病毒酶 的英文怎麼說

中文拼音 [bìngdú]
病毒酶 英文
viral enzymes
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Some viruses contain lipids, carbohydrates and special enzymes.

    某些病毒還含有脂類、碳水化合物和酶類。
  3. Change of catalase activity in interaction of tobacco and cucumber mosaic virus

    煙草與黃瓜花葉病毒互作中過氧化氫酶活性的變化
  4. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. Both myxoviruses and lysosomes are equipped with enzymes which can degrade glycoproteins.

    粘液病毒和溶解體均裝配某些能分解糖蛋白的酶。
  7. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  8. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  9. The homology of the other two motifs ( v and vi ), which are also quite conserved in other helicases, were lower than 30 %. the hasn pv helicase protein had considerable amino acid sequence similarity with other baulovirus helicases ( 58 % ), and the highest ( 66 % ) with semnpv and the lowest with xcgv. ( 43 % ). hasnpv helicase was the first helicase reported in single - nucleocapsid nucleopolyhedrovirus there are 5 homologue regions ( hrs ) in hasnpv genome which may play important roles in the viral replication

    同源性比較發現hasnpv解螺旋酶的氨基酸序列與甜菜夜蛾核多角體病毒（ spodopteraexiguemnpv ， semnpv ）的解螺旋酶具有最高的同源性（ 66 ） ，與xestiac - nigrum顆粒體病毒（ xcgv ）解螺旋酶同源性最低（ 43 ） ， hasnpv解螺旋酶基因是第一個報道的單粒包埋核多角體病毒的解螺旋酶基因。
  10. The reverse transcriptase isolated from human leukemic cells resembles that from rna tumour viruses in several respects.

    從人白血病細胞分離得到的遞向轉錄酶在如下幾方面類似於RNA腫瘤病毒逆向轉錄酶。
  11. Enzyme - linked immunosorbent assay for avian leukosis virus p27 antigen

    禽白血病病毒p27抗原.酶聯免疫吸附試驗方法
  12. Construction of adenoviral vector for luciferase driven by htert core promoter modified with myc - responsive elements

    核心啟動子引導熒光素酶表達的腺病毒載體
  13. Construction and expression of rnase - resisting virus - like particles containing partial sequence of alpha - fetoprotein messenger rna

    部分序列的耐核糖核酸酶病毒樣顆粒的構建和表達
  14. Inhibitory effect of ornithine decarboxylase and s - adenosylmethionine decarboxylase biantisense adenovirus on colorectal cancer cells

    腺苷甲硫氨酸脫羧酶雙反義腺病毒對大腸癌細胞生長的抑制作用
  15. The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )

    以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。
  16. Correlation between signal cutoff ratios of anti - hcv enzyme immunoassay and their true positivity in blood donors

    獻血員丙型肝炎病毒抗體酶免疫法檢測試劑的測量值與其真陽性的相關性
  17. A protease is key to the replication of the virus

    蛋白酶是阻止非典病毒變異的關鍵物質。
  18. One such drug has shown promise in clinical trials at controlling cold symptoms by inhibiting the protease in rhinoviruses like that in the sars virus

    其中一種藥物在臨床試驗中已顯示出，它可以抑制鼻病毒中蛋白酶而控制感冒的癥狀， sars病毒也有相似蛋白酶。
  19. As indicated above, several m1gs that cleaved hcmv ul54 mrna segments in vitro were successfully designed and constructed. our studies demonstrates the utility of this ribozyme m1gs for antiviral application

    我們的研究成功地利用引導序列，將核酶rnasep催化亞單位m1rna構建為序列識別的核酶m1gs ，證實了核酶在抗病毒方面的應用價值。
  20. The main bioactivities include antibacterial, antimicrobial, antitumor, antiviral activity, enzyme and enzyme inhibition, and antitumor activity is more important

    其生物活性包括：抗菌、抗腫瘤、抗微生物、抗病毒、酶及酶的抑制活性等，其中以抗腫瘤活性最為重要。
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