純化層 的英文怎麼說
中文拼音 [chúnhuàcéng]
純化層
英文
passivation layer-
After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum
使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。Two kinds of flavonoids in water extracts, water - extraction and alcohol precipitation, alcohol extracts were found by paper chromatography. they were separated and purified by thin - layer chromatography, one of which was primarily identified by uv as 4 ", 5 - dihydroxy - flavonol etherification or 4 ", 5, 8 - trihydroxy - flavonol etherification
水提物、水提醇沉物、醇提物等3種提取物紙層析結果顯示有兩種黃酮類化合物，經薄層層析分離純化，並進行紫外光譜分析，初步測定其中一組分為4 ， 5 -二羥基黃酮醇醚或4 ， 5 ， 8 -三羥基黃酮醇醚。Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column
使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。According to the polarity, the residue was isolated with petroleum ether, chloroform, ethyl acetate, and n - buoh, respectively. the n - buoh fraction, confirmed as neuroactive component, was subjected to sephadex lh - 20 column chromatography to provide an extract fraction, as a buff powder, which could induce neurite outgrowth in rat pheochromocytoma pc12 cells in a dose - dependent manner up to 50 mg / l
將菟絲子乾粉用75乙醇浸泡后，減壓蒸干后得到褐色漿狀物，經石油醚、氯仿、乙酸乙酯、正丁醇萃取，經柱層析后，再用葡聚糖凝膠對有效成份進一步純化，獲得了菟絲子中能誘導pc12細胞分化的活性組分。Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst
Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。The purification and identity of human trophoblast cell
人絨毛膜滋養層細胞的分離純化及鑒定Furthermore, the growth of escherichia coli tg1 and staphyloccus aureur atcc25923 were not under the influence of apss. the switching pulse induced a perceptible sustained transmembrane current on the planar lipid bilayer after unpurified aps added
機理研究表明未經純化的aps作用於脂質平面膜，可以形成跨膜電流，提示活性成分可能通過改變脂質雙層膜通透性抗菌。The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column. the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls, and as such could be considered a ps ii core complex
對于有dcip光還原活性的20和30層帶的復合物，進一步deae離子交換層析純化。 150mmnacl洗脫純化后的樣品經過熒光激發光譜測定發現，已經去除了葉綠素c和墨角藻黃素，並且仍然具有dcip的光活性，分析是ps核心復合物。The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75
從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation
將表達產物破包涵體后經glutathinonesepharose4b柱親和層析分離， sds page顯示純化的蛋白質為一分子量60kd的單一蛋白帶。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1
因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。Mm ). mg2 +, mn2 +, zn2 +, fe2 +, fe3 +, cu2 + have enabled effect on enzyme activation and edta produce a strong inhibitory effect on the enzyme. embranch amino acid have no effect on the enzyme
在離子交換柱層析中，採用不同的ph值及不同類型的緩沖液對純化條件進行優化，最終選擇了ph6 . 0mes緩沖液，並得到了酶蛋白洗脫點為0 . 24 0 . 32mol lnacl 。In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization
本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab
其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected. finally we obtained some light yellow green ointment liquid f i
採用硅膠作干層析柱填充物，用氯仿作展開劑和洗脫液，反復硅膠層析，對柄海鞘鞘囊氯仿浸提物進行分離、提取與純化，最後得到一淺黃綠色的油狀液體fraction （以下均稱f ） 。Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel
融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。分享友人