等分子量蛋白 的英文怎麼說

中文拼音 [děngfēnziliángdànbái]
等分子量蛋白 英文
homomolecular preteins
  • 等 : Ⅰ量詞1 （等級） class; grade; rank 2 （種; 類） kind; sort; type Ⅱ形容詞（程度或數量上相同） equa...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 量 : 量動1. （度量） measure 2. （估量） estimate; size up
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Fusion protein expression system can overcome those problem, increased the yield of yield of recombinant protein in e. coli. this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis, improved folding, and efficient mrna translation. fusion protein also make the detection and purification easy, is a good strategies for achieving high - level expression of genes in escherichia coli

    小分子量異源蛋自在人腸桿菌的表達受mrna不穩定、翻譯起始效率低、易被蛋自酶降解等因素的干擾，較難獲得高效表達，通過與已高表達的蛋自融合表達可以克服以上問題，可以使大多數蛋白獲得高效表達。
  2. By race pcr, amplified products were obtained with all the gene - specific primers and the adaptor primers

    由氨基酸序列推斷的ejo基因編碼蛋白的等電點為6 18 ，分子量為28
  3. Layered and pillared material are a kind of multifunctional material which were developed in recent years, much attention has been paid to this kind of material for its application in ion - exchange catalysts solid state proton conductivity, nonlinear optics and physic. a lot of literature have reported the intercalation behavior of a - zirconium phosphate ( abbreviated as a - zrp ), different guest molecules inserted into a - zrp have been studied in detail, those guest molecules include amine, alcohok amino acid protein, enzyme coornadiate compound and coronal compound. the intercalation guest is restricted by their size and basicity

    層柱材料是近年來發展起來的一類多功能材料，由於其在離子交換、催化、固態質子導體、非線性光學以及醫學等方面的廣泛應用而受到國內外研究者的重視，大量文獻報道了-磷酸氫鋯zr （ hpo _ 4 ） _ 2 ? h _ 2o （ - zirconiumphosphate ，縮寫為- zrp ）的超分子插層化合物及插層性能，其中對不同的客體分子對磷酸鋯的嵌入做了詳細的報道，客體分子的種類包括氨、醇、氨基酸、蛋白質、酶、配合物、冠狀化合物等。
  4. A page gel, stained by the petiodic acid - schiff ' s method to reveal glycoproteins, further displayed that the bindng - protein was a glycoprotein belonging to lectin, which contained 17. 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method. peptide mapping was comparable to the reported protein in protein bank. the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase, aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b, glutamyl - trna reductase, histidyl - trna synthetase

    連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 「 . skda ，由二個相同的亞基組成，亞基分子量為」 . ikda ，等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白，其中糖含量為17 . 44 % ，蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素
  5. Rubber tree ( hevea brasiliensis ) is an important economic woody - crop in tropical areas. its latex is the unique source of crude rubber used in current industry. because of its special and important use, the rubber tree has been extensively planted in tropical areas. increase production is always the main target in rubber tree cultivation. since the ethrel was applied in increasing latex production in 1968 for the first time as a chemical stimulant, not only the latex production had been increased largely, but also a new set of rubber tapping system had been established, leading to a series of economic benefit. owing to ethrel " s extensive application, its side effects had been found more and more obviously, such as tapping dry, speeding up senescence, shortening the life span of rubber tree etc. in order to overcome the side effects and increase production more availably, for a long time, people had carried out lots of research work on cell level, membrane level, physiology and biochemistry of laticifer contents. but the mech anism why ethrel increased latex production was not yet understood completely. this study had cloned the ethylene receptor gene ( efrl ) from rubber tree, and researched the relationship between etrl expression in laticifers and ethrel stimulation on transcription level and protein translation level. the results were as follows : 1

    但是，由於乙烯利應用的普及，乙烯利刺激割膠引起橡膠樹發生死皮病及加速膠樹衰老，縮短膠樹壽命等副作用也越來越明顯。為了克服這些副作用，使乙烯利能更有效地刺激增產，長期以來，人們在細胞水平、膜水平和乳管細胞內含物的生理生化層面上進行了大量的研究，但仍未完全了解乙烯對膠樹的作用機制。本研究從分子水平入手，克隆橡膠樹的乙烯受體基因（ etr1 ） ，並在轉錄水平和蛋白質翻譯水平上研究etr1基因在乳管細胞中的表達及與乙烯刺激的關系，取得了以下結果： 1
  6. The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male

    Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。
  7. Dye probes and their recent applications in quantitative analysis of protein and in the studies of interactions of the dye probes with protein / dna in bio - analysis were reviewed

    摘要評述近年來染料探針在生物分析中用於蛋白質的定量測定及蛋白質、核酸與小分子相互作用等方面的研究進展。
  8. Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively

    Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
  9. Efficiency : contain several minerals and micro elements, active spring, rose hips oil, micro molecule polypeptide and ceramide, instantly and constantly supply moisture, nutrition can effectively penetrate into deep skin, accelerate the formulation of collagen, activate cells regeneration, remove dry and fine lines, smoothen and nourish skin, balance water and oil and constantly moisturize skin

    功效：富含多種礦物成份和微量元素、活泉水、玫瑰果油、小分子多肽、神經酰胺等，迅速持久補充肌膚水份，營養成份有效滲透肌膚內層，加速膠原蛋白的合成、激活細胞再生、減少干紋和細紋的產生，平滑、滋養肌膚；同時給肌膚長效保濕，令肌膚水油平衡、持久滋潤。
  10. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  11. Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years. however, a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly. therefore, it is a challenging task to develop strategies for improving the detection limit sensitivity of spr. in this paper, authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex. it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity. this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex

    將鏈霉親和素-生物素系統用於表面等離子體共振免疫傳感的信號放大，實時檢測了人免疫球蛋白g higg的蛋白濃度。發生免疫反應的傳感片和生物素化抗體反應后，傳感片表面的一層生物素分子隨后與鏈霉親和素-生物素化抗體復合物中的鏈霉親和素的活性位點發生親和反應，從而使傳感片表面特異健合的物質質量顯著增加，大大提高了免疫檢測的靈敏度和檢測限。免疫反應經放大后，可檢測0 . 00510g ml濃度區間內的higg 。
  12. Skin sensitization test in rats showed that application of the ccx leaching solution caused no obvious skin reddening, regional edema, or skin necrosis

    結果雞羽根在浸出液中釋放的主要物質為中等分子量的肽類物質，大分子蛋白釋放不多。
  13. Nonspecific lipid transfer proteins ( nsltps ) are small, soluble, basic proteins that can bind and catalyze transfer of lipids in vitro, however, we do not know their functions in vivo

    非特異性脂轉運蛋白是一類分子量較小的可溶性堿性蛋白質，許多研究表明在體外它能結合併跨膜運輸磷脂、糖脂和脂肪酸等物質。
  14. These proteins involved in various cellular functions including carbohydrate transport and metabolism, energy production and conversion, inorganic ion transport and metabolism, amino acid transport and metabolism, posttranslational modification, protein turnover, chaperones, function unknown and not in cog most of the proteins identified are involved in energy production and conversion, carbohydrate and amino acid transport and metabolism, especially in energy production and conversion

    這些蛋白質在細胞中行使不同的功能，主要包括: 1 .糖類轉運與代謝: 2 .能量的產生與轉化; 3 .無機離子轉運與代謝: 4 .氨基酸轉運與代謝; 5 .翻譯后修飾，蛋白代謝，分子伴侶: 6 .一些未知功能的蛋白等。在鑒定的13個蛋白中大多數是與糖類和氨基酸轉運及代謝和能量的產生與轉化相關。
  15. These essential component, hemagglutination, toxicity, immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein were studied. the range of membrane protein mw is from 14kd to 100kd by means of sodium dodecyl sulfate - polyacrylamide gel electrophoresis, and molecular weights of the protective antigens were 36kd and 64kd by sds - page

    本研究以分離豬肺炎支原體野毒株（ xy - 1株）的膜蛋白為試驗材料，系統地研究了膜蛋白的分子量范圍、血凝性、毒性及免疫原性等。
  16. The results of sequencing and structural analysis showed that the gene, named crylaa14 by international nomenclature committee of bt 5 - endotoxin, was 3552 bp and the open reading frame encoded a 133. 7 kda protein with 1183 amino acids

    序列測定結果表明該基因編碼區為3552bps ，編碼1183個氨基酸，分子量為133 . 7kda ，等電點為pi4 . 755 。該基因序列已在genbank注冊， accessionnumber為ay197341 ，並被國際bt殺蟲晶體蛋白基因命名委員會正式命名為cry1aa14 。
  17. Sequence analysis showed that the full length of this cdna which encodes 364 amino acids is 1398bp. it has 71 % and 69 % identities to lycopersicon esculentum and pisum sativum respectively in amino acid level. this gene is a membrane protein which has one signal peptide, seven transmembrane helices, three n - glycosylation sites and one o - glycosylation site

    序列分析表明，該基因的cdna全長為1398bp ，開放閱讀框為1095bp ，編碼一個364個氨基酸的多肽，與番茄和豌豆中的該家族基因分別具有71和69的氨基酸同源性，是一種膜蛋白，具有1個信號肽序列， 7個跨膜螺旋， 3個n ?糖基化位點和1個o ?糖基化位點，分子量大約為39 . 174kd ，等電點為8 . 07 。
  18. It is at chromosome 8p22. the molecular weight of sh2a protein is 57, 726da, pi is 8. 09

    Alone人定位於8p22 ，編碼的蛋白質分子量57 ， 726道爾頓，等電點為8
  19. Rab proteins are a family of small molecular weight gtpase of ras superfmaily with characteristic molecular weight about 22 - 25 kd. rab gtpases are expressed in almost all eukaryotic cells, ranging from yeast to drosophila and to human. they are ubiquitously or tissue - specifically expressed and function in all of cellular aspects

    Rab蛋白是屬于ras蛋白超家族的小g蛋白（ smallg - proteinorsmallgtpase ） ，分子量大多在22 - 25kda之間，表達于從酵母、果蠅、小鼠到人類等各種真核生物中，發揮著各種各樣的功能；其在酵母中稱為ypt sec4p在高等真核生物中稱為rab 。
  20. Studied the pl molecular weight histidine of catalytic group of mlap and the influence of zn2 + ca2 + and edta to enzyme activity for validation the results of bioinformatics analysis and homology modeling. the cold - adaptation is validated by the catalysis of mlap

    為了驗證堿性蛋白酶的生物信息學分析結果和模建的結果，我們對其等電點、分子量、催化性基團組氨酸以及鋅離子、鈣離子和edta的影響進行了實驗分析。
分享友人
分享到 Line

分享到臉書