Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control
選取單個菌
落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌
落，即陽性菌
落，再以陽性菌
落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素
篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，
篩選氨芐青霉素抗性菌
落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle
首先用粗略制備的原生質體經紫外誘變
篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹
篩選平板，恆溫培養2 3d ；按透明圈大小進行預初
篩，挑選徑圈比大的菌
落接斜面，恆溫培養3d ；按一株一瓶的方式進行初
篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復
篩；挑取酶活大且穩定的1 2株進入下一代誘變
篩選。
A stripping uint with the cross - flow fan was developed, aimed to reduce the grain losses at the rear of the stripping drum reduce the blocking to the air outlet sifter and diffuse dust. this dissertation gives overall scheme of the rig and had mad experimental study
針對非氣吸式割前摘脫裝置在收獲作物時，出風口
篩面發生堵塞使裝置內流場變差，導致裝置的
落粒損失率過大；以及出風口處煙塵四散，影響了駕駛員的視野和危害工作人員的身心健康等生產中存在的問題。
Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced
首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初
篩得到29株菌
落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
The series equipment versatillty, its performance had achieved the international advanced level, is at present is most effective, the practical reliable crushed stone machine, is suitable specially for the manufacture grinding compound, fireproof the matreial, the cement, the quartz sand, the emery, the stove are cut broken glass the power, the copper ore, the concrete aggregate and so on many kinds of, the crisp materials on the control granulated substance machine energy conserva tion 50 %, is in the present world system qranulated substance equipment
物料由機器上部垂直
落入高速旋轉的葉輪內，在高速離心力的作用下，與另一部分以傘狀形式分流在葉輪四周的物料產生高速撞擊與粉碎，物料在互相撞擊后，又會在葉輪和機殼之間以物料形成渦流多次的互相撞擊、摩擦而粉碎，從下部直通排出，形成閉路多次循環，由
篩分設備控制達到所要求的成品粒度。
After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated
重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上
篩選陽性菌
落，然後用不同濃度的g418 ? ypd板
篩選多拷貝插入單菌
落。
We educed the principal factors that led the young seedling of lilium tsingtauense population to die were imputed to the serious drought during l ~ 2years old, and the deficiency of soiar light in the communities, as well as in weaker intrinsic resistance to unthvorable environmental conditions
環境
篩分析得出，導致青島百合種群幼齡個體死亡的內在原因是其較弱的抗逆性和適應能力，外在原因是嚴重的乾旱和水澇、群
落內的光照不足及外界干擾。
When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a, 109 candidates interacting with nifa had been selected by testing for the expression of the his3, ade2 and lacz reporter genes
誘餌質粒和文庫質粒共轉化釀酒酵母（ saccharomycescerevisiae ） pj69 - 4a ，通過檢測報告基因his3 、 ade2及lacz的表達進行
篩選，初
篩得到109個陽性酵母菌
落。
The light wind strewed it with beautiful shadows of clouds and trees.
清風在其上
篩落下雲朵和樹木的美麗的影子。
Principle of operationthe ring hammer granular crusher makes use of impact hitting hammer rings cross mounted on the rotating rotor to crush materiel. after first crushed by impact force of hammer rings, the materiel is then pressed, sheared and ground between crushing plate and ringhammers to the required granular product which flows off the screen, and those uncrushed such s tramp iron, wood pieces, stone etc will be brought into the tramp iron trap. this crusher features little blast wind, less vibration andlong, service life of vulnerable
工作原理環錘式破碎機利用旋轉的轉子帶動環錘對物料進行沖擊破碎，被沖擊后的物料又在環錘和破碎板、
篩板之間受到壓縮、剪切、碾磨作用，使物料達到所需的粒度，被破碎后的物料經
篩板的柵孔
落下，不能被破碎的鐵塊、木塊、石塊等經撥料板撥進除鐵室.本機具有鼓風量小、振動值小，環錘等易損件壽命長等特點。
After crushed and granulated, they ll pass through the vibration sieve to be graded, and granules which meet requirement can be obtained. powder after the sieve will return to the vibrastion hopper to be granulated circularly
料片
落入破碎整粒機整粒后進入振動
篩過
篩分級，得到符合要求的顆粒產品，
篩下軸粉返回振動料斗循環制粒。
These controlling programmes are like a perfect sieve, they distinguish bad and good people, by means of this they also specify their next incarnation development, either to higher society in the 5th dimension or to green planets in this galaxy in the 3rd dimension ( there are six of them ) there are now primitive tribal nature societies, picking fruits ( they do not grow any plants or breed domestic animals, they do not drink milk )
這些控製程序像一個完美的
篩子，他們區別壞人和好人，藉由這他們也指定他們的下個賦與化身的發展，要麼進入第5維更高社會要麼進入這銀河中第三維綠色行星（它們有六個）現在有原始狀態部
落社會，採摘水果（他們不種植任何植物或繁殖家畜，他們不喝牛奶） 。
There was freckled places on the ground where the light sifted down through the leaves, and the freckled places swapped about a little, showing there was a little breeze up there
有些地方，陽光透過樹葉，往下
篩落，留下了地上幾處斑斑點點亮色。每當這些地方亮色搖曳，便知道有微風吹拂過。
Analyse the research of distributing of microbe community and the tendency of the change, disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism, to research the law of change that the microbe community have in composting process, we can get effective and rapid information to filtrate the microorganism during composting process, then accelerate the development of compost technology
摘要對堆肥微生物種群分佈及其動態變化的研究進行了分析，論述了分子生物技術中的變性梯度凝膠電泳和末端標記限制性片段長度多態性的原理和特點，以及用於研究堆肥微生物的群
落結構演變規律，為分析和
篩選堆肥中的微生物提供更加有效、快速的信息，促進堆肥技術的發展。
Smith noticed that while jordan had been wildly competitive before he had been cut, after the cut he seemed even more competitive than ever, as if determined that it would never happen again
史密斯發現在喬丹被
篩選掉之前是不甘於人後的，而在
落選之後他的好勝心似乎有過之而不及，好像決心不讓歷史再次重演。
The crushable constituents of the feed material are crushed between the crushing discs and the pressure rolls and pass subsequently the screening openings
物料的可碎部分在破碎盤和壓力軋輥之間被破碎，然後透過輥軸
篩落下。
But joab and abishai pursued abner, and as the sun was setting, they came to the hill of ammah, near giah on the way to the wasteland of gibeon
約押和亞比
篩追趕押尼珥，日
落的時候，到了通基遍曠野的路旁，基亞對面的亞瑪山。
Joab also and abishai pursued after abner : and the sun went down when they were come to the hill of ammah, that lieth before giah by the way of the wilderness of gibeon
24約押和亞比
篩追趕押尼珥，日
落的時候，到了通基遍曠野的路旁，基亞對面的亞瑪山。
But joab and abishai pursued abner, and when the sun was going down, they came to the hill of ammah, which is in front of giah by the way of the wilderness of gibeon
撒下2 : 24約押和亞比篩追趕押尼珥、日落的時候、到了通基遍曠野的路旁、基亞對面的亞瑪山。
