細病毒組 的英文怎麼說
中文拼音 [xìbìngdúzǔ]
細病毒組
英文
tenuivirus- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 病毒 : [醫學] virus; inframicrobe (濾過性)
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Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。It refers to the release of the viral genome from its protective capsid to enable the nucleic acid to be transported within the cell and transcribed to form new progeny virions
它指的是將病毒基因組從它的保護性衣殼中釋放出來,使核酸能在細胞內轉運並能轉錄以形成新的子代病毒。Table 1. detection of hcmv in control and chd group
表1 .冠心病組和對照組血巨細胞病毒檢測結果Nayak et al. (1965) studied the immunocytologic and histopatholgic development of experimental swine influenza virus infection in pigs.
Nayak等(1965)研究了豬的實驗性流感病毒感染的免疫細胞學和病理組織學發展。Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法,並試圖在預防上提供有效的免疫制劑,本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化;並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。Results in the 260 slices of liver biopsy, there were 20 ones of acute viral hepatitis, 78 ones of chronic hepatitis, 79 ones of hepatocirrhosis, 28 ones of primary liver cell cancer, 27 ones of liver karyon heterogeneity, 20 ones of liver metastatic adenocarcinoma, 12 ones of fatty liver, five ones of alcohol liver, three ones of liver cyst, two ones of congenital bile duct atresia, six ones of illegible structure, 20 ones without liver cell or with few scattered liver cell
結果260例肝穿活檢組織病例中,急性(病毒性)肝炎20例;慢性肝炎78例;肝硬化39例;原發性肝細胞癌28例;肝細胞核異質27例;肝轉移性腺癌20例;脂肪肝12例;乙醇肝5例;肝囊腫3例;先天性膽管閉鎖2例;穿刺組織結構模糊6例;未穿到或僅穿到少量肝細胞20例。The tlvl offers a wide range of diagnostic services including necropsy, bacteriology, biochemistry, cytology, haematology, histopathology, parasitology, serology and virology. serological and virological testing for avian influenza h5 surveillance and testing for chemical residues in livestock is also conducted
其中包括解剖、細菌化驗、生物化學化驗、細胞化驗、血液化驗、組織病理化驗、寄生蟲化驗、血清化驗及病毒化驗,並提供監察禽流感的血清及病毒化驗與及禽畜The biological functions testified include : enhance iron ion assimilation of epithelial cell of intestine and equilibrate body iron concentration ; broad spectrum of antiviral activity, antibacterial activity and antifungal activity ; modulate marrow cell production and growth ; help to mature and regulate a number of immune cells throughout the body, thus boost body immune ability ; prevents " free iron " from forming free - radicals ; supress tumour growth and prevent tumour formation in animal models
乳鐵蛋白是一種糖蛋白,為轉鐵蛋白家族的一員,在人和哺乳動物的許多器官與組織中廣泛分佈。乳鐵蛋白具有多種生物學功能,這些功能包括:促進人體腸道對鐵的吸收及調節體內鐵的平衡;廣譜抗菌(細菌和真菌) 、抗病毒感染作用等。Hi our study, dendritic cells ( dcs ) were derived from the cultivation of peripheral blood monocytes in vitro successfully. then to observe whether dcs transfected with carcinoembryonic antigen ( cea ) - vaccinia recombinant virus ( rv - cea ) can induces cytotoxic t lymphocyte - mediated cea - specific immunity in vitro
體外成功地完成了cd14 +單核細胞來源的樹突狀細胞( dc )的培養,並進一步研究人癌胚抗原重組痘苗病毒( rv - cea )轉染dc后體外誘導的cea特異性細胞免疫。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。Baculovirus / insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium
摘要:桿狀病毒/昆蟲細胞系統已經被廣泛的應用在重組蛋白質的生產上,但傳統的桿狀病毒感染后會造成細胞溶裂,而使得表現出的重組蛋白質流失到培養基中。In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not
合成該套式引物,選擇大熊貓原代病料和病毒各代細胞培養物,經套式( nested ) rt一pcr擴增,可得到一與設計值5巧bp相符的dna片段,經bst一xl ( 590 , 1110 )酶切鑒定,證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段,純化,送生物公司測序。Furtherly expression phase analysis showed that orf2 appeared in different form during infection. to reveal the function of haorf2, vha - orf2 - gfp recombinant virus which tagged with egfp - orf2 fusion protein was generated
構建了ha2與綠色熒光蛋白融合表達的重組病毒vha - orf2 - gfp ,對該重組病毒感染細胞后,融合篡碩士學位論文mast卜r 』 s 』 r拼ifsThis paper expounds the progresses of the researches on the life science and relevant subjects, which include the research of cultivating the arterial vessel with fine cell tissue, the research on the genetically modified animal, the research on the pest control with bio - virus pesticide, the research on the death mechanism of protein and the diseases of immune system ( including cancer ), the research on anti - senile protein and the research on compound - type aids vaccine
闡述了生命科學及其相關學科的研究進展,包括用細小細胞組織培製成動脈血管的研究、轉基因動物的研究、生物病毒農藥防治蟲害的研究、蛋白質死亡機理及免疫系統疾病(包括癌癥)的研究、抗衰老蛋白的研究以及復合型艾滋病疫苗的研究。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2
實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒,並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白,重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長, k 。Standard practice for preservation by freezing, freeze - drying, and low temperature maintenance of bacteria, fungi, protista, viruses, genetic elements, and animal and plant tissues
用冷凍冷凍乾燥和低溫養護法保存細菌真菌原生生物病毒遺傳要素以及動物和植物組織的標準實施規程No recipient of group a ( without any treatment, n = 5 ) or group b ( treated with adgfp, n = 4 ) died within three weeks after transplantation and severe acute rejection ( massive periportal infiltrate, endothelilitis, damage to biliary epithelium and severe tissue destruction ) was confirmed by pathological examination of the graft. in contrast, all recipients of group c ( treated with adhuctla4 - ig, n = 5 ) achieved lohg - term liver allograft survival ( > l50d )
35 ,屍0刀5八而灌注攜帶融合基因a個ig的重組腺病毒組組, fi ? 5 )受體鼠均能獲得長期生存( 150天) ; a組與c組間、 b組與c組間,生存期均有顯著性差異』 ( p 0刀1人a組與b組,在術后8天行移植肝活檢,病理學檢查證實:移植肝均發生嚴重的細胞性排斥。分享友人