細小病毒抗體 的英文怎麼說
中文拼音 [xìxiǎobìngdúkàngtǐ]
細小病毒抗體
英文
fpv- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 小 : Ⅰ形容詞1 (體積、面積、數量、強度等不大) small; little; petty; minor 2 (年紀小的; 年幼的) youn...
- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 細小 : very small; tiny; trivial; micr-; micro-; -ling
- 病毒 : [醫學] virus; inframicrobe (濾過性)
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Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody
該法以硝酸纖維素膜為固相載體,包被膜載抗原製成的診斷膜片具有良好的特異性:不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應;膜片具有良好的靈敏性,陽性血清作2 ~ ( - 11 )稀釋亦能檢出;膜片具有良好的穩定性,在4至少能保存7個月,其靈敏性不變。No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞, 72小時后收集培養細胞和上清;培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物,粗略估計表達量約6 7 g 2 10 ~ 6個細胞(相當于egf標準品) ;重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲,每隔24h收集蠶血淋巴,經elisa檢測發現第4天表達量最高,根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ; elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。Vp2 is one of viral structural proteins on the surface of provirus. its neutralizing antigenicity has been proved. in this study
Vp2是鵝細小病毒結構蛋白之一,是gpv的重要保護性抗原,在體內誘導的抗體具有中和作用。This paper describes the development of a sensitive, serogroup - specific elisa for detecting antibodies to mouse parvovirus and distinguishing them from antibodies to minute virus of mice
本篇描述一個偵測小鼠小病毒抗原具敏感性且血清群特異之elisa ,並且可以與其他微細小鼠病毒之抗體區隔。分享友人