粒子鑒別 的英文怎麼說
中文拼音 [lìzijiànbié]
粒子鑒別
英文
particle identification-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The scintillation csi was also calibrated with protons of 15, 20, 23 mev. the average energy calibration is 1. 047 mev per channel. energy calibration for heavy ions was completed with multiplication factor 1 / 12. 75
根據能量刻度實驗的結果，我們對四種帶電粒子進行了測試，方法是首先計算粒子的總能量，確定該粒子所屬的能區並計算e ，根據e一e方法來鑒別帶電粒子的種類。The irradiation experimental results show that the resolution of thin detector e1 is higher than that of other thick detectors for ex - particles and protons detection. energy calibration for a - particles was completed with multiplication factor 1 / 3. energy calibrations of a - particles in detector e1 and e1, are 0. 107 mev and 0. 123 mev per channel, respectively
質子能量刻度採用1檔，每道能量h約在0 . 0167mev道，但是在e _ 1探測器中的能量刻度隨著能量的增加略有所下降，這是由於高能質子伴隨有較多的核反應道對粒子探測產生了干擾，從而增加了在e _ 1探測器中的能量沉積道數，使得每道能量略有所下降，另一個因素可能是探測器厚度的不均勻性的影響，但這並不影響對粒子的鑒別。In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。At the surface of the pollen grains there are three types of aperturates, including three porates type, three colpates type and three porate - colpates type ; and four types of sculpture, namely, reticulate, striate, striate - reticulate and verrucate. the upper epidermal cells of the leaves of 12 species plants are detected with hpias - 1000 image analytic system through the treatment of binarization, and the experimental methods are proved stable. the results indicated that the waviness of the anticlinal walls ( sfc ) and the ratio of the feret ' s diameter ( slf ) of the epidermal cells of the middle lamina in the third node of leaves starting from the lowerest part of the stem have a relative constant range and could distinguish from each other. oieanolic acid, l, 5, 8 - trihydroxy - 3 - methoxyxanthone and swertiamarin are separately detected on the tlc, and the different chromatogram of various plants can be considered as characters of identification. the contents of oleanolic acid in 12 species of plants are determinated by hplc, but among the different plants and botanical organs their contents are different, and the highest content in flowers
本文對川鄂產獐牙菜屬藥用植物資源進行了野外調查、標本採集和鑒定，對12種乾燥藥材的性狀進行了描述，提供了可以鑒別的特徵。通過掃描電子顯微鏡觀察的12種本屬藥用植物的花粉粒均為單粒花粉，萌發孔有3孔型， 3溝型和3孔溝型三種類型；表面紋飾包括網狀紋，條狀?網狀紋，條狀紋和瘤狀紋四種類型。用hpias ? 1000高清晰度彩色病理圖文分析系統對葉片上表皮細胞作圖像分析，並進行方法學研究，結果表明，同種植物莖上第3節葉片中段主脈和第1側脈之間葉上表皮細胞垂周壁彎曲程度sfc值和細胞縱、橫向直徑的比值slf值，種間有顯著差異，每種都有相對恆定范圍值。Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。Methods tlc was used to identify the 6 kinds of crud drugs ( lycium barbarum, chrysanthemum morifolium, comas officinalis, paeonia suffruticosa, alisma orientalis, and rehmannia glutinosa ), and all the established methods were used to examine the stability of three batches of the granules at mom temperature and acceleratin conditions
方法以薄層層析法鑒別杞菊地黃顆粒中枸杞子、菊花、山茱萸、牡丹皮、澤瀉、熟地黃等6味藥材；對該制劑3批樣品及加速試驗樣品進行考察。The identification of particles is provided in two ways. on one hand a charged particle detector is attached to phos to discriminate charged particles against neutral particles
通常有兩種方法來鑒別光子和其他粒子：之一，在phos前放置一帶電料子反符合探測器cpv （ chargedparticlevetodetector ） 。Preliminary results indicate that the standard and low electromagnetic interaction packages in geant4 give almost identical simulation on emc, the detector performance, such as energy / position resolution, satisfies requirements of physics design. different behaviors of hadrons and electrons in emc will be helpful for particle discrimination, such as e / discrimination. the complete and perfect offline simulation software will play an important role in physics study at bes
初步結果顯示:標準和低能兩種電磁相互作用模擬軟體包在bes能區給出了相同的模擬結果;探測器的運行指標，如能量解析度和位置解析度，滿足物理設計指標;強子和電子在emc中的不同行為將會給進一步的粒子鑒別（如e /鑒別）提供幫助;完整而完善的離線模擬軟體將在今後bes上的物理研究發揮重要作用。These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5
方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ eIn order to meet the requirement of nano - hexaferrites, hydrothermal method and sol - gel self - propagating high - temperature synthesis method were applied. by studying the effects of different precursors, ph value, temperature and time, the optimum conditions were determined, the structure of crystals was studied by x - diffract aat the same time the morphologic and the diameter of crystals were studied by tem
使用x一射線衍射鑒別晶體結構，通過透射電子顯微鏡觀察粒子的形貌與大小， x一射線衍射鑒別晶體是鋇鐵氧體，透射電子顯微鏡觀察粒子的形態與大小， ，發現粒子分散性較好，結晶性良好，形貌為六角型，通過統計計算粒子粒徑，粒子大小處于納米級，平均粒徑為70nm 。分享友人