熒光素標記的 的英文怎麼說
中文拼音 [yíngguāngsùbiāojìde]
熒光素標記的
英文
fluorescein-labeled- 熒 : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 素 : Ⅰ形容詞1 (本色; 白色) white 2 (顏色單純) plain; simple; quiet 3 (本來的; 原有的) native Ⅱ名...
- 標 : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
- 記 : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
- 的 : 4次方是 The fourth power of 2 is direction
- 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
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Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。Although this method gives high sensitivity, the radioactive labels present many problems such as a potential hazard to analyst and environment, which limited its application in dna diagnostic laboratories. in order to overcome these problems a serious of non - radioactive dna probes such as fluorescent, chemiluminescent and electrochemical probes have been developed. although these new methods display many advantages, they have not been used to take place completely the traditional method because of low sensitivity or complex equipment or other shortcomings
自20世紀80年代以來,各種非同位素如酶、熒光素、生物素、地高辛標記的化學發光法和熒光分析法以及以電活性物質做標記的電化學方法相繼問世,這些方法雖然在一定程度上克服了同位素標記的缺陷,但由於存在靈敏度不夠高或檢測系統龐雜或儀器價格昂貴或標記物不穩定等缺陷,還不能完全取代傳統方法。The results show that the porous materials possess three - dimensionally ordered structure and the fitc labeled bsa materials exhibit good fluorescence emission ability
表明所制備的孔材料具有規整的三維有序孔結構,熒光素標記的牛血清白蛋白三維有序大孔材料具有良好的熒光發射性質。Three dimensionally ( 3d ) - ordered macroporous materials of bovine serum albumin ( bsa ) and fluorescein isothiocyanate ( fitc ) labeled bsa were prepared by means of colloidal crystal as template
摘要用膠體晶體為模板制備了牛血清白蛋白和異硫氰基熒光素標記的牛血清白蛋白的三維有序大孔材料。The dendrimers would carry one of the stable isotopic or fluorescent labels to identify the presence or absence of a protein that can be further deeloped for use as a disease indicator, or biomarker
攜帶有穩態同位素或熒光標記的樹狀高分子可以鑒別標本中是否存在某種蛋白質,從而可以將其作為某種疾病的化學指標或生物標記。In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )
本試驗以抗(洛夫林10 ) 、感( 5389 )不同的兩小麥( triticumaestivuml . )品種為材料,採用酶解法制備小麥葉肉原生質體,利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記,並探索了微管骨架標記的影響因素。The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
本文回顧了李壞死環斑病毒的檢測方法,較全面地評述了國內外病原物檢測技術研究進展:在模板的擴增有各種pcr技術、 nasba技術;在信號的放大有同位素、生物素或dig標記的cdna和crna探針,分支探針和肽核酸探針;模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術;模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用,本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ,並將提純的人精子甘露糖受體( purifiedmannosereceptor , pmr )作用於去透明帶的金黃地鼠卵母細胞,運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素( tritc - lca )免疫熒光標記技術觀察pmr對卵子的影響。Application of quantum dots labeling to the interaction between reconstructed - phage display peptide and insulin receptor
量子點熒光標記在重組噬菌體表面展示肽與胰島素受體相互作用中的應用分享友人