甜菜苷 的英文怎麼說
中文拼音 [tiáncài]
甜菜苷
英文
betanin(e)-
Water soluable, it is a type of plant derived pigment from beetroots
水溶性天然植物染料,是由甜菜根中提取的甜菜苷和異甜菜苷構成的物質。Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained
再次,根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物,與atp結合蛋白的上游簡並引物組合,經pcr擴增獲得2 . 1kb的條帶,測序后通過blast比較,結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。Beth showed 56 % identity to the opud of bacillus subtilis and belonged to bcct family. its putative promoter region was highly homologous to b - dependent promoter of b. subtilis. a 2. 6 kb fragment including the beth gene was subcloned into puc18 and transformed into the e. coli mkh13, colonies appeared on the plate of the selective m9 medium
將包括整個beth基因orf框及可能的啟動子核苷酸序列在內的2 . 6kb的片段克隆到puc18載體上,轉入到大腸桿菌甘氨酸甜菜堿缺失株mkh13中,使該菌株能夠在含甘氨酸甜菜堿的高鹽m9培養基上生長,而對照實驗不能生長。
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