索菌素 的英文怎麼說
中文拼音 [suǒjūnsù]
索菌素
英文
funiclarin-
Determination of residual ethyl acetate in tiamulin fumarate
延胡索酸泰妙菌素中乙酸乙酯殘留量的測定Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。In order to understand the epidemiological behaviors of this pathogen, we studied strains e. coli 0157 isolated from patients and dung beetles in 1999 and 2000 in xuzhou city, jiangsu province, by methods of pcr, antimicrobial susceptibility, biochemical reaction and pfge ( pulse - field gel electrophoresis ) of chromosomal dna digested by restriction enzyme xbal
為了探索我國大腸桿菌o157 ： h7感染的流行病學特點，我們使用了聚合酶鏈反應、生化反應、抗生素敏感實驗、脈沖電場凝膠電泳等分子生物學方法，對我國1999 - 2000年徐州地區分離到的部分大腸桿菌o157 ： h7進行了分析。To date there is no specific database for toxin and anti - nutrient proteins. in order to establish such a database, we have collected data from some nucleotide and protein database available at present. totally, 1033 toxin proteins, including 172 from plants, 251 from animals, 577 from bacteria and 42 from " other organisms, as well as 1013 lectins and 391 proteinase inhibitors are collected
本文通過對主要基因或蛋白數據庫進行檢索，收集散落於不同基因或蛋白數據庫中的毒蛋白氨基酸序列數據1033個，其中植物毒蛋白172個，動物毒蛋白251個，細菌毒蛋白557個，其它生物如真菌、藻類等的毒蛋白42個；抗營養因子蛋白數據1404個，其中凝集素1013個，蛋白酶抑制劑391個。The physiological character of cellulase and the best culture ph, temperature and stability were quested for. then according to this base, this thesis analyzed the influence of some parameters, including energy and dose for the purpose of finding the best ion mutant parameters, and studied the mechanism of implanted ion on this strain
選擇了纖維分解細菌酶活測定方法以及dns法測定還原糖的最適條件，對纖維素分解細菌的生長特性及纖維素酶的最適溫度、 ph值、溫度和ph值的穩定性進行了探索。分享友人