病毒提純 的英文怎麼說

中文拼音 [bìngdúdīchún]
病毒提純 英文
purification of virus
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 提 : 提動詞（垂手拿著） carry （in one's hand with the arm down）
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  3. Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified

    為建立對豬傳染性胃腸炎快速有效的診斷方法，並試圖在預防上提供有效的免疫制劑，本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化；並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。
  4. The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev

    本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。
  5. The gold particles and virus could be seen binded together in the electronic microscope, which indicated the activity of purified igg was high. clinical application of hyperimmunalserun was used to treat dogs with clinicalsigns compatible with canine distemper and parvovitus enteritis

    將純化的igg與提純的cdv 、 cav病毒反應後分別與膠體金標記的spa結合，在電鏡下可清楚地觀察到病毒與膠體金顆粒的結合，說明提取的igg的效價、活性較高。
  6. Our efforts were taken to lay the foundation of further studies on cloning and function of new genes in fish. the construction and evalution of the a, gtlo cdna library of grass carp leukocytes are described. total rna was extracted from head - kidney leukocytes using single - step method of rna isolation by acid guanidinium thiocyanate - phenol - chloroform extraction

    本文第一部分取病毒感染36小時后的草魚頭腎並分離白細胞，用改進的異硫氰酸胍一步法從其中提取總rna ，磁珠法分離純化mrna ，電泳檢測其質量。
  7. Biopsies of these lesions reveals intranuclear inclusions in squamous epithelial cells indicative of herpes simplex virus esophagitis

    病變活檢顯示，鱗狀上皮細胞核內包涵體提示為單純皰疹病毒性食管炎。
  8. Such " punched out " ulcers are suggestive of herpes simplex infection

    此種穿透性性潰瘍提示有單純皰疹病毒的感染。
  9. Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified

    為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。
  10. A dot - enzyme - linked immnosorbent assay ( dot - elisa ) for the detection of antidodies against newcastle diesease virus ( ndv ) was established by using purified ndv and self - made enzyme - labeled anti - chicken igg, the mehod was also evaluated through it ' s application

    本研究用提純的新城疫病毒和自製的酶標抗體建立了檢測雞新城疫抗體的dot - elisa方法。試驗中用f _ （ 48 ） e _ 9株和lasota株提純抗原並對兩者進行了比較。
  11. Different hosts " response suggested that tumv - sd1 could infect plants of 10 species in 3 families. tumv - sd1 formed pine - wheel inclusion bodies in plant cells. the coat protein of the tumv - sd1 contains 3 components whose estimated molecular weight are 45kd, 38kd and 14kd respectively

    寄主反應特性表明， tumv - sd1 6能侵染3科10種植物， tumv - sd1在寄主細胞內形成風輪狀內含體，外殼蛋白為3組分，分子量分別為45kd 、 38kd和14kd ；提純的病毒粒體為長線條狀。
  12. Differential centrifugation purification and electron microscope examination of avian encephalomyclitis virus

    應用差速離心法提純禽腦脊髓炎病毒及電鏡觀察
  13. Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis. the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation, 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain. referred to the published sequence two primers were designed and synthesized

    給4周齡spf雛雞人工接種雞傳染性法氏囊病病毒hb - bp毒株， 48小時后採取法氏囊，用雙licl分級沉澱法提純全長基因組dsrna ，設計一對引物，通過rt - pcr方法進行了體外擴增，獲得hb株a節段基因全長cdna 。
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