短小桿菌 的英文怎麼說
中文拼音 [duǎnxiǎogǎnjūn]
短小桿菌
英文
bacillus pumilus-
Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達，而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate
將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。Was supplied. its essential characteristics are as follows : short rods, 2. 0x1. 6 ^ m in diameter, arranged singly, gram negative, facultatively anaerobic, having both a respiratory and a fermentative type of metabolism, oxidase negative and catalase positive
通過形態觀察及生理生化特徵測定發現菌株w12為革蘭氏陰性菌，短桿狀，對數生長期細胞大小為： 2 . 0 1 . 6 m ，單個排列；兼性厭氧，氧化酶陰性，發酵葡萄糖產酸產氣，不運動。Bacillus pumilus un - 31 - c - 42 was obtained by mutations of strain ba ( 06 ), which is an alkaline protease producer and separated from life waste in chengdu
短小芽孢桿菌（ bacilluspumilus ） un - 42 - c - 31是一株從成都市生活垃圾中分離，經過多次反復誘變后得到的堿性蛋白酶產生菌。The result of sequencing shows that this fragment contains an open reading frame of 1106 nucleotides and encodes 369 amino acids residues, which is part of a alkaline serine protease, with 98. 3 % homogeneity to a reported gene from bacillus pumilus
序列分析顯示，該片段是一個全長1106bp的orf ，可編碼369個氨基酸殘基的多肽，與短小芽孢桿菌堿性蛋白酶基因編碼區高度同源。A pair of primers, based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus, were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction
Sds 、 edta和pmsf對酶活力有不同程度的抑制作用。以4株芽孢桿菌的總dna為模版，利用擴增短小芽孢桿菌堿性蛋白酶基因的引物對它們進行pcr擴增。The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp. the fermentation liquor also showed good dehairing activity. the alkaline protease ( named dhap, dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography, ion exchange and gel filtration
通過cm - sepharosefastflow離子交換層析， deae - sepharosefastflow離子交換層析， sephacryls - 100 ， sephacryls - 200凝膠過濾層析，疏水層析等純化步驟對短小芽孢桿菌發酵液中的堿性蛋白酶進行了純化。High performance ion exchange chromatography was applied in studying qualitatively and quantitatively of bacteria, which was shown as follows : firstly, physio - biochemical characteristics of bacteria was investigated by ion exchange chromatography. for the first time spores and nutrient of bacillus pumilus had been separated successfully by chromatography. chromatographial behaviors of bacteria at different cultivating environment and different growth phase were also studied
本文利用高效液相離子交換色譜系統研究細菌學，探討了該方法在細菌定性、定量方面的應用，主要包括三個方面：首先，利用離子交換色譜系統表徵細菌生理、生態方面的變化，首次成功地在色譜上區分了短小芽孢桿菌的芽孢及營養體；考察了不同的培養環境對細菌色譜行為的影響及不同生長階段的細菌的色譜行為。The homologous analysis showed that the n - terminal sequence of dhap is identical to that of serine protease from another b. pumilus strain tyo - 67 and has high homology with those from other bacillus species. based on the homologous analysis, three pairs of primers were designed and synthesized
該序列與來自其它芽抱桿菌屬菌株的絲氨酸蛋白酶的n一末端氨基酸序列有較高的同源性，並和另一株短小芽抱桿菌的絲氨酸蛋白酶n一末端氨基酸序列完全一致。分享友人