發根農桿菌 的英文怎麼說
中文拼音 [fāgēnnónggǎnjūn]
發根農桿菌
英文
agrobacterium rhizogenes-
Transformation of glycyrrhiza inflat bat by agrobacterium rhizogenes r
發根農桿菌轉化脹果甘草的研究In this paper, genetic transformation systems by agrobacterhim rhizogems was established and optimized and high quality regenerating system was selected. the products of indigotin and indirubin in regenerating plantlets had been detected by hplc. the main studies and results were reported as follows : 1 establishment and selection of hairy root clones taken cotyledons of autotetraploid indigoblue woad as explants and hairy roots induced successfully from them by agrobaterium rhizogenes strains a4 and r1000
本文利用發根農桿菌rit - dna建立並優化了四倍體菘藍的遺傳轉化體系，並對再生植株的主要代謝產物靛藍、靛玉紅進行了含量檢測，篩選了優良無性系，也為實現外源抗蟲、抗病等基因的導入奠定了基礎。Adventitious shoots could be rooted and formed regenerated plants on 1 / 2 ms medium supplemented with naa0. 4mg / l. hairy roots could be induced from the basal surface of the explants 8 days after inoculation of a. ficoidea cv. " ruliginosa " leaf explants with a. rhizogenes atcc 15834
紅龍草葉片外植體被發根農桿菌atcc15834感染8d后，從形態學下端葉脈處陸續分化出乳白色的不定根， 21d后，葉片外植體的生根率高達92 . 5 。In this study, the screening and identification of the different agrobacterium rhizogenes strains were carried out using physiological, chemical, molecular and bioassay methods, and the vigor of different strains was also compared based on their characters ; the transformation of torenia mediated by agrobacterium rhizogenes, the hairy root formation and its regeneration were importantly studied
本實驗研究了（ 1 ）發根農桿菌的生理生化、分子生物學鑒定及生物檢測和活力比較； （ 2 ）發根農桿菌介導的藍豬耳遺傳轉化和藍豬耳毛狀根的植株再生，為藍豬耳的遺傳轉化和其它分子生物學研究奠定基礎。The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern
通過實驗和對結果的數據分析，得出下面的結論：發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株，它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率（ 60 ） ，最適的菌株感染濃度od _ （ 600 ）為0 . 7 ，溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養，以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。Aimed at developing a set of methods efficient in inducing the hairy roots with agrobacterium rhizogenes from trichosanthes bracteata voigt to meet needs for hairy roots for bioreactor culture research and application ; and also in order to investigate the roles of explants, agrobacterium rhizogenes, affecting factors and their magnitude and the underlying mechanism in the transformation, therefore to determine the optimum explants, pre - culture time, tested strain concentration, infection time, co - culture time, affecting factors and their effects working together and to obtain the optimum transforming condition by performing orthogonal test, this project was launched. with cotyledons and stems from trichosanthes bracteata voigt plantlets as explants, agrobacterium rhizogenes r1601 and r1000 as infection agents, transformation was performed using different methods and under various circumstances
為了建立一種有效的誘導大苞栝樓發根的方法及其發根培養體系，以適合於發根生物反應器擴大培養研究和應用基礎的需要；本研究對誘導發根的外植體在發根農桿菌和環境因子等影響作用下進行發根的誘發及其可能的機理進行探討，從而找出用於發根誘導和培養的最佳外植體、預培養時間、發根農桿菌濃度、感染時間和共培養時間、誘導條件，並通過正交試驗得出優化的誘導條件。Agrobacterium a genus of soil bacteria, the species a. tumefaciens being the causative agent of crown gall, a type of tumor in plants
根癌農桿菌：土壤中存在的一種農桿菌，可以感染雙子葉植物的受傷組織誘發一種植物的腫瘤冠癭瘤。It shares less than 25 % homology with the epsp synthases in class ii. it also shares 24. 5 % homology with the epsp synthase isolated by monsanto company and does not contain any conservative sequences and mutational sites protected by patents. all of the above shows great potential for future development
與class的epsp合成酶同源性低於25 ，其中與monsanto公司分離的根癌農桿菌（ agrobacteriumtumefaciens ） cp4來源的epsp合成酶同源性僅為24 . 5 ，且不含有專利保護的保守序列和突變位點，顯示了良好的開發應用潛力。In this paper, we transferred ced - 9 gene, an anti - apoptosis gene, into tobacco and rice by agrobacterium tumefaciens eha105. the results are showed as follows : 1
Ced - 9是發育pcd的負調控基因，本課題將ced - 9基因通過根癌農桿菌（ agrobacteriumtumefaciens ） eha105介導轉化煙草和水稻，獲得以下結果： 1The purpose of this study is to provide theoretical and technical support for torenia transgenic hairy root and other molecular researches used by torenia. the comparation and identification of agrobacterium rhizogenes strains were investigated using 3 - ketolactose test, the differential acid production assay and the motility assay
採用3 -酮乳糖產物法、差異酸生成實驗和游動性實驗鑒定6種發根農桿菌供試菌株a4 、 r1205 、 r1000 、 r1601 、 r1022和15834 。Sonication - assisted agrobacterium - mediated transformation of cucumber
超聲波輔助發根農桿菌對黃瓜遺傳轉化的影響This paper reports hairy roots induced by agrobacterium rhizogenes and production of regenerative plants from hairy root cultures in maize
本文利用發根農桿菌遺傳轉化玉米，通過毛狀根的誘導、毛狀根的分化培養獲得再生植株。But the concentration of a4 at 40 h was higher than that of other strains. four strains ( a4, r1205, r1000, r1601 ) in this study were agrobacterium rhizogenes according to pcr assay
利用pcr方法鑒定結果表明，所有供試菌株中， a4 、 r1205 、 r1000和r1601為含ri質粒的發根農桿菌，而r1022和15834未出現陽性結果。Tissue culture and plantlet regeneration from leaf explants of a. ficoidea cv. " ruliginosa " and its genetic transformation with agrobacterium rhizogenes atcc 15834 were investigated as well as changes in the contents of sucrose, nitrogen and phosphate in liquid medium during the hairy root cultures
本文研究了紅龍草（ altemantheraficoideacv " ruliginosa " ）葉片的組織培養、發根農桿菌（ agrobacteriumrhizogenes ） atcc15834對紅龍草的遺傳轉化以及毛狀根液體培養過程中培養液某些營養成分的消耗變化。Presently, the scientist have made out some successes by using biotechnology and molecuology, for example, planting out regeneration shoots, culture suspended cell in ferment tank, screening mutant tissue with high content of flavonoids, cloning and sup - expression of key enzyme, restraining derivative pathway by anti - dna or anti - rna and inducing genetic transformated hairy root
通過培養水母雪蓮的發狀根將是獲得雪蓮類黃酮極有前途的方法，目前還是一項待填補的空白。發根農桿菌（ agrobacteriumrhizogenes ）是一種革蘭氏陰性土壤細菌，細胞內有200kb左右的雙鏈閉環dna ? ri質粒（ rootinducingplasmid ） 。More interesting, the content of secondary metabolite always increased markedly in hairy root. so the transgenic system by ri plasmid would be wildly used in plant breeding, plant gene engineering and pharmaceutical element production
發根農桿菌遺傳轉化體系的這一特點在作物育種、植物基因工程、次生代謝物工廠化生產上有廣闊的應用前景。分享友人