特異性中毒 的英文怎麼說
中文拼音 [tèyìxìngzhōngdú]
特異性中毒
英文
idiosyncratic intoxication- 特 : Ⅰ形容詞(特殊; 超出一般) particular; special; exceptional; unusual Ⅱ副詞1 (特別) especially; v...
- 異 : 形容詞1 (有分別; 不相同) different 2 (奇異; 特別) strange; unusual; extraordinary 3 (另外的;...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 特異性 : distinction
- 特異 : 1 (特別優異) exceptionally good; excellent; superfine2 (特殊) peculiar; distinctive特異功能 s...
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Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。Abstract : this paper describes the role of the sialylsugar chains on cell surface in the attacking process of the influenza virus, and also binding specificity of hemagglutinin of the influenza virus to the sialylsugar chains
文摘:介紹了細胞外表面上含唾液酸的糖鏈在流感病毒侵襲細胞中的作用以及糖鏈與流感病毒表面糖蛋白-血凝素結合的特異性。A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以豬瘟兔化弱毒犢牛睪丸細胞毒( hclv )中提取的細胞總rna為模板,以rt - pcr技術,擴增出了完整e2基因的cdna片段。經電泳、酶切分析,證實了所擴增片段為e2基因特異性片段。However, only a few host factors with clear in vivo function have been identified. by using pcr and 5 " and 3 " race, we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene, tom1, which supports the replication of tobacco mosaic virus
根據從擬南芥中已經克隆的支持煙草花葉病毒復制的基因tom1序列設計一對特異性引物,用rt - pcr的方法獲得番茄同源基因的部分序列,然後利用5 』 race與3 』 race方法從番茄中克隆出全長ttom1基因。The combined sampling pcr detection method was found to be fully feasible for the rapid ( approximately 2. 5 h ) and highly specific ( no cross - reactivity ) identification of the labile airborne virus in the air containing elevated concentrations of other microorganisms
使用即時定量聚合酵素反應技術結合個人采樣器採集空氣中病毒,證明於空氣中含有其他微生物之情形下本技術對偵測特定病毒之所需分析時間相當短且特異性相當高。In contrast, most mature somatic cells in human tissues express undetectable or low telomerase activity, implying the existence of a stringent and negative regulatory mechanism. in this study, we report the effects of cisplatin on telomerase activity in human ovarian cancer cells. the special relationship between activity and cancer has a important application value
順鉑( ddp )是卵巢癌化療中最有效的基礎藥物之一,它是一種類烷化劑的抗腫瘤藥物,為細胞周期非特異性藥物,可作用細胞周期的任一時相,利於臨床的聯合用藥,但由於ddp的諸多毒副作用及卵巢癌細胞易產生對其耐藥性,常使化療效果不滿意。The neutralization test is based on the neutralization of virus by specific antibodies found in the serum.
中和試驗是基於病毒可被存在於血清中的特異性抗體。The two isolates were positive reaction hi polymerase chain reactions with two pair of primers specific for alv - j and gave antigenically strong reaction hi the indirect fluorescence antibody assay ( ifa ) with alv - j specific monoclonal antibody je9. negatively - stained electron microscopic and immune - electron microscopic observation demonstrated that viral particles of the inner mongolia and shandong isolate of alv - j, respectively designated imc10200 and sdc2000 strain of alv - j, showed characteristic morphology of alv
利用pcr和間接免疫熒光反應進行鑒定,兩株j亞群禽白血病病毒可以被兩對alv - j特異性引物擴增(特異條帶約2 . 2kb和545bp ) ,且在特異性單克隆抗體je9的間接免疫熒光檢測中呈現強陽性熒光反應。The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達: pcr擴增出當前豬瘟流行野毒株,中國豬瘟兔化弱毒( c株)兔脾組織毒ez基因的主要抗原區,將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達,經sds page檢測表明,重組質粒能表達ez基因主要區蛋白, westernblot檢測表明,誘導表達蛋白與豬瘟陽性血清發生特異性反應,表達量為35和38 ,可用於基因工程診斷抗原。It is chloride - dependent and all the abnormalities can be corrected by thiazide diuretics which is the specific antagonist of the na - cl cotransporter ( ncct ). all these indicate that the disease should be relative to the ion transportation
它以高血壓,高血鉀和代謝性酸中毒為主要臨床表現,它是氯依賴性的,臨床異常能夠被鈉-氯共轉子ncct的特異性拮抗劑? ?雙氫克尿噻糾正,說明此疾病的發生和離子轉運相關。The results of the first part showed that both cd55 and cd59 neutralizing antibody can enhance the lysis or neutralization activity of complement. however, since rca are distributed widely, techniques that can mediate target rca blocking on virions are necessary
最後,病毒中和功能實驗結果顯示,我們制備的雙特異性抗體具有良好的誘導補體活化,中和eev病毒感染的作用,與親本抗體相比有增強的eev病毒中和活性。The ns5 protein of dengue virus is the largest molecule encoded by the virus genome with a molecule weight of 104 000. recent research work indicates that the ns5 protein acts as the rna - dependent rna polymerase ( rdrp ) in virus which has the ability to recognize and bind its template rna to synthesize a complementary strand
近年來的研究表明, ns5蛋白具有rna依賴的rna聚合酶( rdrp )功能,即可以識別具有相對特異性的模板rna並與之結合,合成與模板互補的rna鏈,在病毒基因組復制過程中起關鍵作用。But it is doubt that the speciality and stability for the outcome of detecting bdv orf i genome. we will detect the indviduals ' ( include the neuropsychiatric 4 patients with bdv orfii postive or not and healthy blood donator ) middle fragment of bdv orf i to help defmiting whether internal healthy people and the certain neuropsychiatric patient such as with viral encephalitis, multiple, schizophrenia, affective disorder have catch a bdv infection or not, most of all, the research is for understanding if there is any difference in the fragment from animal and human beings infected with bdv, thereby to find another sensitive and stably marker for confirming bdv infection, and establish a solide bottom for advanced study
但對另一編碼p40蛋白序列的檢測結果的特異性和穩定性還不肯定,我們通過對先前檢測bdvorf基因片段為陽性或陰性的神經精神病人和健康獻血者進行了bdvorf基因中部片段的檢測。以幫助確定在國內健康人群和某些神經精神疾病患者中如:病毒性腦炎、多發性硬化、精神分裂癥以及情感障礙等疾病中是否存在bdv的感染,更為重要的是了解感染人和動物的該病毒片段是否存在差異,從而找出另一檢測bdv感染的敏感性和穩定性的標記物,為下一步的研究奠定良好的基礎。Classical swine fever ( csf ) is one of major contagious diseases in pig breeding industry. in order to gain massive diagnostic antigen csfv, and large scale of csfv specific major protective antigen protein ( e2 envelope glycoprotein ) in eucaryotic cells, which are prepared into subunit vaccine for effective prevention against csf
豬瘟是危害養豬業的主要傳染病,為了得到大量的豬瘟病毒特異性診斷抗原,並試圖在真核細胞中大量表達豬瘟病毒特有的免疫保護性抗原,即e2囊膜糖蛋白,為進一步制備豬瘟亞單位疫苗有效預防本病奠定基礎。In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules
研究發現呷基因的調控區存在多種轉錄因子結合位點,功能主要涉及組織特異性表達調控,細胞生長發育相關基因的表達調控,刺激反應基因的表達調控等; ndrgz蛋白在結構上屬于a小水解酶類折疊,折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似,提示ndrgz蛋白具有一定酶活性,可能參與細胞抗氧化應激反應,維持細, an ) armtbffiofbfochmilsyn ) mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡,參與內外源有毒物質的代謝等。The hemaglutinin ( ha ) of aiv plays the key role in determing the pathogenicity, cell receptor binding property and host range of the virus. the homology of the ha sequences reported and registered in genbank of different strains of h _ ( 5 ). h _ ( 9 ) subtype was respectively analyzed and compared with each other. the conservative domin of ha seqence of h _ ( 5 ), h _ ( 9 ) subtype was selected for pcr amplification. two sets of specific primers were designed
本研究根據genbank中查出的禽流感病毒h _ 5亞型,禽流感病毒h _ 9亞型的ha片段基因組序列,利用dnasis軟體分別對aiv的h _ 5 、 h _ 9亞型ha基因區域進行同源性比較,設計篩選出兩對聯合pcr反應的特異性引物,其中一對是h _ 9亞型通用引物,擴增出ha片段695bp ,另一對引物為h _ 5亞型的通用引物,擴增出的ha區域部分目的片段約為448bp 。Results only 76 people with blood antibody titer > 1 : 5 had the cellular immune effect ( the particular plt was marc than 5 % ), 35 people without antibody and 4 people whose blood antibody titer was 1 : 5, nobody had the cellular immune effect
結果狂犬病疫苗接種后,抗體效價達到大於1 : 5的100人中, 76人同時產生細胞免疫力(即狂犬病毒特異性淋巴細胞轉化率大於5 % ) ;而抗體效價等於1 : 5的4人和未產生抗體的35人中,無人產生細胞免疫力。The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses, specific primers were designed and synthesized. from the serum of two chinese patients with non - a - e hepatitis, one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr, another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too. from one blood donor serum, two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively
Sen病毒d和h亞型中國分離株的克隆及序列分析我們在前期工作的基礎上,結合已發表的文章及基因序列,針對senv - d和senv - h基因組設計特異性引物,利用套式pcr技術從兩例non - a - e肝炎患者血清中分段克隆得到了一個senv - d亞型分離株( senv - d - bj1 )的全部編碼區基因序列,還得到了一個senv - d亞型分離株( senv - d - bj2 )的大部分編碼區基因序列(包括orf1和orf2 ) ;從一例健康人血清中分段克隆得到了兩個senv - h亞型分離株( senv - h12 - 1和senv - h12 - 2 )的全部編碼區基因序列。The use of transgenic plants for producing fmdv vp1 antigen protein has been reported and may prove to be safer than conventional vaccines. recently, fmdv vp1 protein had be expressed in transgenic plants
最近利用轉基因植物表達的口蹄疫病毒vp1蛋白注射和飼喂小鼠后,能夠誘導其體內產生特異性中和抗體,並使小鼠得到有效的免疫保護。分享友人