熒光抗核抗體 的英文怎麼說
中文拼音 [yíngguāngkànghékàngtǐ]
熒光抗核抗體
英文
fana-
2. to establish a neutrajizing - inhabition time - resolved fluoroimmunoassay ( trfia ) of hepatitis b virus e antibody ( hbeab ) and hepatitis b virus core antibody ( hbcab ) based on the competion. the assay ranges of standard cures were 0 - 16ncu / mland 0 - 8ncu / ml. the within - run coefficient variations for standard samples were less than 10 %. compared this method with radioimmunoassay, the sensitivities were 92. 8 % and 93. 3 %
以中和抑製法建立了乙型肝炎病毒e抗體時間分辨熒光免疫分析法（ hbeab - trfia ）和乙型肝炎病毒核心抗體時間分辨熒光免疫分析法（ hbcab - trfia ） ，標準曲線分析范圍分別為0 - 16ncu ml和0 - 8ncu ml 。. from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體，從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白，將兩者偶聯制備的藻熒光探針，不僅保持了藻膽蛋白很強的抗熒光淬滅能力，而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。In the second trial, this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients. then the effect was identified by wright - giemsa stain, flow cytometry analysis, immunocytochemistry and fluorescence in situ hybridization ( fish ). similary, the 22 % percoll fraction contained mostly haploid cells [ ( 91. 85 ? 5. 18 ) % ] ( p < 0. 005 ) and the mean density in this fraction was ( 1. 010 ? 0. 786 ) x 105 / ml
C法，對15例各種類型不育患者的精液細胞進行分離，並利用瑞姬染色法、流式細胞術、免疫細胞化學和熒光原位雜交oisffi等方法，從細胞形態特徵、 dna倍體、細胞表面標i己與分化抗原，以及原位雜交信號的數目和位置結合細胞核特有的形態等方面加以鑒定。In hela cells, by using anti - brgl antibodies, anti - rna polymerase ii antibodies and anti - nfl / ctf antibodies, the core subunit brg1 of baf complex and rna polymerase large subunit were found well immunofluorescently co - localized, while nf1 / ctf and rna polymerase large subunit were poorly co - localized. brg1 and nf1 / ctf were also well co - localized. in order to further reveal the relationships of baf complex with nf1 / ctf and rna polymerase ii large subunit at the ultra - microscopic level, we performed the double - labeling immunoelectron microscopy experiment with hela cells
以hela細胞為材料，分別用抗brg1抗體、抗rna聚合酶抗體和抗nf1 ctf抗體進行免疫熒光共定位分析，發現baf復合物的核心亞基brg1和rna聚合酶的大亞基存在很好的熒光共定位現象， nf1 ctf和rna聚合酶的大亞基之間的共定位現象不明顯， brg1和nf1 ctf也有很好的共定位現象。分享友人