特異質反應 的英文怎麼說

中文拼音 [tèyìzhífǎnyīng]
特異質反應 英文
idiosyncratic reaction
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 反 : Ⅰ名詞1 （方向相背） reverse side 2 （造反） rebellion 3 （指反革命、反動派） counterrevolutionari...
  • 應 : 應動詞1 （回答） answer; respond to; echo 2 （滿足要求） comply with; grant 3 （順應; 適應） suit...
  • 特異 : 1 （特別優異） exceptionally good; excellent; superfine2 （特殊） peculiar; distinctive特異功能 s...
  1. However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting

    利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。
  2. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  3. Perovskite - type complex oxides of la1 - xsrxga1 - ymgyo3 compositions are characteristic of superior intermediate temperature oxide - ion conductivity and chemical stability, which are expected to be used in solid oxide fuel cells, sensors, electrocatalysis, membrane separators and membrane reactors. they have highly promising prospects in energy, metallurgy, chemical engineering and environmental protection, etc. in this dissertation, improved embeded sintering method has been employed to prepare la0. 8sr0. 2ga1 - ymgyo3 ( lsgm ) electrolyte, and the contributing factors to preparation, microstructure characteristics, conduction properties and thermology properties of lsgm electrolyte have been investigated

    鈣鈦礦結構la _ （ 1 - x ） sr _ xga _ （ 1 - y ） mg _ yo _ 3復合氧化物具有優異的中溫離子導電特性和化學穩定性，作為固體電解質可用於固體氧化物燃料電池、傳感器、電催化、膜分離和膜反應器等，在能源、冶金、化工、環保等領域具有廣泛的應用前景。
  4. By virtue of the anhydride property of the isoimide rings, the piis were reacted with the alcohols to form poly ( amic acid ) esters ( paes )

    利用異酰亞胺環具有酸酐性質的特點，使之與醇類反應，得到溶解性能優良的聚酰胺酯（ paes ） 。
  5. Depending on their level of mauriy, dcs express proininnt 1evels of mhc class l and class ii molecules, as well as costdrilatory molecules such as cd40, cd80, and cd86. wl studies show them to be responsible pranarily for sensitto naive t cells in their first empsure to anigen

    但是，近年來的研究表明， dc是一類異質性細胞群體，具有不同的亞群和不同的功能狀態，不但在增強免疫反應上具有特別重要的作用，也具有誘導特異性的免疫耐受的作用。
  6. When the antigen ( brief named ag ) invaded into the organism, and the body can produce a kind of material ( high molecular weight protein, named antibody, brief named ab ) that can distinguish this antigen and evacuate it, the antigen and antibody take place the immuno - reaction. using their highly sensitivity to response mass change and specificity, it can be fabricated a piezoelectric immunnosensors

    當異種蛋白（稱為抗原， antigen ，簡稱ag ）侵入生物體內時，體內能產生識別此類異物並將其排出的物質（大分子量蛋白質，稱抗體antibody ，簡稱ab ） ，抗原與抗體發生免疫反應，利用抗體（或抗原）對抗原（或抗體）的特異性識別功能和壓電晶體的高靈敏質量響應可製成壓電免疫傳感器。
  7. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  8. Based on laser molecular beam epitaxy, the strain behavior and the corresponding control technology in oxides heteroepitaxial system, especially in ferroelectric thin films with perovskite structure, was systematically studied by using in situ reflected - high - energy - electron - diffraction ( rheed ). some original and meaningful results were obtained. following aspects were included in this dissertation : the structure of thin films is analyzed by rheed

    本論文基於激光分子束外延的基本原理，以高能電子反射為主要監測工具，對氧化物薄膜特別是鐵電氧化物薄膜異質外延過程中應變行為及其控制方法進行了系統的研究，並取得了一系列有意義的結果，主要包括以下內容:利用反射高能電子衍射（ rheed ）的信息對薄膜結構進行分析。
  9. Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway

    摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。
  10. Of particular interest are " memory cytotoxic t cells ", because people infected with certain viruses, especially cytomegalovirus ( which most of us have, in fact ), accumulate a huge number of this type of immune cell that is specifically reactive against proteins encoded by that virus

    特別有趣的是"記憶細胞毒t細胞" ，因為被某些病毒感染的人，特別是被細胞巨化病毒感染的人（實際上我們大部分人都被它感染） ，大量積累特異地起反應來抗擊該病毒編碼的蛋白質的此類免疫細胞。
  11. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  12. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  13. Studies on piezoelectric immunosensors based on plasma - polymerized film. ( abstract ) the piezoelectric immunosensors posses high sensitivity of response to mass and high specificity of bio - chemical reaction as well

    壓電免疫傳感器具有壓電質量響應的高靈敏性和生化反應的高特異性，在生物傳感器的研究中日益受到重視。
  14. Piezoelectric sensing technique has been applied to many fields, including analytical chemistry, life science, environmental monitoring and surface sciences because of it ' s small size, high sensitivity, simplicity, low power consumption and broad sensing spectrum. piezoelectric immunosensors possess high selectivity provided by antibodies or antigens and sensitivity response directly to the mass change of proteins. immobilization technologies of biomolecules are of paramount importance in order to preserve their biological activity

    壓電免疫傳感技術利用壓電晶體與抗原抗體特異性免疫反應結合的特徵，通常在壓電石英晶體表面先固定抗原（抗體） ，響應溶液中抗體（抗原）引起的質量、密度和粘度等性質的變化，它兼具有壓電質量響應的高靈敏性和生物化學反應的高特異性。
  15. Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years. however, a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly. therefore, it is a challenging task to develop strategies for improving the detection limit sensitivity of spr. in this paper, authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex. it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity. this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex

    將鏈霉親和素-生物素系統用於表面等離子體共振免疫傳感的信號放大，實時檢測了人免疫球蛋白g higg的蛋白濃度。發生免疫反應的傳感片和生物素化抗體反應后，傳感片表面的一層生物素分子隨后與鏈霉親和素-生物素化抗體復合物中的鏈霉親和素的活性位點發生親和反應，從而使傳感片表面特異健合的物質質量顯著增加，大大提高了免疫檢測的靈敏度和檢測限。免疫反應經放大后，可檢測0 . 00510g ml濃度區間內的higg 。
  16. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  17. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  18. Tissue sections from every animal were double - labeled with the antibodies of map - 2, cox - 2, gdnf, caspase - 3 and either the neuron - specific antibody neuronal nuclear protein ( neun ) or the astroglial - specific marker glial fibrillary acidic protein ( gfap ). we carried out a series of research to explore the effects and mechanism of map - 2, cox - 2, gdnf, caspase - 3 during tbi and trie d to provide some useful theory basis for both the treatment of tbi in the practice and forensic medicine

    並通過上述指標分別與神經元特異性標志物神經元核蛋白（ neuronalnuclearprotein ， neun ）和星形膠質細胞特異標志物膠質纖維酸性蛋白（ glialfibrillaryacidicprotein ， gfap ）進行免疫組織化學雙染色，探討腦損傷后神經元及神經膠質細胞反應性變化情況及其分子生物學機制，以期為腦損傷研究提供有益的數據材料，也為以上指標在法醫學實際檢案的應用提供必需理論依據。
  19. Acute phase respo - nse mediators can act on the hepatocytes by non - specific stimulation, which may lead to the changes of the gene expression and protein synthesis

    在一些非特異性因素的刺激下，急性時相反應介質與肝細胞反應引起肝細胞大量分泌急性時相反應蛋白，參與機體免疫。
  20. For the first time, the energy band configuration of p - si / n - bn heterojunctions were studied and drawn up. the study of the current - voltage ( i - v ) characteristics showed significant rectifying behavior with reverse saturation current of 3. 7 x 10 ~ 7a, breakdown voltage of 30v and turn on voltage of 14v. the capacity - voltage ( c - v ) characteristics are studied too

    研究了p - si / n - bn異質結的-特性，測試表明異質結具有明顯的整流特性，反向飽和電流3 . 7x10 「 』 a ，擊穿電壓高達30v導通電壓為14v ，應用p七en異質結能帶圖對其卜v特性做出了定性解釋。
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