神經氨酶 的英文怎麼說
中文拼音 [shénjīngān]
神經氨酶
英文
mucopolysaccharide-n-acetylneuraminylhydrolase-
In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。To neutralize the function of cam, the cam binding domain peptide from bovine calcineurin a subunit was chosen because the pre - experiment demonstrated that bovine calcineurin could bind to calmodulin strongely in an acidic and high calcium concentration condition which is similar to the cell wall environment. in order to increase the binding ability of the chimeric secreted calmodulin binding peptide, the plasmid containing two copies of calmodulin binding domain were constructed
它共有26個氨基酸，具有信號肽的典型特徵。為了削弱質外體cam的功能，選擇了牛腦鈣調神經磷酸酶can （ calcineurin ）的a亞基的cam結合域。預實驗結果表明，在近似於胞外環境的酸性高鈣條件下can與cam有強結合。In the present study, in order to investigate the effects of endogenous estrogen on the daergic terminals in amy and the daergic neurons in midbrain, fast cyclic voltammetry ( fcv ) was used to examine da release evoked by electrical stimulation from amy of female rats in different phases of estrus cycle, ovx rats and male rats. and tyrosine hydroxylase ( th ) immunohistochemi stry was employed to measure the numbers of daergic neurons of ventral tegmental area ( vta ) and substantia nigra ( snc ) in the rats
因此，為進一步探討大鼠內源性雌激素水平的變化對amyda能神經系統及中腦da能神經元的影響，本工作應用快速周期伏安法（ fcv ）在體監測了處于動情周期各期雌鼠、 ovx鼠和雄鼠經電刺激誘發的amyda釋放，並應用酪氨酸羥化酶（ th ）免疫組化方法測定了以上各組大鼠腹側背蓋區（ vta ） 、黑質（ snc ）的th陽性神經元數目。But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area ". to answer these questions, we observed systemically the synaptic connections among the pv - like immunoreactive neurons, fibers and terminals and the connections between dnpi - like, gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry, triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique
但是目前對新發現的囊泡膜mu轉運體一dnn樣陽性終末與帶狀區內pag樣陽性神經元之間ej關系，以及谷氨酸脫發酶（ gad ，是gaba能神經元和終末的特異性標識物）是否參與其調控作用，尚缺乏系統的形態學資料。After i. c. v. 6 - ohda, the c - fos expression of these areas induced by cold stress reduced significantly. 2 ) double staining showed that fos - like immunoreactive positive granules were observed in some vasopressin ( avp ) - immunoreactive positive neurons in paraventricular and supraoptic nuclei, as well as in some tyrosine hydroxylase ( th ) - immunoreactive positive neurons in nts and lc nuclei
（ 2 ）雙重染色顯示，寒冷應激誘導的fos樣免疫反應陽性顆粒可見于室旁核（ pvn ）和視上核（ son ）中的部分加壓素（ avp ）陽性神經元及孤束核（ nts ） 、藍斑（ lc ）中的部分酪氨酸羥化酶（ th ）陽性神經元。Glu of cns mainly comes from glutamine and phosphate activated glutaminase ( pag ) can decomposed the glutamine to glu and ammonia and is the main source of the glu of the cns, so pag is used as the special marker of glutaminergic neurons
磷酸激活的谷氨酞胺酶（ pag ）能催化谷氨酞胺水解為giu和氨，是中樞內gill的主要來源。困此pag一直被作為研究gill能神經元的特異性標識物。Newcastle disease virus ( ndv ) is grouped under the family of paramyxoviridae, it causes serious infectious disease and incurred great losses to poultry industry. f and hn protein were main glycoproteins, which affect the virulence of the virus
新城疫病毒（ newcastlediseasevirus ， ndv ）屬于副粘病毒科（ paramyxoviridae ）副粘病毒亞科（ paramyxovirinae ）腮腺炎病毒屬（ rubulavirus ）的成員，其主要結構蛋白融合蛋白（ f ）和血凝素-神經氨酸酶（ hn ）與其致病性密切相關。Sleep / waking cycle is a complex network modulation and many factors such as interleukin - 1 ( il - 1 ), tumor necrosis factor ( tnf ), growth hormone releasing hormone ( ghrh ), vasoactive intestina polypeptide ( vip ) and many conventional neurotransmitters such as serotonin ( 5 - ht ), acetylcholine ( ach ), norepinephrine ( ne ), dopamine ( da ) and gamma - aminobutyric acid ( gaba ) were involved in it. recent evidence has shown that no synthesized in neurons in several areas of the brain can induce the release of neurotransmitters. in the rat central nervous system, the anatomical distribution of nos - containing neurons is now well established, and it was reported that nos is co - localized with neurotransmitters well known for their involvement in sleep mechanisms, i. e. 5 - ht, ach, da and gaba
鄭州大學2003屆碩士畢業論文gaba受體激動劑消除no合成酶抑制劑對大鼠睡/醒周期的影響睡/醒周期的形成是一個復雜的網路調控的結果，體內許多因子都參與了這一調控網路，這些因子如白介素一1 （ il一1 ） 、腫瘤壞死因子（ tnf ） 、生長激素釋放激素（ ghrh ） 、血管活性腸膚（ vip ）以及經典的神經遞質如5一輕色氨（ 5一ht ） 、乙酞膽堿（ ach ） 、去甲腎上腺素（ ne ） 、多巴胺（ da ）和卜氨基丁酸（ gaba ）等，它們在睡眠的發生和調節中也發揮著重要作用。3. the choosing of optimum evaluating methods of immunobiologic activity : if we disposed sheep blood red cells with neuraminic acid enzyme, the e rosettes forming ratio remarkbly increased ; we improved the conventional method of brdu - elisa assay on lymphocyte proliferation induced by cona. the results showed that lymphocyte proliferation induced by pha was better than by cona. 4
3 、 gpif免疫生物活性評價方法的優化對gpife玫瑰花環實驗在不影響srbc活性的基礎上用神經氨酸酶處理，經此法處理后e玫瑰花環形成率明顯提高；對傳統的cona誘導的人外周血淋巴細胞增殖brdu - elisa實驗，進行了方法學改進。Ngf binding to trka receptors results in receptor dimerization and kinase activation. recent evidence supports that not all extracellular subdomains are responsible for receptor activation. structure based drug design for neurotrophic agonists with small molecular weight relies on knowledge of the interaction of neurotrophin with their receptors
Trka在ngf作用下發生二聚化，使胞內域中酪氨酸激酶區激活，從而使trka中酪氨酸自磷酸化，並進一步激活胞內信號轉導通路，從而實現神經營養信號傳遞。Resistance would be developed when the activities of any of these three enzymes are increased. the three insecticide target - sites are acetylcholinesterase ( ache ) for organophosphate and carbamate insecticides, voltage - gated sodium channels : knockdown resistance ( kdr or kdr - type ) for ddt & pyrethroids and y - aminobutyric acid ( gaba ) receptors for cyclodienes
殺蟲劑的作用靶標主要有：有機磷和氨基甲酸酯殺蟲劑的作用靶標?乙酰膽堿酯酶； ddt和擬除蟲菊酯類殺蟲劑的作用靶標?神經軸突鈉離子通道；環戊二烯類和吡唑類等殺蟲劑的作用靶標? -氨基丁酸（ gaba ）受體-氯離子通道復合體。Gene expression of neural related genes was identified by semi - quanti - tive rt - pcr analysis and genechip assay. 4 and 10 days after neural induction, gene expression pattern was analysed by genechips, which showed the expression of some neural stem cells and mature neurons specific and related genes, repectively. especially the expression of gabar and glutamate dehydrogenase ( gad ), which meant the induced cells could be gabanergic neurons
2 .基因晶元檢測到未分化escs 、神經幹細胞及成熟神經細胞的相關基因，並由半定量rt一pcr證實基因晶元檢測未分化細胞表達胚胎幹細胞特異基因;誘導后第4天細胞高表達神經幹細胞特異性基因;誘導后第10天細胞高表達成熟神經細胞特異性基因，且有gaba受體、谷氨酸脫梭酶（ gad ）表達，說明誘導后細胞大多為以ba能神經元。Influence of ciliary neurotrophic factor on spinal cord caspase - 3 expression in rats of different age following sciatic nerve damage
不同年齡大鼠坐骨神經損傷后睫狀神經營養因子對脊髓組織中天冬氨酸特異酶切的半胱氨酸蛋白酶3表達的影響Influence of caspase - 3 on hippocampal neuronal apoptosis following ischemia - reperfusional injury in rats
半胱氨酸天冬氨酸蛋白酶3對缺血再灌注大鼠腦海馬神經元凋亡的影響Neuronal apoptosis and caspase 3 gene expression of in vitro cultured rat hippocampal neurons of epilepsy models
體外培養大鼠海馬神經元癲癇模型中神經元凋亡及半胱氨酸天冬氨酸蛋白酶3基因的表達Injection of rats with neuraminidase, however, resulted in a major increase of urinary albumin and was associated with an increase in urinary free neuraminic acid
然而給大鼠注射神經氨酸酶卻可以使尿白蛋白大量增加，並伴有尿液游離神經氨酸增加。The neurotrophic and morphogenic activities of gdnf are mediated by its interaction with a multicomponent receptor complex formed by the ret receptor tyrosine kinase and a glycosylphosphatidylinositol ( gpi ) - anchored " accessory " receptor, gdnf family receptor alpha - 1 ( gfral )
Gdnf的神經營養作用主要是通過兩類受體亞基介導，即膠質細胞源性神經營養因子受體alpha （ gfr s ）亞基和受體酪氨酸激酶ret亞基。The fusion ( f ) protein and hemagglutinin - neuraminidase ( hn ) are the major virulence factors of the virus to which the host responds well with protective antibody production. recombinant plasmids containing the f or hn gene can induce specific immune responses against ndv. however, dna vaccination by injection or gene gun routes is rather inconvenient and costly in field applications in scaled animal farms
融合蛋白（ f ）和血凝素-神經氨酸酶蛋白（ hn ）是ndv的主要宿主保護性抗原，以f基因和hn基因為免疫原研製的dna疫苗能誘導一定程度的免疫應答，但注射免疫或基因槍免疫途徑因實際操作和成本等問題難以推廣應用，而直介面服重組質粒dna免疫效果不理想。A surface antigen hemagglutinin - neuraminidase ( hn ) gene of the ndv b95 strain, was selected to study as target gene. referred to the reported sequence, rwo primers were designed and synthesized. hn gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fraction method
為此，從澳大利亞某大學獸醫病理系引進一株ndv熱穩定性天然弱毒b95株，並選取其表面抗原血凝素?神經氨酸酶的編碼基因為目的基因，根據國外已發表的序列，設計一對引物，利用rt - pcr技術擴增出了ndvb95株hn基因。分享友人