生瘤桿菌 的英文怎麼說
中文拼音 [shēngliúgǎnjūn]
生瘤桿菌
英文
bacillus tumefaciens-
In addition, it was also found that this protein shares limited but significant homology with the sam - dependent methyltransferase of mesorhizobium sp. bnc1 ( 32 % similarity ), and the similarity of its 303 - 362 region to the 160 - 220 domains of l11 methyltransferases of e. coli ( prma ) is 41 %. it is suggested that methylation of l11 resulted in effects of noea on nodulation of 042bm
發現noea與中慢生根瘤菌（ mesorhizobiumsp . ） bnc1可能的sam -依賴性的甲基轉移酶相似性為32 ，而其303 - 362區域與大腸桿菌（ escherichiacoli ）的核糖體50s亞基的l11蛋白甲基轉移酶（ prma ）的160 - 220結構域的相似性達到41 。Helicobacter pylori planting in stomach mucosa is now recognized as the most widespread human pathogen. approximately half of the world ' s population is infected. the infection of h. pylori is highly associated with chronic active gastritis, peptic ulcers, gastric adenocarcinoma and lymphoma of the mucosa - associated lymphoid tissue ( malt ). in 1994, who ranked h. pylori as i grade carcinogen
幽門螺桿菌（ helicobacterpylori ， hp ）是定植於人胃粘膜的重要致病菌，全球感染率高達50以上，與慢性胃炎、胃十二指腸潰瘍及胃粘膜相關淋巴組織淋巴瘤的發生、發展密切相關， 1994年世界衛生組織（ who ）將幽門螺桿菌定為類致癌因子。Oligochitosan is not only water - soluble, non - toxic, biocompatible but also possesses versatile functional properties of chitin and its derivatives, such as polyelectrolite properties, the presence of reactive functional groups, gel - forming ability, high adsorption capacity, bacteriostatic, fungistatic and antitumour influence, and thus it has attracted more and more attention in the field of biology. previous investigations have shown that chitin and its derivatives have immunoenhancement activities
殼寡糖是由3 10個n -乙酰氨基葡萄糖殘基或氨基葡萄糖殘基通過- 1 ， 4 -糖苷鍵連接而成的寡糖，它具有良好的水溶性、組織兼容性，在生物體內易降解，同時也具有抑菌、抗腫瘤、調血脂、調節免疫及活化腸道雙歧桿菌等多種生理功能，因此已成為眾多領域的研究熱點。2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin
二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。The promoter - probe vector phn117 in e. coli and phn127 in g " were further constructed by removing promoter while keeping sd sequence from phn115. a teta / tetr bidirectional promoter fragment from pbr322 was respectively cloned into phn117 and phn127 and the resulted colonies were all fluorescent
並經插入pbr322上665bpteta tetr雙向啟動子片段后得到的轉化子均發綠色熒光驗證，實現了wtgfp在大腸桿菌和華癸中生根瘤菌中的組成型表達。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。And the fusion protein could been purified quickly by thiobond ? resin. this was key step to produce cmiv largely by fermentation. meanwhile, the structure of the mutation ii of cmiv showed a new way to design peptide of cecropin which have the targeting sequence to tumor cell
以上研究的意義： 1 、初步解決了抗菌肽cmiv突變體在大腸桿菌中高效可溶性表達的問題，為進一步大量生產cmiv基因突變體打下基礎； 2 、為進一步設計具有特異導向性的抗菌抗腫瘤cmiv突變體基因和表達以及以後進行動植物轉化打下基礎。In the aspect of microfilms, we isolated four bacteria which can induce the settlement of s. canopus larvae. they are h - 9 ( vibro ), h - 6 ( vibro ), h - 15 ( achromobacter ) h - 13 ( achromobacter ). h - 4 ( vibro ) can inhibit the settlement of larvae
在微生物粘膜方面，本文共篩選出4株單菌菌株對冠瘤海鞘幼體的附著有促進作用，它們分別是：弧菌屬的h - 9和h - 6 ，無色桿菌屬的h - 15和h - 13 。Combining the analysis of the conservative region of 16s rrna gene of prokaryote, such as e. coli, rhizobia and several frankia strains, we designed several sets of primers to amplify the 16s rrna gene of the frankia strains tested. through tentative experiments with these primers, we screened out primers uf / ur and ec27f / frl717r
通過比較已發表的原核生物，如大腸桿菌、根瘤菌和弗蘭克氏菌的16srrna基因全序列的保守區，設計了8對引物並篩選出可適用於擴增13株供試菌株16srrna基因接近全長序列的引物： uf ur和ec27f fr1717r （產物大小約1500bp ） 。分享友人