甘藍菌 的英文怎麼說
中文拼音 [gānlajūn]
甘藍菌
英文
cauliflower fungus-
The hypocotyl segments of cabbage were used as the explants and cultured on a ms medium supplemented with plant hormone, which was established a cabbage regenerated system. the differentiation rate of adventitious buds was above 80 %. in this study, ubic gene was transformed to cabbage through agrobacterium mediated system
同時,採用甘藍無菌苗的下胚軸為外植體,以ms為基本培養基,分別用直接分化再生系統和愈傷再生系統誘導不定芽的分化,建立了高效穩定的再生系統,不定芽分化頻率達80以上。Effect of processing time on the transformation efficiency of brassica oleracea mediated by agrobacterium tume faciens
處理時間對農桿菌介導甘藍轉化中抗性芽分化率的影響The constuction of plant expression vector, tissue culture of cabbage and gene transformation conditions were studied to obtain ubic transformants. ubic gene was obtained from the escherichia coli. by pcr amplication and was ligated to puc118 vector
為獲得轉ubic基因甘藍,對植物基因工程載體的構建、甘藍的組織培養以及基因轉化條件進行了研究。通過pcr方法從大腸桿菌基因組中擴增得到了ubic基因,擴增產物克隆到puc118載體,轉化大腸桿菌jm109 。
分享友人