突變克隆 的英文怎麼說

中文拼音 [tūbiànkèlōng]
突變克隆 英文
mutant clon
  • 突 : Ⅰ動詞1 （猛沖） dash forward; shoot out 2 （高於周圍） protrude; bulgeⅡ副詞（突然） abruptly; sud...
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  • 突變 : 1 （突然急劇的變化） sudden change; change suddenly; transilience; accident; saltation; revulsion...
  1. Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function

    進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。
  2. It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his

    本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。
  3. Lots of constitutive - expression fusions containing promoters of 7653r and one induced fusion by a. sinicus seed extracts were screened by shot - gun method

    酶切鑒定、亞克隆分析和序列測定結果證實phn122上含有妙和另一個發生k79r突變的肺』 。
  4. Studies of genes related to heart development in drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases. to clone and identify new genes that control the heart development, by a way of chemical mutagen, ems, we have established 1, 200 balanced - lethal lines on chromosome 2 and 3. with the screening the 330 stocks with immunochemical method using heart - specific antibody, mab. no. 3, we detected 60 lethal lines showing heart mutant phynotype

    為了克隆和鑒定控制心臟發育的新基因，本研究利用化學誘變劑甲磺酸乙酯大規模地誘變果蠅，並且建立了1200個第二和第三染色體的平衡致死系，利用心臟組織特異抗體mab . no . 3對其中330個品系進行免疫化學方法篩選，觀察到有60個致死系表現出心臟突變表型， 20個品系的心臟突變表型有待進一步證實。
  5. It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain

    通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段，將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較，結果在參比的16個ndv毒株中在該區段中除了有多個點突變外，個別毒株有堿基插入和缺失，所有以lasota株為代表的弱毒株均無6堿基的插入，而以f48e9株為首的強毒株均有此6堿基的插入，但有一個中等毒力的毒株dp沒有6堿基的插入，不過它的基因序列和lasota的幾乎相同，對于所克隆到的基因的代表性還有待確定。
  6. Molecular cloning and sequence analysis of e6 and e7 gene of human papillomavirus type

    1突變體基因的克隆及表達
  7. Mutation can also occur in cells other than germ cells - somatic mutation ? but this is likely to be apparent only when the genetically altered cell proliferates abnormally to form a large family or clone of similar cells, e. g. when a tumour forms

    突變也可發生於生殖細胞以外的細胞（體細胞突變） ，但這很可能僅表現于遺傳性改變的細胞異常增生而形成一大系或克隆的相似的細胞時，例如當一個腫瘤形成時。
  8. Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway

    摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。
  9. On the hh signal receiving cells, the function of hspg in hhn movement might be that it competes with ptc for binding of hhn, following the releasing from the disp, hhn binds to the hspg to prevent the hhn from being captured by the ptc, hence facilitate it ' s transfer to the more distantly located cells

    進一步結合運用flp frt系統，用缺損gfp蛋白的表達來標記純合的oxt突變基因克隆，針對眼睛成蟲盤的克隆分析結果表明，在oxt突變克隆中， hh下游的以蛋白的表達區域完全消失。
  10. Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %

    結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。
  11. After sds - page and densitometric scan analysis, the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 %. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %

    今士考個二目卜乙成功構建了ptxbi一hng及ptxbi一m一insulin原核表達克隆，並獲得了高效表達，經過純化得到純度人於80 %的融合蛋白，並對人胰島素突變體融合蛋白進行了初步活性測定。
  12. By artificially changing a to c at - 137 bp site upstream from transcription start point of cloned promoter, two site - mutation promoters, ipms ( 603 bp ) and ipm1 ( 900 bp ) were created

    通過pcr方法對克隆的兩個啟動子進行定點突變，使轉錄起始位點上游- 137bp處a突變為c ，得到兩個突變啟動子（ ipml 、 ipms ） 。
  13. 900 bp promoter - directed gus expression was highly induced by sa and bth, while the 603 bp promoter, whether mutated or not, did not respond to sa and bth induction, which indicated that the element in response to sa and bth lied among 575 ~ 872 bp from transcription start site

    全長900bp啟動子能夠應答sa和bth的誘導，而603bp長的啟動子無論突變與否對sa和bth均無應答，證明sa和bth的應答區域在克隆啟動子的轉錄起始位點上游575 872bp之間。
  14. The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level

    抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。
  15. Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe

    對片段進行克隆、亞克隆之後測序分析，發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性；並且三次獨立的pcr均得到相同的片段，所克隆的基因序列與choe相比，連續缺失三個堿基（ ttc ） ，即相應的氨基酸序列缺失一個氨基酸（ phe ） ，因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然突變體，命名為choew 。
  16. According to antibody - antibody ab - ab affinity and antibody - antigen ab - ag affinity, the algorithm can allot adaptively the scales of memory unit and antibody population. it is proved theoretically that the csaim is convergent with probability 1

    由於遺傳和免疫細胞在增殖中的基因突變，形成了免疫細胞的多樣性，這些細胞的不斷增殖形成無性繁殖系，無性繁殖稱為克隆。
  17. Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain, and further study and utilize mutation, deletion, insertion and substitution of csfv gene on dna molecular level

    中國豬瘟兔化弱毒（脾淋毒）全長感染性cdna的克隆和構建，可以使我們得到純粹的csfvc -株rna病毒基因組，在dna水平上研究和利用csfv的基因突變、缺失、插入和替換。
  18. Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073. marker exchange was achieved by selection on yma containing sm, km, gm, spc et al four kinds of antibiotics. mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively

    隨后，利用突變質粒pgxn217誘變慢生型大豆根瘤菌菌株gx201 ，獲得了gx201的突變體菌株gx217 ；將pgxn201的3 . 4kbecori片段與plarf3連接獲得亞克隆pgxn201cl ，然後將pgxn201cl導入突變體菌株gx217 ，構建了gx217的功能互補菌株rgx217 。
  19. After absorption, the all - frans - retinal isomerizes to a 13 - c / s configuration and br undergoes a photocycle : br570 k590 l550 m410 n520 o640 br570 bacteriohodopsin is a promising biological photoelectric material. we intent to conduct a more thermostable br by site - directed mutagensis. a point ( no. 274 t, no. 274 a turn to c g ) mutation was introduced to br gene by successive pcr technique, and cloned into puc - 19 vector

    本論文利用連續pcr的方法定點突變了br基因的一個氨基酸，突變位點選擇了br基因第273的t和274位的a ，把它們變為cg （ tyr替換為arg ） ，然後把突變的br基因克隆入嗜鹽菌表達載體pnov - r ，經測序鑒定的表達載體轉化br缺陷的嗜鹽菌，構建了tyr79 argbr突變體。
  20. We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu

    隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結果為： as基因開放讀碼框與表達載體的讀碼框正確匹配相連，但在其kringle4區相當于編碼plg的lys ~ （ 414 ）密碼子aaa的第一位堿基由a突變為g ，導致相應的氨基酸殘基突變為glu 。
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