相序列分析 的英文怎麼說

中文拼音 [xiāngxùlièfēnxī]
相序列分析 英文
facies sequence analysis
  • 相 : 相Ⅰ名詞1 （相貌; 外貌） looks; appearance 2 （坐、立等的姿態） bearing; posture 3 [物理學] （相位...
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  1. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  2. A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101. the complete sequence analysis of the plasmid show that it consists of 3176 bps, which contains four putative orfs. orf1 encodes a putative protein and is highly similar to repa which functions in replication

    從屎腸球菌df101菌株中分離到隱秘的小質粒pefr ，全序列分析顯示質粒pefr由3176bp組成，編碼四個推定的orf ， orf1編碼的一個推定的蛋白和復制有關的repa有很高的相似性。
  3. The first part of this study is polyphasic taxonomy analysis of the thirteen strains which have herbicidal activities. the polyphasic taxonomy methods include morphology, cytochemistry, dna g + c mol %, phylogenetic analysis of 16s rdna sequences and physiological and biochemical experiments

    本實驗的第一部分對具有除草活性的13株放線菌（ 40001 40013 ）進行了系統的多相分類研究，採用了形態學、細胞化學組分、 dnag + cmol 、 16srdna序列分析及生理生化實驗等技術方法。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  6. Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant

    本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。
  7. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  8. According to the least twin multiplication to calculating the sensitivity index in several water production functions. thus, the writer obtains the fitted the value of the sensitivity index and the varied rule. at the same time, the writer puts forward a new method named rag a ( real coding based accelerating genetic algorithm ) and combines raga with dpsa to calculating the best irrigation system under the non - sufficient irrigation of well irrigation rice in sanjiang plain

    根據《隨機水文學》理論中的時間序列分析法，建立了適合三江平原井灌水稻需水量預報的非平穩時序隨機模型；通過分析降雨隨機特性，選定季節性時序隨機模型，建立了適合三江平原井灌水稻降雨預報的月平均降雨模型；根據最小二乘法，計算出幾種常用水分生產函數中的敏感指數及敏感系數，進而得到三江平原適宜採用的水分生產函數漠型及模型中敏感指數的變化規律；本文提出遺傳動態規劃法（ raga ? dp ） ，即採用改進的基於實數編碼的加速遺傳演算法（ realcodingbasedacceleratinggeneticalgorithm ，簡稱raga ）與動態規劃法（ dpsa ）相結合，推求非充分灌溉條件下三江平原井灌水稻的最優灌溉制度。
  9. Fachb440 was higher than that in the others. alignment based on deduced amino acid sequences indicated that spirulina fachb440 was different from that in other three samples of arthrospira. nucleotide sequence similarity among the three strains of the genus of arthrospira ( 96. 5 - 99. 6 % ) was higher than that between arthrospira and spirulina ( 78. 1 - 78. 5 % )

    Maximaouqdsm ）采螺旋藻（ spirulinafachb440 ） rubisco大亞基基因（ rbcl ）部分片段並進行了序列測定與分析，結果表明：螺旋藻（ spirulinafachb440 ）的gc含量比其他的節旋藻品系高；氨基酸序列分析發現螺旋藻與其他節旋藻品系的差異較大；螺旋藻與節旋藻核著酸序列相似性約為78
  10. In this study, actinomycetes isolated have been analyzed using polyphasic taxonomy technology including morphological and physiological tests, analysis of chemotaxonomy, dna g + c content and 16s rdna / rna sequencing

    採用形態學、細胞化學、生理生化、 dnag + cmol及16srdna序列分析等多相分類的技術對所分離的部分放線菌進行了系統的分類研究。
  11. Genetic diversity analyses of three wild populations of pinctada martensii dunker. using rapd technique

    月季切花衰老相關基因的差異顯示及其序列分析
  12. Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %

    本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。
  13. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  14. We choose the forest appearance tidiness, representative stands serving as standard plots, 40 stands were set up at qinling, hanzhong, huanghong in shaanxi respectively, measure every tree in the stands, measure the actual increase by the dominance tree, the time series model of individual age and diameter of quercus variabilis was established according to the actual diameter of quercus variabilis population by the fluctuant time series, the comparison of simulation and reality value of the every stand of quercus variabilis population diameter increase through the four models, the average simulation difference within 1. 5 %, the accuracy is 97. 8 % the simulation effect is better

    在陜西的秦嶺、漢中、黃龍地區選擇林相整齊、有代表性的地段作為標準地，設置樣方40個，對各樣方內的林木進行每木檢尺，通過優勢木解析的方法，測得栓皮櫟種群胸徑的實際生長量，運用起伏型時間序列分析，建立了栓皮櫟種群個體年齡與胸徑生長的時間序列模型，四個模型所得的各個樣地栓皮櫟胸徑生長的模擬值與實際值進行比較，其模擬平均誤差都在1 . 5 %以內，平均精度達到97 . 8 % ，模擬效果較好。
  15. A real physical system may involve many variables but only one or more of them can be detected by modem data collecting equipment in recent years, the technique of phase space reconstruction is frequently applied to analyse and process time series. its significance is that the topological characteristics such as fractal dimension can be obtained, on the basis of investigation and research about the technique of phase space reconstruction up - to - now, this paper is also devoted to develop a new method for the prombles of detecting deterministic chaos of time series obtained from experimental data

    一個實際的系統可能會涉及多個相變量，但在實際問題中只能得到部分相變量的信息，近年來人們發展了相空間重構方法，能夠通過單變量信息重構吸引子，這種方法在時間序列分析和處理中得到廣泛應用，其意義在於能在拓撲等價意義下恢復吸引子的拓撲特徵，本文第三章在國內外有關時間序列的相空間重構研究狀況基礎上，致力於發展對時間序列進行確定性檢驗的新方法，即研究時間序列的分佈規律和赫斯特指數，並在相空間重構的基礎上，提取吸引子的特徵指數。
  16. In this dissertation, sequences of rdna from eleven important phytoplanktonic strains collected at jiaozhou bay are amplified, cloned, sequenced and compared, and the phylogenetic relationships among them are analyzed using the ssu rdna sequences

    本論文首先對分離自膠州灣的十一種浮游植物的rdna序列進行了pcr擴增、克隆、序列測定、序列相似性分析，並利用測得的小亞基（ ssu ） rdna序列分析了這些浮游植物的系統進化關系。
  17. In the part i, the sequence of the cloned promoter osg6b " was first analyzed. osg6b " had a whole length of 1688 bp and 98 % homology to known sequence of promoter osg6b. its transcriptional start point, tata boxes and the consensus sequences " tgtgg " conserved usually in anther - specific promoters were identical to those of reported osg6b

    第一部分：對已克隆的啟動子osg6b 』進行的序列分析表明， osg6b 』全長1688bp ，與報道的osg6b有98的同源性，兩者在轉錄起始位點、 tatabox及其它花藥特異性啟動子共有的保守序列tgtgg完全相同。
  18. Based on the principles of network - centric warfare and the background of anti - ballistic missile operation, the present study describes the process and mission sequence of badms / nco anti - ballistic missile operation, analyses functional modules and functional nodes of badms / nco, designs the functional architecture of badms / nco with a three - layer logic network structure, analyses the components arid functions of three logic networks and their relations

    基於網路中心戰原理，以區域反彈道導彈為背景，描述了混編防空導彈網路化作戰系統反導作戰的作戰過程及其任務序列；分析了混編防空導彈網路化作戰系統的功能模塊和功能節點劃分；構建了以3層邏輯網結構為特徵的混編防空導彈網路化作戰系統功能體系結構，分析了3層邏輯網的組成與功能及其相互關系。
  19. 16s rdna sequence analysis of three strains of 050642, 060386 and 060524 whose id50 is above 1200 indicated they also belong to streptomyces genus and strain 050642 is suspected to be a novel streptomyces. spp with the highest similarity to streptomyces colicolor, at 95 %

    對三株有較強活性的菌株050642 、 060386和060524進行了16srdna序列分析，初步確定這三株菌都屬鏈黴菌屬，其中菌株050642與其親緣關系最近菌株的相似性僅為95 ，極有可能是鏈黴菌屬的一個新種。
  20. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
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