碰蹄 的英文怎麼說
中文拼音 [pèngtí]
碰蹄
英文
interfere-
With the rhythmic tramp of hoofs, the smartly dressed cavalry in blue, and red, and green laced uniforms rode jingling by on black and chestnut and grey horses, the bandsmen in front covered with embroidery
時而可以聽見節奏均勻的馬蹄聲和馬刺的碰擊聲，這些穿著藍色紅色綠色的繡花制服的騎兵騎在烏黑色棕紅色青灰色的戰馬上，一些穿著繡花衣服的軍樂樂師站在隊列的前面。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。But what are you about ? the squadron cant advance ! roared vaska denisov, viciously showing his white teeth, and spurring his handsome, raven thoroughbred bedouin, which, twitching its ears at the bayonets against which it pricked itself, snorting and shooting froth from its bit, tramped with metallic clang on the boards of the bridge, and seemed ready to leap over the railings, if its rider would let it
「騎兵連沒法子走過去， 」瓦西卡傑尼索夫惡狠狠地露出潔白的牙，用馬刺刺著那匹好看的烏騅貝杜英，高聲喊道，那匹烏騅碰到刺刀尖，抖動著耳朵，打著響鼻，從馬嚼子上噴出白沫，鈴鐺丁零丁零地響著，馬蹄子踩著橋板，發出咚咚的聲音，假如騎馬的人允許，它似乎準備跨過橋欄桿跳下去。About three o'clock george's ear caught the hasty and decided click of a horse's hoof coming behind them at some distance, and jogged phineas by the elbow.
到三點鐘左右，喬治忽然聽見后遠遠地傳來了一陣急促而堅定的馬蹄聲，便輕輕碰了一下斐尼亞斯的胳膊。Out in force were the stars of the film. zhao tao, jia s veritable muse, cheng taisheng, the dashing male lead and wang hongwei, the xiao wu transplanted to beijing s city life. producer chow keung and the director himself also were on - hand for the press, as were stars such as rebecca pan and french actress sylvie testud
4月6日電影節閉幕這天，碰巧也是《世界》作中國全國巡迴首映的第二日，賈樟柯率領趙濤、成泰?及王宏偉等一眾演員，以及監制周強等人在香港見過電影節的觀眾后，便馬不停蹄繼續奔走廣州、北京和上海做宣傳。In this paper, we choose a reasonable basic area and a coordinate transform and give conditions that ensure the invariant set or hyperbolic invariant set exist in the bouncing ball model by using the mosers and zhou jianying ' s conditions respectively. thus, the existence of horseshoe of the bouncing ball model is solved effectively
本文對碰撞恢復系數為0 1的彈跳球模型，通過選取合理的基本區域和引入適當的坐標變換，分別利用moser條件和周建瑩提出的一組微分條件，給出了彈跳球模型存在雙曲不變集和不變集的條件，從而很好地解決了該模型馬蹄的存在性問題。In addition, guckenheimer and holmes spent a great of length to discuss it in the book [ 1 ] in 1983. they gave the sufficient condition 5 that ensure the existence of the horseshoe of the model for = 1 and used numerical value method to discovered " stranfe attractor " for certain a ( for example : - 1, - 6 ) where denote the colliding coefficient of restitution and the amplitude of the ball
Guckenheimer和holmes在專著[ 1 ]中用了相當的篇幅討論了彈跳球模型，當= 1時給出了存在馬蹄的條件（ 5 ） ，對某些（ 1 ）則僅用數值方法得到在一定條件下出現「奇異吸引子」 （例如= 0 . 5 ， = 6 ） ，這里與分別表示小球的碰撞恢復系數和激振力振幅。分享友人