篩選性質 的英文怎麼說

中文拼音 [shāixuǎnxìngzhí]
篩選性質 英文
sifting property
  • 篩 : 名詞[書面語] （植物名） sedge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 篩選 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; ...
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. In the research field of food preservation, an intense interest for scientists is focused on the isolation of antimicrobial substances from microbial metabolites. natural biopreservatives derived from traditional fermented foods are generally recognized as safe. in this paper, one strain screened for its safety and broad antimicrobial spectrum and its antimicrobial substance - - bacteriocin are studied in detail

    本文從傳統的發酵食品中篩選出一株安全、具有廣譜抗菌活性的芽孢桿菌，並對其抗菌活性物質細菌素進行分析，為微生物天然生物防腐劑的開發以及在食品中的應用提供理論依據。
  3. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  4. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  5. A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

    Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. Then the pcr product was purified, ligated into pgem - t vector by ta cloning

    篩選陽性克隆，測序，大量制備序列完全正確的質粒。
  8. The main results obtained from this work are as follows : 1. the penicillium. sp ds9701, a strain of degrading phb, was mutagenized by uv treatment

    本項工作主要是篩選能高效降解phb的突變株，並對其進行酶學性質的研究，主要結果如下： 1
  9. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。
  10. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  11. The profile of microbial community structures in different deep sea sediments was evaluated and the interaction between microorganisms and environment was analyzed by culture - independent molecular phylogenetic methods. psychrophilic and psychro - tolerant bacteria were cultured and used for screening the cold - active enzymes

    此外，本文還對採集自太平洋的深海沉積物和南極、北極區域的樣品進行了低溫微生物的分離培養及低溫酶的篩選與性質分析。
  12. The biological characteristics of mycelia from phellinus igniarius and culture media were studied. two kinds of culture media were suitable for the growth of mycelia. the result indicated that the culture medium with potato as nitrogen source and saccharose as carbon source was suitable for collecting mycelia, and the culture medium with peptone as nitrogen source and solvable amylum as carbon source was suitable for conservation

    為了最大限度地保存菌種的活力，以提高菌絲體的質量及菌絲體內活性成分的累積，本文通過對比研究，進一步對其生長基質進行篩選，明確了兩種適于桑黃菌絲生長的固體培養基：以馬鈴薯為氮源、蔗糖為碳源的培養基較適用於菌絲收集，以蛋白腖為氮源、可溶性澱粉為碳源的培養基較適用於菌種的保藏。
  13. ( 4 ) transformation of mdmv - cp gene mdmv - cp gene was successfully into r18. w18 and 501 in 2001 fall, each material being bombed 20, 15, and 10 plates. 213 bastar ( effective constitution ppt ) resistant plants were obtained of which r18, w18 and 501 resistant plants counted 103, 90, and 20 plants respectively. molecular test was undergoing

    O ） mdmvcp因的邀傳轉化於2001年秋用基因槍將mdmvcp基因巾35scpbar質粒）導入、 w18 、 501三個優良自交系，利用已建立好的遺傳轉化體系，經抗性篩選、分化、壯苗等步驟，已得到抗bastar （有效成分ppt ）植株213棵，其中r18103株、 w1890株、 50120株。
  14. The high binding affinity and selectivityin conjunction with the polymers ' physical robustness positions molecular imprinted polymers ( mips ) as candidates for use as preliminary screens in drug discovery

    聚合物的高親和力、高選擇性及物理性質穩定特點使得分子印跡聚合物可用於藥物開發中的初篩。
  15. The topics include : structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease

    主題包括：基因、染色體與基因組的結構和功能；來自於基因重組、突變和篩選的生物變異；族群遺傳學；運用遺傳學的方法分析蛋白質的功能，基因的調控和遺傳性疾病。
  16. The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli

    從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e
  17. Metallophalocyanines ( mpcs ) are a kind of centrosymmetric planar organo - metallic molecules with an extensively delocalized two dimensional conjugated - electron system which show a relatively large third order optical nonlmearity, varying upon central metal atom substitution and other factors. other interesting properties of this molecule and many of its derivative products are their versatility, architectural flexibility and high environmental stability, which are very important requirements to implement photo - electronic applications

    因其骨架結構特徵和可通過選擇中心離子、軸向配體和在酞菁環上引入功能性取代基等方法進行分子篩選與組裝得到具有特殊的物理化學性質和光、電、催化等功能的材料，而引起化學家和材料學家的濃厚興趣。
  18. Crystal zirconium phosphate - phosphonate has the stability and regulity of interlayer floor of inorganic a - zirconium phosphate, they still have the designability of the organic group in the interlayer and adjustment of the ratio of organic and inorganic phosphorus acid, they are a kind of potential smart material, we can process molecule design and optimize filtration according to the requirement of goal reaction, we can prepare special ion - exchange, catalyst, catalyst supporters nonlinear optics compound, solid state proton conductivity and the intercalation complex precusor by introducing different active organic group or active center into the choice and stable framework of inorganic a - zirconium phosphate

    有機?無機晶態混合磷酸鋯具有無機磷酸鋯的層板穩定性和規整性，同時具有層間有機基團的可設計性和有機無機磷酸混合配比的可調節性的特點，是一類很有潛力的靈巧材料，並且可以根據目標反應的要求進行分子設計和優化篩選，在無機磷酸鋯優良穩定的骨架上引入不同的活性基團或活性中心，可以制備出性能獨特的離子篩、催化劑、催化劑載體、非線性光學物質、固態質子導體和制備插層復合物的前驅物。
  19. Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal

    本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究，並與生化位點標記分析法進行比較，旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法，為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。
  20. This text used different biological enzymes to work on dry milling maize powder, by testing the viscosity shown on rva, selected biological enzymes amounts and variety based on viscosity curve, and analyzed their mechanism simply

    摘要採用蛋白酶、脫支酶、乳酸菌胞外酶處理玉米麵粉，用rva測其糊化曲線，篩選出對糊化性質有所改善的生物酶及其用量，並分析其改良機理。
分享友人
分享到 Line

分享到臉書