些蛋白隨即又迅速被近曲小管細胞以內吞形式攝取。The phase structure of different cu - fe thin films were studied by using grazing incidence x - ray analysis ( gixa ). the texture and residual stress of different cu - fe thin films were measured by scan of x - ray diffraction ( xrd ) and 2 scan with different. the thicknesses of different thin films were characterized by means of small angle x - ray scattering ( saxs ) technique. by using atomic force microscope ( afm ) measured surface roughness of thin films. the component of different thin film was characterized by energy disperse spectrum ( eds ) and x - ray fluorescence ( xrf ). the magnetic properties of cu - fe thin films were measured by means of vibrating sample magnetometer ( vsm ). in addition, the giant magnetoresistance ( gmr ) effects of different films were also measured. the original resistance of the film fabricated by a direction - current magnetron sputtering system is directly affected by bias voltage
利用掠入射x射線分析( gixa )技術對不同cu - fe薄膜的相結構進行了研究;利用xrd掃描及不同角度的2掃描對薄膜進行了結晶織構及殘余應力分析;運用小角x射線散射( saxs )技術測量了薄膜的厚度;採用原子力顯微鏡( afm )觀察了薄膜的表面形貌;運用能量損失譜( eds )及x射線熒光光譜( xrf )對薄膜進行了成分標定;使用振動樣品磁強計測量了不同cu - fe過飽和固溶體薄膜的磁性能;最後利用自製的磁阻性能測試設備測量了真空磁場熱處理前後不同薄膜的巨磁阻值。After bamgal10o17 doped with sr, ca, the cell volume of each doped phosphors is shrunk and the crystal parameter c is decreased
摻雜少量的sr , ca后,所得的熒光粉的晶格參數c變小,晶胞體積也隨之變小。Aqueous fluid volume and [ c1 ~ j were assayed in samples withdrawn by micropipettes. intraocular pressure ( top ), pressure - dependent outflow, and anterior chamber compliance were determined from pressure measurements in response to pulsed and continuous fluid infusions into the anterior chamber using micropipettes. result : in wildtype mice ( gdi genetic background, age 4 - 6 weeks ), iop was 16. 0 ? 0. 4 mmhg, aqueous fluid volume was 7. 2 ? 0. 3 ul, aqueous fluid production was 3. 6 ? 0. 2 ul / hr, aqueous fluid outflow was 0. 36 ? 0. 06 ul / hr / mmhg, and anterior chamber compliance was 0. 036 ? 0. 006 ul / mmhg ( mean ? se, 8 - 10 eyes )
實驗方法包括:將熒光物質用電離子滲透的方法穿透角膜導入活體小鼠的前房中,然後應用共聚焦顯微鏡根據熒光強度變化測量房水生成率;通過顯微注射針吸取房水檢測房水容積和氯離子濃度;顯微玻璃管刺入前房測量眼內壓,並將生理鹽水分別以連續和脈沖兩種方式注入前房,測量房水間隙的順應性和房水排出與眼內壓的相關性。Compared with the traditional ballast, it has the advantage of less power dissipation, less weight, no flicker, no noise and capability of ignition on the low supply voltage
與長期使用的電感式(也叫電磁式)鎮流器比較,電子鎮流器具有節電效果顯著、體積小、重量輕、無閃爍、無噪聲以及在較低的電源電壓下仍然可以啟動熒光燈的優點。( 2 ) compared with control sample, naf treatment can reduce the quantity of both chlorophyll ' s fm and membrance proteins " fem ecited at 278nm and 295nm
( 2 )弱光放置相同時間, naf處理葉片的葉綠素和類囊體膜蛋白內源熒光強度熒光強度均小於參照樣的熒光強度。By studying the luminescence mechanism and the optical spectra, the two reasons for the diminish of light yield are given : the absorption of the fluorescence by the point defects when that produced and the scatter of the fluorescence by the macroscopic defects when that transmitting. in ce : yap scintillators, the available approach to improve the light yield is to diminish the self - absorption of the point defects
通過光譜分析和對發光機制的研究,指出在產生熒光輻射過程時晶體中的點缺陷對熒光的吸收以及熒光收集過程中宏觀缺陷對熒光的散射是造成晶體光產額減小的原因,通過減小晶格缺陷是提高晶體光產額的有效途徑。Nir luminescence due to nd3 + ions from the vtes - derived films was observed, with peak shape similar to that of pure complexes. this indicates the ligands indeed effectively shield nd3 + ions from the chemical microenvironment and giving evidence of the lack of strong chemical interactions with the host matrix when the complex was embedded in the vtes - derived matrix
488nm光激發下,觀察到薄膜中釹的發光,與純配合物粉末熒光相比,峰形變化不大,表明配體對釹起著有效的包裹和保護作一浙江大學碩士學位論文用, nd3 +離子的配位環境受基質的影響很小。Arsenic in a copper concentrate can be rapidly determined by atomic fluorescenc, accompanied with less interference from base copper
摘要用原子熒光光譜法可快速測定銅礦樣中的砷,且基體銅干擾小。Fluorescence body, f body
熒光小體分享友人