真核生物表達 的英文怎麼說

中文拼音 [zhēnhéshēngwùbiǎodá]
真核生物表達 英文
eukaryotic expression
  • 真 : Ⅰ形容詞（真實） true; genuine; real Ⅱ副詞1 （的確; 實在） really; truly; indeed 2 （清楚確實） cl...
  • 核 : 核構詞成分。
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 生物 : living things; living beings; organisms; bios （pl bioi bioses）; biont; thing; life生物材料 biol...
  • 表達 : deliver; express; show; voice; convey; communicate
  1. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  2. In order that bar gene efficiently transcript and translate in eukaryote, the sequence next to atg was modified by pcr

    為了目的基因在真核生物中有效的翻譯和表達，通過pcr方法對bar基因起始密碼子atg旁側序列進行改造。
  3. It has not only many advantages of the prokaryotic expression system such as growing quickly, manipulating easily, strong power to express and control foreign protein, but also the ability to make foreign protein modified in post - translation. so pichia pastoris gene expression system is more appropriate to express many eukoryotic proteins than prokaryotic system and has attracted more and more people " s attention on it

    它不僅具有原核表達系統生長快速，操作簡單，外源基因表達量大並受到嚴格調控等特點，還具有真核生物特有的蛋白質翻譯后修飾的功能，表達真核生物蛋白質具有原核表達系統無法比擬的優越性。
  4. The protein expressed by this system can be modified correctly after the translation. firstly, rna is extracted from fresh tissue of pig liver, which is amplified as the templet by two groups of primers, so the aim gene is obtained successfully

    針對ple這一真核生物的糖蛋白，本實驗選用invitrogen公司的phichiapastorismulti - copyexpressionsystem克隆表達ple ，使ple能夠得到正確的翻譯后加工和修飾。
  5. An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene, fnr gene ) in vero cells. interestingly, we found that the nontargeted mutation frequency induced by mnng was increased significantly, implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation

    利用反義核酸技術構建含反向插入9號片段的真核細胞表達重組體並轉染細胞，以獲得反義rna阻斷vero細胞中相應基因的表達，發現mn 』 ng誘發的非定標性突變頻率顯著增高，提示被阻斷的相關基因的表達產物可能參與抑制非定標性突變的發生。
  6. At the same time, vp4 gene was mutated in a certain point by pcr. the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly. a. tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l. guad according to the leaf disc procedure

    為便於基因操作，對外殼抗原蛋白vp4基因進行適當的修飾和改造：通過引物設計，利用pcr反應，在基因的起始編碼前引入有助於真核生物表達的kozak序列和限制性內切酶位點；使用套疊pcr對vp4基因進行定點突變，以便於將改造后的基因插入pbi121構建植物表達載體，通過直接轉化法，把pbi121 vp4轉入農桿菌eha105 ，構建了農桿菌工程菌。
  7. In the recent decade, more and more attention has been paid to the roles of acetylation in transcription regulation

    尤其是近十多年來，乙酰化在真核生物基因表達調控中的作用日益受到人們的關注。
  8. The study on the relationship between the acetylation and hsp gene transcription regulation will help us in understanding the mechanisms of eukaryotic transcription regulation

    因此，對乙酰化與熱休克蛋白基因表達調控關系的深入研究，將有助於我們對乙酰化參與的真核生物基因表達調控的理解。
  9. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  10. Applications of animal ' s growth hormone are mostly studied by means of protein type, up to now, no other reports about gh gene directly used in animals have been found except one paper about the transfection of human gh gene into mice. in this research, we studied the changes in the bullfrogs after they are separately injected with the recombinant bullfrog gh protein ( re - bfgh ), bullfrog gh plasmid ( vb / gh ), grass carp gh plasmid ( vgcgh ) and expression vector vr1020 while the 0. 85 % salt - water as the control, for the purpose to determine the possibility of that the eukaryotic expression plasmid vbfgh and vgcgh are expressed in adult bullfrogs and affect their growth rate and plasma gh. we hope the results will help developing a new approach to promote the animal growth

    本研究首次以兩棲動物牛蛙為研究對象，進行了其生長激素基因的克隆以及原核表達質粒與真核表達重組質粒的構建、重組牛蛙gh蛋白的生物活性和免疫活性檢測以及重組蛋白制劑和核酸制劑的制備及其體內促長作用等表達效應研究，研究目的在於求證重組真核表達質粒是否能在牛蛙中表達、其促長效應是否強于重組bfgh蛋白，為探索重組生長激素真核表達質粒能否替代重組gh蛋白作為動物促長基因制劑等研究奠定理論基礎。
  11. Rab proteins are a family of small molecular weight gtpase of ras superfmaily with characteristic molecular weight about 22 - 25 kd. rab gtpases are expressed in almost all eukaryotic cells, ranging from yeast to drosophila and to human. they are ubiquitously or tissue - specifically expressed and function in all of cellular aspects

    Rab蛋白是屬于ras蛋白超家族的小g蛋白（ smallg - proteinorsmallgtpase ） ，分子量大多在22 - 25kda之間，表達于從酵母、果蠅、小鼠到人類等各種真核生物中，發揮著各種各樣的功能；其在酵母中稱為ypt sec4p在高等真核生物中稱為rab 。
  12. A eukaryotic expression vector pcdna3. 1 - cptl was constructed by insert cp77 gene into the vector pcdna3. 1 which is used in nucleic acid immunization. the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal. immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body

    本研究通過限制酶將cpti基因片段從載體pbluel3上切下，插入真核表達載體pcdna3 . 1 ，構建了用於核酸免疫的真核表達載體pcdna3 . 1 - cpti ；質粒大量提取和純化后，通過肌肉注射的方法免疫balb c小鼠，基因表達產物刺激小鼠機體產生免疫反應，從而獲得了抗cpti蛋白的抗體。
  13. In the first of 1990s, a new kind of vaccine - dna vaccine gives a new way to solve the immunogenicity of cea. it is defined as plasmids encoding antigen protein which can elicit immune responses against the antigen through direct injecting into body and taken into by cells, then, it stimulates the body generate specific immune response through different routes

    核酸疫苗是指將含有編碼腫瘤相關抗原基因的真核表達質粒，直接接種到動物體內，然後被動物細胞攝取並表達出相應的抗原，通過不同途徑刺激機體產生針對該抗原的免疫應答，從而消除或抑制腫瘤細胞的生長。
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