片段化 的英文怎麼說
中文拼音 [piānduànhuà]
片段化
英文
gragmentation-
Cloning and sequencing of acc oxidase gene from sugarcane
氧化酶基因片段的克隆與序列分析Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Third, it is this " moment " of the segment of space - time which based on the the philosophy of the nature and describing the mechanical movement that symbolize the transform from the view of integralization in classical atomism of mathematics to the view of integralization of modern real number
另外，正是基於新的自然哲學的、作為描述機械運動的時空片段的「瞬」標志著古典數學原子論的積分觀向現代的分割實數連續統的積分觀的轉化，而現代數學的微分概念更是直接源於描述機械運動的速度和與運動軌跡密切相關的曲線的切線問題。Gene cloning provides a means of purifying and propagating specific dna segments.
基因克隆化提供了一種純化和擴增特定DNA片段的方法。Two captive populations could n ' t be defined as separate evolutionary significant units ( esus ) because of lacking of genetic divergence between them, and should be considered as a single esu in the conservation of the species. by comparing the sequences of control region of mitochondrial dna from three species of crocodiles, it is revealed that the smallest genetic diversity exists between alligator sinensis and alligator mississipiensis. a portion of mitochondrial nd4 and cytochrome b gene of 3 species of crocodilian was sequenced
百年來關于揚子鱷的分類地位存在著很多爭議，本文利用測得的揚子鱷（ alligatorsinensis ） 、暹羅鱷（ crocodlylussiamensis ）和灣鱷（ crocodylusporosus ）的mtdnand4和cytb基因序列，以及從genbank中獲得密西西比鱷、凱門鱷和海龜（ cheloniamydas ）的nd4基因和cytb基因相應片段，構建以海龜為外群的系統進化樹。Confocal microscopy observation followed immune - fluorescence staining with anti - gp130 showed that gp130 could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus
為了進一步證實上述發現，我們表達並純化了gst - gp130胞漿區融合蛋白和gst - tle1獨特性片段融合蛋白，並制備了特異性抗tle1多克隆抗體。Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control
選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為pThis time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting
本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding pcr product from brussel sprouts, kohlrabi and kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon
對各pcr產物分別進行回收、純化、載體連接和序列測定及基因結構分析等，結果表明，該片段在抱子甘藍、羽衣甘藍和球莖甘藍三種作物中的全長分別為1665bp 、 1650bp和1650bp ，包括相應基因的前兩個外顯子和內含子以及第三個外顯子的22bp序列。Five analogues and five segments were designed and synthesized by using solid phase synthesis method according to separated papaver somniferum pollen tridecapeptide with antitumor activities as leading peptide. their primary secondary structures in solution were determined by cd spectra and their inhibitive activities to human liver and mammary gland cancer cells were assayed by mtt method. the relationship of structure - activity was studied and discussed
罌粟花粉十三肽對人肝癌和人乳腺癌腫瘤細胞具有明顯的抑制作用，以其為先導化合物，設計併合成了5個類似物和5個片段，結合cd譜測定的二級結構及它們對人肝癌和人乳腺癌腫瘤細胞的抑制作用測定結果，研究並討論了該肽結構與抗腫瘤活性的關系Healthy rabbits were inoculated each with vaccine of the recombinant fused pili - el - 2 protein at 250ul per dose to potentiate the immunogenicity. rabbits inoculated two times at one week interval
擴增此質粒，回收bamh切出的2 . 5kb的基因片段，與pme290 （ bamh酶切後去磷酸化）載體連接。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。Compared with the 5. 8s complete sequence of the snail arion rufus, its1 and its2 regions were recognized and combined for analysis. from sequence observation, it showed that the zhejiang sample has more inserted sites and fragments while the sequences of other three are nearly all the same. the average g % + c % of the four individuals was 46. 8 % while the zhejiang sample ' s was 48. 3 % and the other three ' s were all about 46. 2 % ; ts / tv and genetic distance mainly lies between the zhejiang sample and the other three individuals, which were 0. 8 and 0. 07 respectively
用於比較的序列長約350bp ，觀測一級結構，加拿大、墨西哥灣扇貝和美國二代個體的its1和its2序列幾乎完全相同，而浙江個體則具有較多的插入位點與片段； 4個個體平均g + c含量46 . 8 % ，其中浙江個體為48 . 3 % ，其它3個個體均為46 . 2 %左右；轉換顛換比與遺傳距離主要存在於浙江個體與其它3個個體之間，分別為0 . 8和0 . 07左右；以櫛孔扇貝作外群構建的分子系統樹表明：浙江群體已產生了一定的分化。In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not
合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。The cumulus expansion, cumulus cells dna fragmentation, oocyte meiotic maturation and degeneration were determined 44 h after incubation. snp inhibited cumulus expansion and cumulus cells dna fragmentation in a dose - dependent manner
結果發現， snp可劑量依賴性地抑制卵丘細胞的擴展和dna片段化，同時抑制ceos中卵母細胞的減數分裂恢復，抑制m向m期轉變。C. chinensis extract prevents the neuronal differentiated pc 12 cells apoptosis, dna fragmentation, and enhances the cells viability at concentration up to 20 mg / l in serum - deprivation condition
菟絲子提取物（ 0 - 20mg l ）能劑量依賴地抑制去血清引起的細胞凋亡，防止基因組dna片段化，提高成活細胞的比例。Based on these results, the strategies for conservation e. mollis population to prevent the isolation and fragment of the populations and maintain the high genetic variation among the populations. moreover, it should be avoid the decline of gene flow was resulted from the isolation of the populations so that resulted in the differentiation and genetic decline of the population. finally, it should be adopt some method to spread continuous distribution and extend genetic diversity of the population
針對翅果油樹的遺傳結構和遺傳多樣性的研究結果，提出了保護翅果油樹種群的策略：維持現有翅果油樹種群高的種內遺傳變異水平，遏制由於人為原因而導致的翅果油樹種群隔離或片段化的趨勢，防止由於種群隔離造成基因流降低，從而引起種群分化和種群的遺傳衰退，並採取適當措施擴大種群的連續性和種群內的遺傳多樣性水平。The essence of global shift is " fragmentization " and geographic re - location of value chain by multinationals
國際製造業轉移實質是跨國公司價值鏈「片段化」和空間重置的過程。分享友人