等位基因酶 的英文怎麼說
中文拼音 [děngwèijīyīn]
等位基因酶
英文
allelozyme-
Table 2. the frequencies of ace genotype and allele
表2 .各組血管緊張素轉換酶基因型和等位基因頻率的比較In this experiment, seedlings of arabidopsis thaliana ( col ) were observed after being treated by verlicillium dahliae ( vd - toxin ), exogenous salicylic acid ( sa ), nitric oxide donor ( snp ) and nitric oxide synthase inhibitor ( nna ), then we investigated the changes of endogenous h2o2 content, the activity of the antioxidant enzymes catalase ( cat, ec : 1. 11. 1. 6 ) and ascorbate peroxidase ( apx, ec : 1. 11. 1. 11 ) and mrna levels of cat3 in different stress conditions, we also identified the localizations of h2o2 and no accumulated in the leaves of arabidopsis
本實驗研究了棉花黃萎病菌?大麗輪枝菌毒素（ vd - toxin ）與擬南芥幼苗互作反應中外源sa 、 no供體snp 、 no合酶抑制劑nna等不同處理對擬南芥幼苗h _ 2o _ 2含量、 cat和apx活性及cat基因mrna表達量的影響，並對no 、 h _ 2o _ 2的積累部位進行染色檢測。The results of issr analysis is ppb = 66. 67 %, h = 0. 2089, i = 0. 3172. genetic diversity of issr is higher than that of allozymes. genetic diversity of d. pleiantha is higher than d. versipelis
六角蓮的遺傳多樣性高於於八角蓮，等位酶的多態位點比率p為55 . 6 ， issr的多態位點百分率均為70 . 83 ，基因多樣性為0 . 2383 ， shannon多樣性指數為0 . 3576 。Found the phenomenon observed in the nematode caenorhabditis elegans for the first time in 1998, consequently similar processes have been described for drosophila melanogaster, trypanosome, mammals including humans. the mechanism is that sirnas is the mediator, which can induce the risc to the target mrna and degrade it. recently there was great progress in the specific gene therapy and anti - virus, and rnai has been a focus of rna molecular therapy
自1998年fire等在研究線蟲時首次發現以來，相繼在果蠅、渦蟲、錐蟲、小鼠及哺乳動物細胞中發現rnai現象。一般認為： rnai第四軍醫大學碩士學位論文效應作用機制是sirnaskmallinterferinguaduplex ）作為中介分于，引導risc （ rnaiinducingsuppresscomplex ）至靶基因m洲a處，隨后核酸內切酶將之降解。The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea, which characterized by low temperature, high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate, self - supporting stocks of krill. 2 genetic diversity and differentiation of p. latifrons specimens of p. latifrons were collected from the east china sea and the south china sea. the zymogram phenotypes of aspartate aminotransferase ( e. c. 2. 6. 1. 1, aat ), alkaline phosphatase ( e. c. 3. 1. 3. 1, alp ), a - amylase ( a - amy ), r - amylase ( r - amy ), esterase ( est ), lactate dehydrogenase ( ldh ), raalate dehydrogenase ( mdh ), malic enzyme ( me ), and phosphoglweoisomerase ( pgi ) were scored
（二）寬額假磷蝦遺傳多樣性和遺傳分化研究1 .本文對東海外海和南海2個站位寬額假磷蝦群體進行了分析，在檢測的9個酶系統中，共檢測到11個酶位點:天冬氨酸轉氨酶（ l個位點， 2個等位基因） ，堿性磷酸酶（ 2個位點， a加了和a加2各有2個等位基因） ， r澱粉酶（ l個位點， 2個等位基因） ，醋酶（ 2個位點， es巧和est7各有2個等位基因） ，蘋果酸脫氫酶（ l個位點， 3個等位基因） ，蘋果酸酶（ l個位點， 2個等位基因） ，乳酸脫氫酶（ l個位點， 4個等位基因） ，磷酸葡萄糖轉氨酶（ l個位點， 3個等位基因） ; a澱粉酶為單態。The bvdv nadl strain is cytopathogenic. searching fromgenbank several cp strains " sequence of e2 gene about deer - n21, sh9, newyork - i and so on were found. according to the homologous sequence they were designed and synthesised a pair of primers by the biology software primer 5 and added bal i and nco i site to the 5 " end which can be used for the application of e2 gene and subcloning
從genbank中搜尋出deer - n _ （ 21 ） 、 sh9 、 newyork -等cp型毒株的e _ 2基因序列，根據其同源性用生物學軟體primer5設計引物，並在引物的兩端加入bal和nco兩個酶切位點，酶切位點的存在易於對e _ 2基因進行克隆操作。Restriction enzymes and dna probes are used to determine the exact sequence, but it is possible to get a rough estimate by analyzing the frequency of recombination between the alleles of linked genes
雖然可以利用限制性內切酶和dna探針精確的分析出特定序列，但是在分析連鎖基因等位基因間的重組序列時，會出現不準確的估計。In this paper, with the helps of the ordinary ecological site - study techniques, electrophoresis and the multivariate analyses, from both the levels of morphological and allozymic variation, we studied the differentiation patterns of 29 morphological characters and 6 allozymes of 97 individuals from 7 populations of euonymus chloranthoides yang, an endangered plant species which is endemic to mt. jinyun of chongqing. we also studied the relationship between such differentiation and its environment factors
本文以處于瀕危狀態的縉雲衛矛為研究對象，在測定了各種群的生存環境因子基礎上，從形態和等位酶兩個層次採用多種數量分析方法對縉雲衛矛7個種群97個個體的29項形態指標及6種等位酶反映出的生態遺傳分化及其與環境的關系進行了研究。Abstract : the polymorphism of angiotensinogen gene at position 174 was studied in 90 cases of essential hypertension patients and 109 controls by pcr, restriction enzyme analysis and electrophoresis methods. the results showed the distribution of genetypes in hypertension group was significantly different from that of controls group. this suggested there is a correlation between the variant of agt174 and hypertension
摘要本文採用pcr 、限制性酶切和電泳分型等方法，分別對90例原發性高血壓患者和109例正常人血管緊張素原基因多態位點agt174進行了檢測，結果表明，高血壓組中三種基因型的分佈與對照組顯著不同，提高該位點變異與原發性高血壓的發生相關。The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work
高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。Systematic cluster analysis was carried out on hu sheep in china in comparison with the same data of 9 asia sheep populations and 5 european sheep ( breeds in japan ) populations. 15 populations can be clustered in terms of gene frequency of 10 loci and 33 allele in blood enzyme and other protein variations
摘要以中國湖羊為研究對象，搜集國內外9個亞洲綿羊群體和5個在日本的歐洲綿羊群體的相同資料作為對照，根據控制血液酶和其他蛋白質變異的10個基因座位共計33個等位基因的頻率，進行系統聚類分析。Abstract : biological invasions are a continuous feature of a non - equilibrium world, ever more so as a result of accidental and deliberate introductions by mankind. while many of these introductions are apparently harmless, others have significant consequences for organisms native to the invaded range, and entire communities may be affected. here we provide a survey of common models of range expansion, and outline the consequences these models have for patterns in genetic diversity and population structure. we describe how patterns of genetic diversity at a range of markers can be used to infer invasion routes, and to reveal the roles of selection and drift in shaping population genetic patterns that accompany range expansion. we summarise a growing range of population genetic techniques that allow large changes in population size ( bottlenecks and population expansions ) to be inferred over a range of timescales. finally, we illustrate some of the approaches described using data for a suite of invasions by oak gallwasps ( hymenoptera, cynipidae, cynipini ) in europe. we show that over timescales ranging from 500 10000 years, allele frequency data for polymorphic allozymes reveal ( a ) a consistent loss of genetic diversity along invasion routes, confirming the role of glacial refugia as centres of genetic diversity over these timescales, and ( b ) that populations in the invaded range are more subdivided genetically than those in the native range of each species. this spatial variation in population structure may be the result of variation in the patchiness of resources exploited by gallwasps, particularly host oak plants
文摘:生物入侵是不均衡世界的一個永恆話題，尤其是當人類有意或無意地引入物種后.很多引入顯然是無害的，但另外一些則有著嚴重的後果，會給入侵地的生物以至於整個生物群落造成影響.本文總結了分佈區擴張的常見模式，概述了它們對遺傳多樣性和種群結構式樣所造成的影響.描述了如何根據以一批遺傳標記所得到的遺傳多樣性式樣來推斷入侵途徑，來揭示伴隨擴張選擇和漂變在形成種群遺傳樣式中的作用.本文對日益增多的群體遺傳學方法進行了總結，這些技術可以用來在不同的時間尺度上推斷種群規模所發生的巨大變化（瓶頸效應及種群擴張） .最後，我們以歐洲櫟癭蜂（膜翅目，癭蜂科，癭蜂族）一系列入侵的數據為例對一些方法進行了說明.從500 10000年的時間尺度上，多態的等位酶位點上等位基因頻率的數據表明: 1 ）遺傳多樣性沿入侵路線呈不斷下降的趨勢，支持了冰河期避難所作為遺傳多樣性中心的作用; 2 ）入侵地區的種群與該物種原產地的種群相比，遺傳上的分化更為強烈.這種種群結構在空間上的變異可能是被櫟癭蜂開發的資源尤其是櫟樹寄主在斑塊上出現變異的反映Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。This research use high sensitive, special in situ pcr technology to determine the material which is treated by the paraffin wax and formalin fixation of different time, by detecting the genotype of mn blood group of the organizes slices. at the same time, we study the research of main influence factors, such as protease digestion, etc. we hope to set up a kind of steady, practical methods to detect the genotype of the material treated by paraffin wax and formlin, offer a kind of new detecting means for the forensic appraises and iditificition with individual material evidence
本研究應用靈敏度高、特異性好的原位pcr技術，測定福爾馬林固定不同時間的石蠟切片組織mn血型的基因型，並對蛋白酶消化時間等主要影響因素進行了初步的研究，以建立一種穩定、實用的檢測石蠟組織切片dna遺傳標記的方法，為法醫物證鑒定和個人識別提供一種新的檢測手段。In the first part of the present work, the expression changes of angiotensinogen ( agt ) and angiotensin - converting enzyme ( ace ) as well as its time course characteristics were investigated by rt - pcr, in situ hybridization and western blotting. we also prepared the polyclonal antiserum against agt for the following work
故本工作應用rt - pcr和原位雜交、 western印跡分析等方法對模擬失重大鼠不同部位動脈血管血管緊張素原（ angiotensinogen ， agt ）及血管緊張素轉化酶（ angiotensin - convertingenzyme ， ace ）基因表達變化的時程特徵進行了觀察，並為后續工作制備了抗agt多克隆抗血清。In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。Application of rapid detection for rifampin resistance in clinical isolates of mycobacterium tuberculosis by phage amplified biologically assay
等位基因特異性多重聚合酶鏈反應方法用於快速檢測結核分枝桿菌利福平耐藥株This group of mutant alleles turned out to be a gene encoding a xylosyltransferase, which is an enzyme transfers the xylose from udp - xylose to the serine of the protein core
這一組突變體等位基因編碼一種木糖轉移酶- oxt ，這種轉移酶能夠將udp -木糖中的木糖轉移到核。Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr
從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。Genes that control apoptosis are included bel - 2 gene family and caspase family
控制細胞凋亡的基因包括bcl - 2等位基因家族和促進細胞凋亡的蛋白激酶caspase家族。分享友人