突變基質 的英文怎麼說
中文拼音 [tūbiànjīzhí]
突變基質
英文
mutation matrix-
This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation
簡要綜述了生物技術在水稻遺傳育種中的應用，主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。Parkinson ' s disease arises from genetic and possibly neurotoxic causes that produce massive cell death of the neuromelanin - containing dopaminergic neurons of the substantia nigra
帕金森氏病的病因包括基因突變和神經毒性物質作用，導致黑質多巴胺能神經元大量的死亡。Fast algorithms of both discrete and orthonormal wavelet and wavelet packet coefficient are diagrammatized to be introduced. daubechies wavelet is applied to help to discuss the application and test on signal filtering and noise reduction with the principle and threshold implementation ; the basic principle to pickup the fault characteristics is introduced mainly about the relations between the maximum module and signal saltation point and how to characterize the saltation point with lipschitz exponent
展示了離散正交小波變換的mallat快速演算法和小波包系數分解的快速演算法；重點應用daubeches小波探討了小波變換在信號濾波去噪中的應用和實驗，闡述了其基本原理和通過閾值化處理實現濾波的具體方法；探討了用小波變換進行故障特徵提取的原理，說明了小波變換模極大值和信號突變點之間的關系以及怎樣用李氏指數來表徵突變點的性質。The seven sisters that dr de grey wishes to slaughter with sens are cell loss, apoptosis - resistance ( the tendency of cells to refuse to die when they are supposed to ), gene mutations in the cell nucleus, gene mutations in the mitochondria ( the cell ' s power - packs ), the accumulation of junk inside cells, the accumulation of junk outside cells and the accumulation of inappropriate chemical links in the material that supports cells
德?戈瑞博士想用這一策略對付的七個因素是：細胞丟失，細胞凋亡阻抑（細胞拒絕程序死亡的傾向） ，細胞核內的基因突變，線粒體（細胞的「動力倉」 ）中的基因突變，細胞內的垃圾堆積，細胞外的垃圾堆積和細胞支持性物質中不當化學鍵堆積。Monofunctional alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng ) is a widely spread environmental mutagen and carcinogen that targets dna and proteins to generate adducts. among the adducts, o6 - alkyl guanine is the predominant mutagenic lesion because of its mispairing properties, which can eventually lead to chromosomal aberrations, point mutations, and cell death. this lesion also appears to be involved in tumor initiation, particularly in gastric carcinogenesis
單功能烷化劑n -甲基- n -硝基- n -亞硝基胍（ mnng ）是一種在環境中廣泛存在的化學誘變劑和致癌劑，它能和dna及蛋白質等生物大分子形成加合物（ adduct ） ，其引起的與突變有關的主要dna損傷類型是o ~ 6 -甲基鳥嘌呤，這種損傷與腫瘤尤其是胃癌的發生密切相關。The topics include : structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease
主題包括：基因、染色體與基因組的結構和功能；來自於基因重組、突變和篩選的生物變異；族群遺傳學；運用遺傳學的方法分析蛋白質的功能，基因的調控和遺傳性疾病。Dominant negative alleles of gpil7p were produced by random pcr mutagenesis of three conserved fragments of gpi17p : 1 ) having generated the plasmid library which contains 100000 plasmids harboring gpi17 mutants then screened from 8000 clones, we got 25 strong and 3 very strong dominant negative alleles
對gpi17p可能的三個保守區進行了pcr隨機誘變，並最終獲得顯性負性等位基因： 1 ）組建了gpi17p突變體質粒庫，其所含質粒數約為100000 。Phylogenetic tree indicates the rab protein may play a role in vesicular trafficking from endoplasmic reticulum to golgi body. two tga in eo - rabl were successfully mutated to tgc by site direct pcr procedure and the truncate eo - rabl was obtained
以此重組質粒為模板進行pcr定點突變，將eo - rab1基因中前兩個tga突變為通用半胱氨酸密碼子tgc ，獲得截短型eo - rab1基因。The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。A few mutants were found on the plate containing nitrobenzene selective medium. their colony appearances have some difference from the wild strain. some evidences show that their growth characteristics on the selective medium are related to the plasmids harbored in their cells
對菌株的自發突變株的研究表明，這些菌株在硝基苯選擇培養基上的生長特性是與它們細胞中所含質粒的特性相關的。At the initial stage of planar technique, b was employed as ideal diffusion impurity in base - region of npn si planar devices because of the match of its solid - solubility and diffusion coefficient in si with those of p in emission - region, and the good shield effect of sio2 film to b. but because of the relatively large solubility ( 5 1020 / cm3 at 1000 ) and the small diffusion coefficient, the linear slowly - changed distribution of acceptor b in pn junction can not be formed, which could not cater to the requirement of high - reversal - voltage devics. thereafter b - a1 paste - layer diffusion technology and close - tube ga - diffusion technology had been developed, while the former can lead to relatively large the base - region deviation and abruptly varied region in si, which caused severe decentralization of current amplification parameter, bad thermal stability and high tr ; the latter needed the relatively difficult pack technique, with poor repeatability, high rejection ratio, and poor diffusion quality and productio n efficiency
在平面工藝初期，由於b在硅中的固溶度、擴散系數與n型發射區的磷相匹配， sio _ 2對其又有良好的掩蔽作用，早被選為npn硅平面器件的理想基區擴散源，但b在硅中的固溶度大（ 1000時達到5 10 ~ （ 20 ） ，擴散系數小， b在硅中的雜質分佈不易形成pn結中雜質的線性緩變分佈，導致器件不能滿足高反壓的要求，隨之又出現了硼鋁塗層擴散工藝和閉管擴鎵工藝，前者會引起較大的基區偏差，雜質在硅內存在突變區域，導致放大系數分散嚴重，下降時間t _ f值較高，熱穩定性差；後者需要難度較大的真空封管技術，工藝重復性差，報廢率高，在擴散質量、生產效率諸方面均不能令人滿意。Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa
將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。The structure system of this paper is : first to introduce the historical background and relevant definition of decision - making ; then to present the theory frame of error - eliminating and pave the way to follow - up chapters ; the following chapter is core content of this paper : first of all, the relevant definition and characteristics of change of time and space based fuzzy error system are put forward, and then detailed discussions on time delay, mutation, interaction, error category, extreme value of error system and transformation words of error system are conducted
本論文結構體系為：首先系統介紹了決策相關的歷史沿革以及相關的定義和理論；其次介紹了消錯學的理論框架，為后續章節的論述作好鋪墊；再者是本文的的核心部分：首先引入基於時空變化的模糊錯誤系統的定義及相關的性質，而後在各節中分別探討了模糊錯誤系統的時滯，熵變，突變；以及決策錯誤種類分析，錯誤的相互作用，模糊錯誤系統的極值和模糊錯誤轉化詞的應用。The wavelet method used in economic forecast depends on its " mathematics microscope " property. it does the layer analysis and forecast to indicators. it can improve forecast precision, what ' s more it can search and express the structural feature of data such as development cycle, second cycle, especially to some sudden change data which will provide effective and reliable warranty to the complexity and violent fluctuant data indicators in enterprise
基於小波進行經濟預測的方法依靠其「數學顯微鏡」的特性，對待分析的預測指標進行逐層分析和預測，在提高預測精度的基礎上，能對分析數據對象的結構特徵進行挖掘，分析數據特徵如發展主周期、次周期等，尤其對具有突變性質的數據具有很好的表徵分析能力，這對于企業中復雜的變動劇烈的數據指標的預測能提供有效可靠的保證。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。As cells turn cancerous, however, the genes that code for one or the other of these proteins become mutated or otherwise inactivated
不過，當細胞癌化后，負責這兩種蛋白質之一的基因不是出現突變，就是失去活性。In this thesis the lbsp identification medium screening techniques was used. only after one cycle, large quantities no - function recombination plamid and enzyme activity increasing 20 times recombination plasmid were obtained
本研究用lbsp鑒別培養基直接篩選技術，僅一個循環就獲得了大量無酶活的重組質粒和酶活提高了20倍的突變基因。We are finding evidence that the mutation underlying huntington ' s is double - barreled : it not only encodes an abnormal protein that appears to be toxic to nerve cells, but the faulty protein can no longer prompt production of a key growth factor, starving a particular part of the brain
我們有證據顯示，杭丁頓氏癥突變基因的作用乃是雙管齊下，它不只製造出對神經細胞似乎有毒的不正常蛋白，同時該缺陷蛋白也無法促使某個重要生長因子的生成，造成腦中某個特定部位少了營養物質的滋潤。分享友人