細胞光度測定 的英文怎麼說

中文拼音 [xìbāoguāngdùcèdìng]
細胞光度測定 英文
cytophotometry
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 光 : Ⅰ名詞1 （照耀在物體上、使人能看見物體的一種物質） light; ray 2 （景物） scenery 3 （光彩; 榮譽） ...
  • 度 : 度動詞[書面語] （推測; 估計） surmise; estimate
  • 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  • 測定 : determine; determination; setting-out; admeasurement; assignment; assay; finding
  1. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  2. The purposes of the present study were to investigate ( 1 ) the hemodynamic effects of agmatine in anaesthetized dahl salt - sensitive ( ds ) hypertensive and dahl salt - resistant ( dr ) rats ; ( 2 ) the effect of agmatine on vascular tension in the isolated aortic artery of rats and the underlying receptor mechanism ; ( 3 ) the effects of local injection of agmatine on femoral, renal, and mesenteric vascular beds by constant flow perfusion method ; ( 4 ) the effect of agmatine on l - type calcium current ( / ca - t ) in rat ventricular myocytes with whole - cell configuration of the patch - clamp technique ; ( 5 ) the effects of agmatine on free intracellular calcium concentration ( ca2 + d of isolated rat ventricular myocytes

    （ 3 ）採用後肢、腎臟和腸系膜動脈在體恆流灌注法，觀察向灌流環路中直接注射胍丁胺的血管效應。 （ 4 ）應用全細胞膜片箝技術，觀察胍丁胺對大鼠心室肌細胞l -型鈣通道電流（ i _ （ ca - l ） ）的影響。 （ 5 ）用fluo3 - am負載分離的大鼠心室肌細胞后，由激光共聚焦法測定單個心室肌細胞[ ca ~ （ 2 + ） ] _ i的熒光強度，觀察胍丁胺對分離大鼠心室肌細胞內游離鈣濃度（ [ ca ~ （ 2 + ） ] _ i ）的影響。
  3. The system measures photosynthesis rate by using infrared co2 gas analyze method. it has two work modes : open route and close route. it can measure the leaf photosynthesis rate, transpiration rate, stomata conductance and co2 thickness in cell clearance etc parameters about plant physiology

    本文研究設計了測定光合、蒸騰速率的主從式虛擬儀器系統，系統採用紅外線分析法測定光合速率，設置有開路和閉路兩種測定方式，可以測量植物葉片的光合速率、蒸騰速率、氣孔導度和細胞間隙co _ 2濃度等與植物光合作用相關的參數。
  4. 2. mouse lung vessel endothelial cell was used for examining ha, hb and hpd ' s ic50 concentration under saturated light dose and ic50 light dose in saturated concentration irradiated by copper vapor laser of 510. 6nm and 578. 2nm separately

    應用體外培養的小鼠肺血管內皮細胞系（ 1h11 ）測定在飽和光劑量的條件下，實驗光敏劑對血管內皮細胞的殺傷作用，得出其半數殺傷濃度。
  5. In the second part, the influences of la on micronucleus rate were observed by using the rat marrow cell micronucleus test. and the cleavage action of la on genome dna were studied too. the results manifest that a certain concentration of la can increase micronucleus rate obviously and induce the cleavage action and structural change of genome dna

    （二）採用小鼠骨髓細胞微核檢測技術研究了稀土元素鑭對微核率的影響，同時採用體外培育技術和紫外分光光度法研究了鑭對基因組dna的斷裂作用，結果表明一定濃度的鑭能引起微核率顯著升高，並可導致基因組dna的斷裂以及結構的改變。
  6. 2 mtt assay was used to examine od value of terasaki wells. od value was measured once a day for 8 days and mapped growing curve of cells to determine the activation of proliferation in primary cells

    2採用mtt比色法測定細胞的光密度值（ 0d值） ，每24h測定1次，從細胞接種到神經球形成連續測定sd ，繪制細胞增殖活性曲線。
  7. We cultured photosynthesis bacteria ( psb ), using trfe mixed substances as an additive of culture medium. through the analysis of residue of the mixed substances in the culture media and od660nm of cell concentration, it revealed that cell concentration became higher, and residue content of the mixed substances was lower in a week

    對細胞濃度（ od _ （ 660nm ）值）和氣態混合物的殘留成分測定，結果表明添加了氣態混合物的光合細菌濃度明顯高於未添加的，而且培養基中的氣態混合物經培養一周后明顯減少。
  8. To investigate the relationship between optical density ( od ) and the biomass of phaeocystis globosa. p. globosa was used under laboratory conditions to study the relationship between the cell density and its related od

    在實驗室中測定棕囊藻細胞密度及光密度（ od值）並進行直線回歸分析，同時採用光密度值來估算棕囊藻的生物量。
  9. In our research, marked autologous fluorescent blood red cell is immitted into sd - rat body and the whole process is shown and recorded by microscope image system. after these processes, we can replay the recorded tape and sample images with video image card. then, we use sequence image processing to analysis the image of dark ground microscope

    利用做過熒光標記的自身紅細胞注入sd大鼠體內，通過顯微圖象系統將整個過程以視頻信號的形式存貯，然後利用基於視頻圖象的採集卡，將流速變化過程回放采樣，得到暗視場下的熒光圖象，利用圖象分析和處理的方法，測定血流速度。
  10. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  11. And the mechanism of action of medicine to cell can be researched form biomedicine ; ( 3 ) the ca2 + concentration in b16 cell was measured with ratio fluorescence imagemaster. the preparation of cell and the carry method of fluorescence indicator were confirmed ; the acquisition of cell image and the ratio analysis method by software - imagemaster were established ; the acquisition of cell fluorescence image and the ratio analysis method by software - felix were established

    （ 3 ）通過用fura - 2 / am對b16細胞內ca ~ （ 2 + ）濃度的測量，確定了b16細胞的制備及熒光染料的載入方法；建立了用隨機軟體imagemaster獲取被測細胞的圖象及進行比率分析的方法，用隨機軟體felix獲取被測細胞的熒光圖譜及進行比率分析的方法。
  12. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
  13. Multiple kinase assay was performed to examine pkc ^ mapk. tpk activity in the transfected cells. meantime, pegfp - sh2a vector was also constructed and the cells transfected with it were examined by fluorescent microscopy. the expression of sh2a gene was examined under different concentration and time of bfgf as a stimulating factor

    1 sh ，利用脂質體轉染肝癌bel7402細胞人os7細胞，檢測pkc 、 mapk 、 tpk活性的改變；流式細胞儀檢測細胞增殖；另構建pegfp sh ，轉染細胞，熒光顯微鏡觀察熒光定位； bfgf作為刺激因素處理細胞，根據不同濃度、時間檢測sh基因表達情況。
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