白泳毛 的英文怎麼說
中文拼音 [báiyǒngmáo]
白泳毛
英文
cock schlappen white-
The blood of each rabbit was collected at days 0, 7, 14, 2l, 30. the antibody titers were evaluated by k - agglutination test. the results showed that lower agglutination titers was observed at day 7 and up to higher levels over 8000at day 21 induced by the vaccine of recombinant fused protein
將融合基因工程菌株在營養肉湯中培養，離心後上清用0 . 1mmgcl _ 2沉澱，離心，提取表達產物。經sds - page電泳證明表達產物為纖毛蛋白與il - 2的融合蛋白。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0
菜心go同工酶的sds - page和sds -毛細管電泳（ ce - sds ）顯示，該酶除了含40kd主帶外，還有很淺的66kd帶，和之前我們提出的菠菜go同工酶含40kd酸性亞基和66kd堿性亞基相似； sos - page和ce - sds電泳中，無論加入-巰基乙醇與否， go同工酶都只有40kd主帶和66kd淺帶，表明菜心go同工酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連；用制備性sds - page法獲得菜心go同工酶的40kd亞基，並和菜心go同工酶一起測定其氨基酸組成，該go同工酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ，表明40kd亞基可能是個酸性蛋白，而66kd帶則是個堿性蛋白。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態；應用原子力顯微鏡掃描，比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別；用透射電鏡觀察細胞壁完整性，以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變，並通過實驗分析多種物質和因素對潛生體形成的影響。Determination of the unbound clozapine concentration in human serum albumin ( has ), human plasma, rabbit serum and plasma samples by capillary electrophoresis in the frontal analysis mode ( ce / fa ) was developed
摘要採用毛細管電泳-迎頭分析模式體外實驗測定了人血清白蛋白溶液、人血漿、兔血清和血漿樣品溶汲中游離氯氮平的濃度。A review of the applications and prospects of capillary electrophoresis with electrochemical detection to the analysis of biomolecules such as amino acids, protein, dna and hydrocarbon covering, the years from 1994 to 2004, was presented in this paper with 55 references cited
摘要對近年來毛細管電泳電化學檢測在生物分子（氨基酸、蛋白質、脫氧核糖核酸和糖等）分析中的應用進展作出綜述，展望了電化學檢測在毛細管電泳中的應用前景（引用文獻55篇） 。3. the hair keratin polymorphisms differ with the sampling sit on the body. for different individuals of the same species, an identical keratin fingerprint will be obtained if the hairs come from the same part of body, and vis versa
毛角蛋白多態性與取樣部位有關，同種不同個體間，如果被毛來自身體的相同部位，角蛋白電泳結果一致，若被毛采自身體的不同部位，蛋白譜帶存在差異。分享友人