細胞冷凍 的英文怎麼說

中文拼音 [xìbāolěngdòng]
細胞冷凍 英文
cell freezing
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 冷 : Ⅰ形容詞1 （溫度低; 感覺溫度低） cold:冷水 coldwater; 你冷不冷? do you feel cold?; are you feeling...
  • 凍 : Ⅰ動詞1 （液體或含水分的東西遇冷凝固） freeze 2 （受冷或感到冷） feel very cold; freeze; be frostb...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  3. Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52

    以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。
  4. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  5. All samples of the tumor tissues were observed by electron microscope. the t lymphocyte subsets and b lymphocytes were detected and counted with monoclonal antibody technique before and after the cryotherapy. results : the cured percentage of internal hemorrhoid, mixed hemorrhoid and chronic fissura ani reached 83. 5 %, 66. 8 % and 62. 4 % respectively. the clearance rate of the verruca acuminata was 100 %

    方法：取自西京醫院15年間冷凍治療肛腸區良性病變7018例，惡性病變636例，冷凍前後經電鏡觀察癌細胞變化及應用單克隆技術檢測患者t細胞亞群、 b細胞水平。
  6. Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency

    多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。
  7. F ig. 1 samp le p repared by h igh p ressure freezing and freeze subst itut ion ( s. sheath )

    圖1經高壓冷凍固定和冷凍置換制備的發菜營養細胞（ s .膠質鞘） 。
  8. Beneficial effect of intracellular trehalose on viability of freeze - dried red blood cells

    胞內海藻糖對紅細胞冷凍乾燥保存效果的影響
  9. Effect of vitrification freezing on the chromosomes and spindles of porcine oocytes maturation in vitro

    玻璃化冷凍對豬體外成熟卵母細胞染色體與紡錘體的影響
  10. The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body

    小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑，其制備、傳代及冷凍保存均有不同的研究報道，飼養層的質量受許多因素的影響，如動物的品種、培養液、所傳代數及實驗條件等，關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少，故本實驗以昆明小白鼠為實驗動物，研究其胚體培養過程中細胞的形態學、組織學等方面的變化，以期為本實驗室建立飼養層細胞庫提供理論依據，同時探討心肌細胞和成纖維細胞層共培養的可行性，以期為心臟生物學特性的體外研究奠定基礎。
  11. Effects of different freezing methods on the proliferative competence of follicle cells in rabbit ovarian tissue

    不同冷凍方案對家兔卵巢組織卵泡細胞增殖活性的影響
  12. ( 3 ) murine embryonic body fibroblast cells could be refrigerated well by method of embryonic freezing. the cells motility thawed was 0. 86

    （ 3 ）胚胎冷凍法可以使成纖維細胞取得較好的冷凍保存效果，解凍活率最高可達0 . 86 。
  13. Methods : we took the method of braking cells machinerily, dialysis in low temperature, freeze - drying and son on to enhance its efficacy of oral transfer ring - factor

    方法：通過機械法破碎細胞、低溫透析、冷凍乾燥等工藝手段來提高口服轉移因子的藥效。
  14. Shows how fibroblasts from human dermis are isolated by using enzymes and then cultured in nutrient medium

    示?如何使用酵素分離人類皮膚的纖維組織母細胞在營養培養基培養，如何計算數目及液體氮冷凍儲存法。
  15. The vitrified cryopreservation of animal tissues and cells is a new ultra - low temperature freezing method, several animal tissues and human embryos have been successfully cryopreserved, this method will be of great value to application and research

    摘要動物組織細胞的玻璃化凍存是一項新的深低溫冷凍保存方法，已成功地凍存了多種動物細胞，具有較高的實際應用和研究價值。
  16. Cryopreservation of peripherial stem cells at - 80 and its clinical application

    直接冷凍保存造血幹細胞的技術及其臨床應用
  17. Because the frozen sections revealed no residual tumor cell after tumor excision, canaliculostomy and semicircular flap was performed simultaneously

    病人在接受腫瘤切除送冷凍切片證實沒有殘存癌細胞后，接受淚小管造口手術及眼瞼半圓形皮瓣重建。
  18. This clinical report introduces a novel material ? freeze dried acellular dermal matrix allograft ? to serve as a grafting material for the purpose of increasing attached keratinized gingival, and makes some advices to help in raising the predictability of the result

    本臨床報告介紹一種新的材料冷凍乾燥無細胞皮膚基質同類異體移植體作為增加角化附連牙齦手術的植體材料，同時也提出一些建議，以提高手術的可預測性。
  19. Experimental study on effect of pretreatment with glycerol upon freeze - drying preservation quality of red blood cells

    甘油預處理對紅細胞冷凍乾燥保存作用的實驗研究
  20. Players are freezing the cells taken from the umbilical cord blood of their babies as a possible future cure for cartilage and ligament problems

    球員將孩子臍帶血中的幹細胞冷凍，以便今後可以用於治愈軟骨和韌帶疾病。
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