白點感受性 的英文怎麼說
中文拼音 [báidiǎngǎnshòuxìng]
白點感受性
英文
flake susceptibility- 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
- 點 : Ⅰ名詞1 （液體的小滴） drop （of liquid） 2 （細小的痕跡） spot; dot; speck 3 （漢字的筆畫「、」）...
- 感 : Ⅰ動詞1 （覺得） feel; sense 2 （懷有謝意） be grateful; be obliged; appreciate 3 （感動） move; t...
- 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
- 白點 : fish eye/ flake
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After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。Subsequently, the binding of ( 35 ) ~ s - cam and a. thaliana protoplasts were processed, including association, dissociation, competitive and saturation analysis. the results show the binding of ( 35 ) ~ s - cam and protoplasts is rapid, reversible, saturable and sensitive to proteinase ; otherwise the specificity of binding was assessed with different competitors. k _ ( d ) of 9. 2 nm and about 25, 000 binding sites per protoplast were gained through saturation analysis
隨后，對~ （ 35 ） s - cam和擬南芥原生質體的結合進行了受體學分析，包括結合、解離、競爭性和飽和分析，結果顯示~ （ 35 ） s - cam和擬南芥原生質體的結合具有結合快速性、可逆性、競爭特異性和濃度飽和性，從飽和分析可以得出k _ d約為9 . 2nm ，每個原生質體表面大約含有25 ， 000個結合位點，並且其結合對蛋白酶敏感。
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