熒光探測法 的英文怎麼說
中文拼音 [yíngguāngtàncèfǎ]
熒光探測法
英文
fluorescent penetrant method- 熒 : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 探 : Ⅰ動詞1 (試圖發現) try to find out; explore; sound 2 (看望) call on; visit; see 3 (向前伸出)...
- 測 : 動詞1. (測量) survey; fathom; measure 2. (測度; 推測) conjecture; infer
- 法 : Ⅰ名詞1 (由國家制定或認可的行為規則的總稱) law 2 (方法; 方式) way; method; mode; means 3 (標...
- 探測 : survey; search; sound; probe; sounding; detection; acquisition; reconnaissance; localization; fin...
-
The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment
目的本課題旨在探索一種新的str基因座復合擴增方法,我們稱為嵌合引物str復合擴增法。應用熒光標記毛細管電泳激光自動檢測技術平臺,建立一套新的法醫str基因座復合擴增體系,並按照美國dna分析方法技術工作組( thetechnologyworkinggroupondnaanalysismethods , twgdam )的指導方案進行法醫學實用性研究。After study on the technology of probe head of the optical fiber, a biosensor for determination of cholesterol which based on fluorescence quenching and adopted phase shift & phase lock technique to detect the change of light intensity was developed, in this biosensor, the cellulose acetate cod enzyme membrane was took as sensitivity basic dollar, ru ( phen ) 32 + was took as indicator of oxygen and furcated optical fiber as conduct carrier of light signal
通過對光纖探頭組裝技術的研究,以醋酸纖維素cod酶膜為敏感基元,釕( ) -鄰菲咯啉為氧指示劑,分叉光纖為光信號傳導載體,採用相移法和鎖相放大技術設計了一種基於熒光猝滅原理的測定膽固醇用的生物傳感裝置。7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團,以tarma標記探針3 』端為淬滅基團,以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a )為檢狽目標,設計、篩選出特異性引物和探針,優化實驗參數,建立了轉基因植物通用性熒光pcr定性檢測方法體系。On the basis of the mentioned above and the real - time pcr principal, we have designed and optimized the primers and probes, set up the real - time qualitative pcr detection system. meanwhile, using the endogenous zein - maize and lectin - soybean as interior collate gene, epsps and cryia ( b ) as target genes, set up the real - time quantitative pcr detection system
在此基礎上,根據熒光pcr原理,設計、優化引物和熒光探針,建立了熒光pcr定性檢測方法體系,並以玉米內源基因( zein ) 、大豆內源基因( lectin )作為內標基因,以epsps和cryia ( b )作為目的基因,建立了熒光pcr定量檢測方法體系。The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials, from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption, elucidating their binding affinity, fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy. with the help of the concerned the data, the analysis was done to prove whether it can be applied to the zinc detection, in other words, whether it can be used as a new fluorescence probe for zinc detection
本實驗首次選用在生物體內與zn ~ ( 2 + )鍵合能力很突出的物質? ?組氨酸和半胱氨酸,採用類似於多肽合成的方法,在其羧基或氨基分別嫁接上一個帶有標記的基團,生成穩定的共價鍵化合物;在此化合物中模擬生理濃度條件加入鋅離子,通過紅外圖譜、紫外圖譜或熒光圖譜的變化分析鋅離子對標記基團是否產生影響,再結合有關數據分析其是否適合檢測鋅離子,即是否可能作為新的鋅離子熒光探針。The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software
方法根據mtdna控制區及其周圍區域的序列,設計多對引物,探索優化擴增體系,使擴增條件能夠同時滿足多對引物的需要,用sanger末端終止法及熒光標記技術對樣本進行dna測序, sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。In this thesis the in situ brilliant cresyl blue ( bcb ) dimer with weak fluorescence in the solution of anionic surfactant ( as ) sodium lauryl sulfonate ( sls ) was used as fluorescence probe. at the same time, the new method for the determination of bovine serum albumin ( bsa ) was built. the linear range was 0 - 7mg / l and the detection limit ( 3 o / k ) was 3. 66 10 - 3 mg / l, the r. s. d for the determination of 2. 00mg / l bsa was 2. 77 %
本論文以陽離子染料燦爛甲酚藍在陰離子表面活性劑十二烷基磺酸鈉存在下形成的現場弱熒光二聚體為熒光探針,建立了測定牛血清白蛋白的新方法,該法線性范圍為0 7mg / l ,檢測限為3 . 66 10 ~ ( - 3 ) mg / l ,對2 . 00mg / l幾的牛血清白蛋白平行測定8次,相對標準偏差為2 . 77 ,說明本法準確可靠。This thesis consists of four chapters : ( 1 ) a review ; ( 2 ) study on the optical chemical membrane for detection of lead ion by using 2 - hydroxy - 1 - naphthaldehydene - 8 - aminoquinoline as fluorescence indicator ; ( 3 ) study on the optical chemical membrane for detection of lead ion by using meso - ( 4 - methyloxyphenyl ) porphyrin as fluorescence indicator ; ( 4 ) study on the optical chemical membrane for detection of lead ion by using 2 - methene - benzimidazole monoaza - 18 - crown - 6 as fluorescence indicator. in chapter one, the laboratory method for determination of lead ion, the progresses of chemical sensor for detection of lead ion recently and the introduction of the basic theory, relative merits of sol - gel and its application in optical chemical sensor were involved
第二章利用有機改性的溶膠-凝膠包埋方式,以2 -羥基- 1 -萘甲醛- 8 -氨基喹啉為熒光指示探針,結合正交實驗方法探索了幾個重要因素,如加水量、醇硅比、催化劑、添加劑等對成膜性的影響,制備了對水中鉛離子有較好響應的傳感膜,其線性范圍為1 . 0 10 - 7 ~ 5 . 0 10 - 5mol / l ,檢測限為3 . 6 10 - 8mol / l 。Abstract : this article briefly discusses how to develop the radiated inspecting system used in detecting the illicit medicine and the sensitive materials, detecsts the principles, and discusses the problem that the nucleus resonance fluorescence way is a kind of chred sensitive survaying way
文摘:主要是探索如何研製用於違禁藥物以及敏感材料的探測系統,討論了探測原理,論述了核共振熒光法是一種高敏感度的測量方法The field surveys and lab experiments tvere designed to study the distribution and source of pah " poliution in x " ater, sediment ; the pah " concentration and distribution in marine fish organ ( of the fish lateolabimjapo ) 1iclls and pagivsonills inajor ) ; the pah " bcf factors of liver and bile in marine fish ( laleolabral jtwo ) 1itits and pag7vso ) ) ills ) ) 1ajbr ) ; the reiationship between pah " exposure and biochemicai response ( cat sod, lpo ) and the metabolic rate of naphthalene and pyrene in fish bile
4優化了魚膽汁中1 ?羥基萘和1 ?羥基芘的熒光分析方法,測定了1 ?羥基萘和1 ?羥基芘在鱸魚、真鯛膽汁中的含量,探討了萘和芘在魚膽汁中的代謝比率、清除作用。結果顯示: ( 1 )膽汁中萘的代謝比率低,易蓄積;而芘的代謝比率高,易清除。First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods
實驗選用表面平整的德國玻片,將清洗好的玻片分別進行羥基化、氨基化、醛基化,採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量,研究兩種檢測方法之間的內在聯系,從而確定表徵玻片表面寡核苷酸探針固定率的方法。So the method was accurate and reliable. the in situ nile blue ( nb ) dimer with weak fluorescence in the solution of anionic surfactant sodium dodecylbenzene sulfonate - 6 ( dbss ) was also used as the fluorescence probe for the determination of total proteins in human serum
以陽離子染料耐爾藍在陰離子表面活性劑十二烷基苯磺酸鈉存在下形成的現場弱熒光二聚體作為測定人血清總蛋白的熒光探針。該法用於實際樣品的測定,與臨床方法測定的結果相吻合。The main sources of the measurement uncertainty by xrf are analysed and some factors impacting on the uncertainty are discussed, which is good for developing the measurement uncertainty in the actual work
著重分析了採用x射線熒光能譜法測定貴金屬含量時測量結果不確定度的主要來源,並探討了幾種因素對不確定度的影響,有利於在實際檢測中對其進行合理的評定工作。In this thesis, the energy transfer system of acridine orange ( ao ) - rhodamine b ( rb ) dimer was built for the determination of dna as fluorescence probe
本論文以吖啶橙?羅丹明b二聚體能量轉移體系作為熒光探針,建立了測定dna的新方法。The quantitative determination of protein and dna is a common analysis contents in biology chemistry and life science. so it is very important to find fluorescence probe with high sensitivity and low detection limit
對蛋白質和dna進行定量測定是生物化學和生命科學中經常涉及的分析內容,建立高靈敏度、低檢測限的熒光探針定量測定的方法仍具有重要意義。Petroleum products. determination of sulfur in petroleum products. combustion and detection by ultraviolet fluorescence
石油產品.石油產品中硫的測定.燃燒法和紫外線熒光探測Some new fluorescent probes and sensors are prepared using these techniques, and they are utilized to determine some medicines and toxic materials in the environments
利用這些方法制備了幾種新型熒光探針及傳感器,並用於藥物和環境毒物的檢測中。In this dissertation, a research of grazing x - ray fluorescence analysis has been carried out, and on this basis, an energy - dispersive method to measure soft x - ray fluorescence based on grazing emission has been studied
本文在分析國內外掠射x射線熒光分析技術的基礎上,對能量色散掠出射軟x射線熒光探測方法進行了深入的研究。A novel real - time fluorescent rt - pcr was established to detect pnrsv this technique uses a taqman probe to monitor in real time amplification of target genes, a taqman probe labeled by a quenching and fluorecenting was added to the pcr reaction buffer
另外還設計了taqman熒光探針,確定了檢測條件和參數,建立了實時熒光rtwcr方法,該方法是指在pcr反應體系中加入帶有熒光基團互補探針,如果有pcr反應(擴增) ,熒光信號就較大。Detector of organic water pollutants organic water pollutants like sewage and landfill can be detected using fluorescence a natural phenomena where certain substances absorb and emit light
利用熒光的方法,可以探測出包括下水道污水和進行垃圾填埋造成的有機水污染。而熒光則是利用某種特殊物質吸收並發出光線的自然現象。分享友人