病毒中和試驗 的英文怎麼說

中文拼音 [bìngdúzhōnghéshìyàn]
病毒中和試驗 英文
viral neutralization test
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 試 : 名詞（古代占卜用的器具） astrolabe
  • 驗 : 動詞1. （察看; 查考） examine; check; test 2. （產生預期的效果） prove effective; produce the expected result
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  • 試驗 : trial; experiment; test
  1. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  2. If ip animal health analysis methods - detection of antibodies against mucosal disease by virus neutralisation test and immunochemistry on cell cultures if or ip

    動物健康分析方法.用病毒中性試驗和在細胞上培養
  3. Animal health analysis methods - detection of antibodies against classical swine fever by virus neutralisation test and immunochemistry on cell cultures if or ip

    動物健康分析方法.病毒中性試驗和細胞培養免疫化學檢測典型豬瘟的抗體
  4. Micro - serum neutralization test for swine vesicular disease virus

    豬水庖病病毒微量血清中和試驗
  5. Micro - serum neutralization test, virus isolation and identification for bluetongue

    藍舌病微量血清中和試驗及病毒分離和鑒定方法
  6. The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses

    同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。
  7. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  8. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  9. Standard test method for neutralization of virucidal agents in virucidal efficacy evaluations

    殺病毒劑功效評價中殺病毒劑中和作用的標準試驗方法
  10. The neutralization test is based on the neutralization of virus by specific antibodies found in the serum.

    中和試驗是基於病毒可被存在於血清中的特異性抗體。
  11. Animal health analysis method - detection of antibodies against viral haemorrhagic septicemia of salmonidae by virus neutralisation test

    動物健康分析方法.用病毒中和試驗測定鮭科病毒溢血敗血病抗體
  12. Animal health analysis method - detection of antibodies against infectious haematopoietic necrosis of salmonidae by virus neutralisation test

    動物健康分析方法.用病毒中和試驗對感染鮭科造血壞死抗體的測定
  13. 8 schmaljohn c s, and dalrymple j m. hantaviruses, encyclopeolia of virology, vol. 2, ( edited by r. g. webster and a. granoff ). p544

    7姚小劍，俞永新，安祺.檢測流行性出血熱病毒滴度和中和抗體的半微量空斑減少中和試驗.病毒學報， 1988 ， 4 （ 4 ） 347 - 350
  14. Serum neutralization test for duck virus hepatitis type virus

    鴨病毒性肝炎型病毒血清中和試驗
  15. Animal health analysis method - detection of antibodies against border disease by virus neutralisation test

    動物健康分析方法.用病毒中和試驗檢測抵抗近似病癥的抗體
  16. Animal health analysis methods - detection of antibodies against aujeszky ' s disease by the virus neutralisation test

    動物健康分析方法.用病毒中和試驗檢測假狂犬病抗體
  17. Animal health analysis method - detection of antibodies against border disease by virus neutralisation test and immunofluorescent revelation

    動物健康分析方法.用病毒中和試驗和免疫熒光顯示法檢測抵抗近似病癥的抗體
  18. Marc 145 cells were suitable for primary isolation and propagation of avian rotavirus. trypsin appeared essential for the enhancement of infectivity and the occurance of cytopathic effect ( cpe ). serum neutralization ( sn ) n electron microscopy ( em ) and analysis of genomic rna were done to identify and confirm the identity of rotavirus

    根據病毒形態、基因組的聚丙烯酰胺凝膠電泳、病毒中和試驗、動物回歸試驗，對分離的病毒進行了鑒定，並對分離病毒的生物學特性和理化特性作了部分研究。
  19. Detection antibodies against foot and mouth disease virus - protocol of micro - serum neutralization test

    口蹄疫病毒抗體檢測方法.微量血清中和試驗
  20. The results revealed that antigenic drift had occurred in virus strains of the same h9n2 subtype in the north of henan province during 1998 - 2002. h9n2 subtype aivs were stable from 1998 to 1999, since the mutual protection between 98a5 and 99s approached to 100 %, which was consistent with results in hi and vn tests

    1998年和1999年h _ 9n _ 2亞型禽流感病毒較穩定， 98a5和99s毒株間的保護力接近100 ，在hi 、雞胚中和、細胞中和試驗結果的相關性均在0 . 74以上，表明兩毒株間的抗原性相近。
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