熱穩定蛋白 的英文怎麼說

中文拼音 [rèwěndìngdànbái]
熱穩定蛋白 英文
heat-stable protein
  • 穩 : 形容詞1 （穩定; 穩當） steady; stable; firm 2 （穩重） steady; staid; sedate 3 （穩妥） sure; rel...
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 穩定 : 1 （使穩定） stabilize; steady 2 （穩固安定） stable; steady 3 （物質的性能不易改變的作用） stabi...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The metabolism of these extreme microbes during the production of maotai liquor would further produce multiple enzymes of thermal stability such as amylase, protease, saccharifying enzyme, cellulose, glucase, xylanase, and each kind of dehydrase involved in redox reaction, and dna polyase etc

    茅臺酒釀造過程中極端釀酒微生物代謝產生多種熱穩定性的酶，如澱粉酶、蛋白酶、糖化酶、纖維素酶、葡萄糖甘酶、木聚糖酶、參與氧化還原反應的各種脫氮酶、磷酸烯醇丙酮酸激酶及dna聚合酶等。
  3. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  4. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  5. The results showed that thermal and storage stabilities of the immobilized papain were higher than those of the freely soluble one, and that papain directly was immobilized onto the microspheres " surface without any spare space provided a higher stability

    結果表明:由於木瓜蛋白酶直接固定於微球表面而沒有剩餘空間，這給固定化酶提供了更高的穩定性，所以固定化木瓜蛋白酶在熱穩定性與儲藏穩定性方面優于游離酶。
  6. In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures

    幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。
  7. The micro particle acidic proteinase was studied respectively in respects of its heat stability and storage stability, when 4 % of it was added in a pre - mixed pig feed

    摘要分別從耐熱性及在4 %豬用預混料中的貯存穩定性兩個方面，對微丸型酸性蛋白酶進行了研究。
  8. The problem of the protein stability changed by solvent environment is in the limelight

    蛋白質的穩定性隨溶液環境的變化一直是人們關心的熱點問題。
  9. However there were little changes in the level of heat - stable proteins

    但是馴化過程中，熱穩定蛋白水平並無明顯變化。
  10. Calmodulin ( cam ) is a small heat - stable ca2 + - bindinding protein highly conserved in eukaryotes

    鈣調素（ calmodulin ， cam ）是真核生物中高度保守的一種小的熱穩定性鈣離子結合蛋白。
  11. I. roles of dehydrins in plant tolerance to freezing and drought. accumulations of heat - stable proteins and dehydrins induced by dehydration, low temperature, osmotic stress, high salt or aba treatment were investigated in protocorm - like - bodies ( plbs ) ofdendrobium candidum. moreover, we described the relationship between these accumulations and the development of tolerance to stresses

    一、脫水蛋白對植物抗凍性和抗旱性作用研究以鐵皮石斛類原球莖（ plbs ）為材料，研究脫水、低溫、高滲、高鹽和外源aba處理與誘導熱穩定蛋白和脫水蛋白的關系，以及脅迫誘導蛋白的積累與各種抗逆性形成的相關性。
  12. Some important potential advantages of producing recombinant proteins in plants for vaccine development include : production as well as storage can occur near the site of use ; the heat stable formulations eliminate the need for refrigeration ; and the need for hypodermic delivery ( needles ) can be also eliminated

    利用植物來生產重組蛋白進而用於疫苗開發的其他重要的潛在優勢還包括：生產過程和儲存一樣可以發生在需要的地點附近；熱穩定的成分消除了冷凍的需要；同時可以避免用針進行皮下注射。
  13. However, nacl treatment did not affect the level of heat - stable proteins

    而nacl處理對熱穩定蛋白影響不明顯。
  14. Heat - stable protein of 17. 0 kda molecular mass was induced to accumulate by peg treatment, whose level was increased with the rise of peg concentration

    Peg處理誘導17 . 0kda熱穩定蛋白的積累，其含量隨peg濃度的增加而增加。
  15. Interestingly, exogenous aba treatment of plbs before desiccation could also induce the accumulation of soluble sugars, heat - stable proteins and dehydrins

    外源aba的預處理可明顯提高可溶性糖、熱穩定蛋白和脫水蛋白的含量。
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