粒子列表 的英文怎麼說

中文拼音 [lìzilièbiǎo]
粒子列表 英文
list of particles
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 粒子 : grain; granule
  1. Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome

    為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  4. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  5. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  6. The silica coated nanometer particles after containning in n2 for 2h within 600 c is the crystallization form. thus it can sufficiently work the nano effect. the surface of the composite particles has no crystal water and no absorbing water. the anti - oxidation ability increase after coating

    鐵納米復合粒子為結晶態，納米粒子在6000c ，氮氣保護下，納米粒子不重新結晶，殼層二氧化硅包裹在納米鐵表面阻斷了納米粒子之間的重新原子排列。
  7. Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions

    細胞色素b基因序列變異分析表明： 1 ）雁形目鳥類細胞色素b基因全序列長度一致，無插入和缺失：對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列，無無義突變，全序列內部無終止密碼子； 2 ）序列比對后1143加，含416個核著酸變異位點， 306個簡約信息位點，其中處於密碼子第三位的變異最大，第一位和第二位堿基的變異相對較小。
  8. Bonification and centrifu - gation were recommended for the lysis of collected cells and the supernatant and precipitation were collected respectively. as to the plenty of include bodies in the precipitation, denationalization, detergence, purification and dissolution, last regeneration were recommended to acquire great deal of expressed gst fusion proteins

    Coltblzi生產融合蛋白，重組后的dna序列包括一個pgex質粒，依照pgex質粒的融合蛋白的表達是在tao啟動子的控制之下，而枯啟動子可由乳糖的類似物i剛來誘導它表達。
  9. The interface behavior between essence and ethylene - vinyl acetate copolymer pallets was studied so that necessary data were obtained to guide the preparation of fragrant masterbatch. the adsorption type and wettability between essence and the copolymer pallets were analysed by measurements of fourier transform infrared spectrum, surface tension, contact angle and specific surface area. the technical factors affecting absorptivity such as the charge ratio, temperature, pressure and stirring speed were studied by series of adsorption experiments. the results showed that the adsorption of essence on the surface of ethylene - vinyl acetate copolymer pallets is physical in nature. essence couldn ' t moisten the surface of ethylene - vinyl acetate copolymer pallets absolutely, but it could be soaked into the surface of the pallets partly. adsorptivity could be increased by enhancing the temperature, pressure and stirring speed, but the extension of adsorption time had little influence on adsorptivity

    研究了香精與乙烯/醋酸乙烯共聚物粒子之間的界面行為，以便為香型母粒的制備提供必要的理論依據.利用傅立葉變換紅外光譜、表面張力、接觸角及比表面面積等測定手段，分析了香精與載體之間的吸附類型和潤濕作用.並通過一系列吸附實驗，討論了配料比、溫度、壓力、攪拌等工藝條件對吸附量的影響.結果表明，香精在乙烯/醋酸乙烯共聚物粒子表面的吸附為物理吸附；香精無法完全潤濕載體粒子表面，但可以對其形成部分浸潤；提高溫度、壓力、攪拌速度可以增加吸附量，而延長吸附時間對增加吸附量貢獻不大
  10. Abstract : the interface behavior between essence and ethylene - vinyl acetate copolymer pallets was studied so that necessary data were obtained to guide the preparation of fragrant masterbatch. the adsorption type and wettability between essence and the copolymer pallets were analysed by measurements of fourier transform infrared spectrum, surface tension, contact angle and specific surface area. the technical factors affecting absorptivity such as the charge ratio, temperature, pressure and stirring speed were studied by series of adsorption experiments. the results showed that the adsorption of essence on the surface of ethylene - vinyl acetate copolymer pallets is physical in nature. essence couldn ' t moisten the surface of ethylene - vinyl acetate copolymer pallets absolutely, but it could be soaked into the surface of the pallets partly. adsorptivity could be increased by enhancing the temperature, pressure and stirring speed, but the extension of adsorption time had little influence on adsorptivity

    文摘:研究了香精與乙烯/醋酸乙烯共聚物粒子之間的界面行為，以便為香型母粒的制備提供必要的理論依據.利用傅立葉變換紅外光譜、表面張力、接觸角及比表面面積等測定手段，分析了香精與載體之間的吸附類型和潤濕作用.並通過一系列吸附實驗，討論了配料比、溫度、壓力、攪拌等工藝條件對吸附量的影響.結果表明，香精在乙烯/醋酸乙烯共聚物粒子表面的吸附為物理吸附；香精無法完全潤濕載體粒子表面，但可以對其形成部分浸潤；提高溫度、壓力、攪拌速度可以增加吸附量，而延長吸附時間對增加吸附量貢獻不大
  11. This product fits for recycling resin bonded sand and used sand and it is made of two groups rotors with high rotating speed and scraper with low rotating speed. while working, the rotors with high rotating speed and scraper with low rotating speed will cooperate with each other to strike, cut, extrude and rub the sand to break and make the inert film to fall and to make the sand be recycled after separation by wind

    該系列產品主要用於樹脂砂舊砂的再生，它的工作結構是由兩組高速旋轉的轉子和低速轉動的刮板組成，工作時，高速旋轉的轉子與低速轉動的刮板配合，對砂子施以沖擊剪切積壓和搓擦，使砂粒表面的惰性膜破碎脫落經過風選分離，達到再生目的。
  12. In the study, several recombinant expression vectors were constructed in vitro by using the technique of gene engineering, making pheromone 3 be expressed under the control of different promoters and signal pcptides

    本研究利用基因工程的方法，體外構建重組表達質粒，探索用不同的啟動子和信號肽序列表達八肋游仆蟲信息素3蛋白。
  13. Recently, anodic aluminum oxide ( aao ) membranes with a regular porous structure have been prepared by electrochemical methods and have widely been used to produce various nanostructured materials within highly ordered channels in the aao membrane in which the diameters and lengths of the inserted nanowire can be well controlled. in this dissertation, after reviewing the latest development in research of the novel system of nano - array composite by using template method, reports my research work of fabricating metal / aao nano - array composite and their novel optical properties in the past few years. different metal / aao composites ranging in volume fraction of the metal showed unusual size - dependent optical properties, some important results obtained are as following : 1

    接著論文全面總結了作者近幾年以金屬aao納米有序陣列復合結構光學特性為選題的研究工作：進一步優化了制備金屬aao納米有序陣列復合結構的工藝參數，較系統地考察了分別由幾種不同金屬（ co 、 ni 、 ag 、 cu ）植入aao模板后，合成的納米有序陣列復合結構樣品的光反射、透射和吸收等一般光學特性，還專門研究了各系列結構樣品吸收邊的頻移、金屬納米粒子表面等離子激元共振吸收特性，以及特殊的半導體光學特性等。
  14. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  15. Self - assembly of nano - structured silver particle and its surface - enhanced raman spectroscopic application

    銀納米粒子陣列的自組裝及其表面增強拉曼光譜應用
  16. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  17. These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5

    方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ e
  18. Expression of human telomere repeat binding factor1 in acute leukemia cells and its correlation with telomerase activities

    人端粒重復序列結合因子在急性白血病細胞中的表達及與端粒酶活性的關系
  19. Lee p c, meisel d p. adsorption and surface - enhanced raman of dyes on silver and gold sols [ j ]. j. phys. chem., 1982, 86 : 3391

    鄭軍偉，李曉偉，周躍國，顧仁敖.銀納米粒子陳列的自組裝及其表面增強拉曼光譜應用[ j ] .光譜學與光譜分析， 2000 ， 6 : 814
  20. The " water pool " in reverse micelle is formed by surfactant spontaneously arraying in non - aqueous solvent. this provides an excellent micro - reactor for the preparation of nanoparticles

    表面活性劑會在非水溶劑中自發排列形成納米尺寸的「水池」 ，這為納米粒子的制備提供了優良的微反應空間。
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