碼段表 的英文怎麼說
中文拼音 [mǎduànbiǎo]
碼段表
英文
code segment table-
In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank
本實驗採用了高保真pfudna聚合酶,在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段,將此片段插入克隆載體pgem - 7fz ( + ) ,經測序和序列分析表明,所擴增得到的片段含有bar基因完整的讀碼框,並且序列與genbank中發表的序列完全一致。In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中,利用pcr克隆的方法擴增出bar基因全長,並在原核表達系統中表達,鑒定表達蛋白的活性,將能夠正確編碼ppt乙酰轉移酶的bar基因片段,經過適當的修飾構建入真核表達載體。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明, pcr擴增產物中包含有完整的phya基因,該基因全長1506bp ,其中包含一段長102bp的內含子,該內含子具有真菌植酸酶基因內含子的特徵保守序列: donor序列? gtatgc , lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸,理論分子量為51 . 37kda ,其上有13個潛在的n -糖基化位點, n端19個氨基酸為信號肽序列,植酸酶活性位點序列( crvtfaqvlsrhgaryptdskgk )位於氨基酸序列的+ 71 + 93 。The serial number of pricker is composed of three sections including 17 digital codes : the first section ( 1 - 4 digits ) is the standard size of international general mode ; the second section ( 5 - 11 digits ) is penetration distance, length of functional section, pattern of barb, quantity of barbs on each cording and the distance from needlepoint to the first dent ; in the third section ( 12 - 17 digits ), except letter h representing " hengxiang " brand, the other group of digits represent some special characters of pricker
刺針的編號由三部分共17位代碼組成:第一部分( 1 - 4位)是國際通用方式的標準尺寸;第二部分( 5 - 11位)是刺距、工作段長度、鉤刺型狀、每個棱上的鉤刺數量及針尖到第一齒的距離;第三部分( 12 - 17位)除字母h代表「恆翔」牌外,其餘一組數字表示刺針的一些特殊點。This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway
測得th - nsltp序列全長為634bp ,含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架, n端的23個氨基酸組成一段信號肽序列,表明它可能和分泌有關。High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon
將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上,使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。In a word, by integrating the user " s fingerprint identification with the cryptological authentication protocol, a secure, reliable and effective approach to identifying the users in the network environment can be provided
把以指紋特徵為代表的生物識別技術與基於密碼體制的身份認證協議有機結合,向最終用戶提供一種更加安全、可靠、便捷的身份認證手段,將是成功地實現網路安全和信息系統安全的有效途徑。The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned
從陽性克隆子中提取克隆片段,經序列測定分析,結果表明,克隆片段的cdna大小為816bp ,編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank , embl , ddbj , pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索,結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ;蛋白質氨基酸序列的同源率為90 95 ,表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay
由於vgb基因啟動子不能在枯草桿菌中啟動表達,因此,根據已發表的果聚糖蔗糖酶基因( sacb )序列設計引物,從枯草桿菌wb600總dna中擴增出該基因的啟動子片段,然後將其與vgb基因編碼區及終止子序列相連,成功地組建了sacvgb融合基因。The 496 bp fragment of the orf of p22 gene and a 561 bp fragment were amplified from the genomic dna of zs strains of toxoplasma gondii. both 496 bp and 561 bp fragments were successfully cloned into the plasmid pthiohisa, b, c and pbudce 4. 1 respectively. 2
從弓形蟲zs株基因組中擴增出p22編碼基因的一長496bp ,另一長561bp的片段,並成功構建含p22編碼基因的原核質粒重組體pthiohisa , b , c / p22 ,及真核重組表達質粒pbudce4 . 1 / p22 。The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶( exonuclease , exo )順序缺失,獲得單向長度漸減重疊的系列突變體,核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps , frameplot程序分析揭示出該區域一個完整的開放閱讀框( orf )的存在,其大小為1656bps , g + c含量為70 . 3 ,編碼552個氨基酸,利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較,發現無論在核苷酸水平還是在蛋白水平上,該orf均與膽固醇氧化酶表現出同源性,而且與鏈黴菌膽固醇氧化酶同源性最高,說明該orf編碼膽固醇氧化酶基因。In charge of one of major subjects of the national eighth five - year plan, i. e. " study on shotcreting techniques of polyacrylate emulsion cement mortar for impermeability coating of pu ding rcc dam ", the achievement of which has been appraised by the ministry of electric power and evaluated as reaching national leading level. 2. in charge of the major subject aided by the committee of national natural science funds, " study on durability of high bolumes of fly ash concrete ", the first phase achievement of which has been commended by the committee. 3. in charge of one of major subjects of the national ninth five - year plan, i. e. " study on high performance concrete in marine engineering, design and construction ". 4. acting as one of editors - in - chief of " technical specification for abrasion and cavities resistance of concrete in hydraulic structures ", which is issued by the ministry of water resources. 5. by providing the repairing and strengthening schemes on seepage and cracks of the basement of nanjing lukou international airport, the air traffic opening of the airport was ensured as scheduled. 6. consulting services on techniques of high performance and abrasion - erosion resistance concrete for three - gorges project, anti - corrosion of reinforced concrete strutures for the ore port of shanghai baoshan iron and steel works, mass fly ash concrete for jiangyin yangtze river bridge and pumping concrete for nanjing yangtze river second bridge
主持國家「八五」攻關項目「普定碾壓混凝土壩上游面丙乳砂漿防滲層大面積施工工藝研究」 ,成果通過部級鑒定,評價屬國內領先水平; 2 .主持國家自然科學基金重大項目「高摻量粉煤灰混凝土的長期性能研究「 ,階段成果獲國家自然科學基金委通報表彰與獎勵; 3 .主持國家「九五」攻關項目「海工高性能混凝土成套技術研究」 ; 4 .水利部「水工混凝土抗沖磨防空蝕技術規范」的主編之一; 5 .為南京祿口國際機場地下室修漏與裂縫補提出技術方案被采納並實施,為機場如期通航作出貢獻; 6 .為三峽工程抗沖磨高性能混凝土技術、上海寶鋼礦石碼頭鋼筋混凝土防腐蝕技術、江陰長江大橋大體積粉煤灰混凝土技術、南京長江二橋泵送混凝土技術等提供咨詢建議。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術構建echistatin的融合表達基因克隆。In visual c, the completion list includes code snippets to help you easily insert predefined bodies of code into your program
在visual c #中,完成列表中包括可幫助您方便地將預定義的代碼體插入程序中的代碼段。Represents a member of a type using a literal code fragment
使用原義代碼片段表示類型成員。Displays information on the folder or code snippet file selected in the folder list
顯示有關文件夾列表中選定的文件夾或代碼段文件的信息。Select the code snippet from the complete word list
從完成單詞列表中選擇代碼段。To use code snippets through the intellisense complete word list
通過intellisense完成單詞列表使用代碼段A list of snippet categories is displayed
將會顯示代碼段類別列表。分享友人