細胞分選 的英文怎麼說

中文拼音 [xìbāofēnxuǎn]
細胞分選 英文
cell sorting
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation

    簡要綜述了生物技術在水稻遺傳育種中的應用，主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。
  3. Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen

    試驗二，不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一，進入正式試驗期后，在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠，稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液，並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血，姬姆薩-瑞氏染色，計算t淋巴細胞轉化率，計算免疫器官指數，測定肝臟、脾臟銅含量。
  4. Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library

    我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法，獲得了對酵母細胞即無毒性作用，又沒有自激活作用的「誘餌」 ，通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。
  5. These confirmed the successful transformation of the a78 - 3 and a78 - 4 on selection medium containing basta. microarray on membranes were fabricated from a set of 384 pinus taeda genes ( cdnas ) related to lignin synthesis, adaptation or primary metabolism for examination of gene expression in the sublines. the results showed : ( 1 ) the correlation coefficients between the transgenic sublines a78 - 3 and a78 - 4 and the untransformed control a95 : 88 : 22 were 0. 8028 - 0. 9028, while those of a78 - 5 are 0. 8897 - 0. 9302

    選擇384個與木質素生物合成及植物生理代謝和環境適應性有關的基因或cdna片段構建尼龍晶元膜，並對轉基因細胞亞系a78 - 3 、 a78 - 4和a78 - 5和對照亞系a95 ： 88 ： 22等培養再生植株進行基因表達的微陣列檢測，結果表明： （ 1 ）三個亞系與對照之間的pearson相關系數分別為0 . 8607 、 0 . 7975和0 . 9630 。
  6. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  7. The development of a multicellular organism results from the selective expression of genes and the maturation of proteins is dependent on molecular chaperons

    多細胞生物體發育的實質是基因的選擇表達性，表達蛋白的成熟則有賴于分子伴侶的協助。
  8. Magical fire ( eye doctor ) looks at well - being cream of sticking drawing tradition traditional chinese medicine formula famous and precious in tsinghua, is tied in wedlock modern up - to - date medicine result of scientific research, the various chinese medicinal crop famous and precious being carefully chosen, adopt the modern nano - technology and target to poison a technology to being given to, let various active material, tiny molecule, nutrition factor glutathione etc. guide medicine it is all right for to go ahead, the brute force passes through blood eye parclose, make pesticide effect reach nidus directly location, prompt the nutrition replenishing an eye with the part ( include ciliary muscle, retina, crystalline lens, optic nerve ), active eye part cell, improve eye part immunocompetence and oxidation resistance, boost an eye part organizing an assimilation of the new and excretion of the old, microcirculation improving and restoring an eye part, thereby reach eliminate look at strain, purpose improving and improving sight

    清華神火視康貼汲取傳統中藥名貴配方之精華，結合現代醫藥最新科研成果，精選多種名貴中藥材，採用現代納米技術和靶向給藥技術，讓多種活性物質、微分子、營養因子谷胱甘肽等引藥上行，強力穿透血眼屏障，使藥效直達病灶部位，迅速補充眼部（包括睫狀肌、視網膜、晶狀體、視神經）的營養，激活眼部細胞，提高眼部免疫能力和抗氧化能力，促進眼部組織新陳代謝，改善和恢復眼部微循環，從而達到消除視疲勞，改善和提高視力的目的。
  9. Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1

    我們以小鼠為模型，運用組織化學、免疫熒光組織（細胞）化學、流式細胞儀分選方法（ facs ）以及分子生物學手段，研究了小鼠乳腺的發育規律：小鼠乳腺組織中類乳腺幹細胞：小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定；小鼠類乳腺幹細胞分化的潛能；小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明： 1
  10. Abstract : selective mechanisms exist in organism and biosphere, which select the cells with less dna injuy and reproduces them. there are two models ; mechanisms 1 and mechanisms 2, represented respectively b bacteria and mechanolytes of human beings. cells with mechanisms 1 ( e. g. bacteria, generative cells, cancer cells ) can reproduce infinitely, while somatic cells can not. there are deep - going reasons behind the two categories of selective mechanisms. the canceration of the cells is a degenerating process from mechanisms 2 to mechanisms 1

    摘要生物界存在一類篩選機制，它將dna損傷較小的細胞篩選出來，產生後代.主要有兩類模式機制1和機制2 ，分別以細菌和人的成纖維細胞為代表，那些具有篩選機制1的細胞（如:細菌、生殖細胞、癌細胞）能無限傳代，而體細胞則不能.這兩類篩選機制背後有更深刻的原因，而細胞的癌變正是從機制2退化為機制1的過程。
  11. For the purpose of exploiting the affluent resource of traditional chinese medicines, rat pheochromocytoma pc 12 cells were used as a model to screen the neuroactive components from natural products. most of all, the effect of it on inducing the differentiation of pc 12 cells and / or execute the neuroprotective effect challenged by serum - deprivation and oxidative pressure, and its mechanism were studied in this paper

    本文以利用我國豐富的天然藥物資源為目的，用大鼠嗜鉻神經瘤細胞pc12作為模型，篩選能模擬ngf的功能誘導pc12細胞分化、對神經細胞有一定保護作用的天然藥物，並對其作用機制進行初步探索。
  12. And the differentiation of bmscs were determined to be chondrocyte after g4i8 selection. the bmscs in controlled group were dead about 1 week after g4, 8 selection. conclusions : 1

    對照組bmscs轉染后增殖活性較實驗組低，沒有向軟骨細胞分化的趨勢，經g _ （ 418 ）篩選后1周左右全部死亡。
  13. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  14. During the culture of the porcine ear skin fibroblasts, the successful rate was increased by selecting culture methods, contrasting donor age and improving culture condition. the rate of living cells in the method of combining trypsin cold treatment with trypsin heat treatment was much higher than that in the method of trypsin heat treatment. the results showed that the method of combining trypsin cold treatment with trypsin heat treatment had less adverse effects on cells than the method of trypsin heat treatment

    在培養豬耳皮膚成纖維細胞時從篩選細胞分離方法、比較供體年齡和改善培養條件這三方面著手提高培養的成功率。實驗中發現胰蛋白酶冷熱處理結合法培養的細胞存活率明顯高於胰蛋白酶熱處理法，說明使用胰蛋白酶冷熱處理結合法對細胞的不利影響較少，細胞產量較高。
  15. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  16. Here we report a simple and effective method to induce escs into neurons, and with the help of genechip analysis we can confirm the induced cells as neurons and find some useful or specific genes in neural conversion as well

    本實驗擬摸索出一種簡便有效、無毒性的體外大比例誘導鼠胚胎幹細胞分化為神經細胞的方法，並進一步通過基因晶元分析證實誘導結果，篩選神經細胞分化主導基因。
  17. In addition to the required courses in emergency and critical care medicine, the institute offers a wide range of courses, including instrumental analysis, epidemiology and biostatistics, general immunology, molecular cell biology, advanced emergency medicine, environmental emergency medicine, infectious disease, traumatology, toxicology, bioethics in critical care, seminar, organ transplantation, medical economics, animal models and technology of medical researches, physiological signal processing, pediatric emergency and critical medicine, geriatric emergency and critical medicine, emergency and critical medicine in obstetrics and gynecology, thesis preparation, symposium, etc

    本所目前的課程除了必修的進階急診醫學及進階重癥醫學外，尚有選修的儀器分析臨床流行病學概論及生物統計免疫學概論細胞分子生物學進階急診醫學環境急癥醫學感染學外傷學毒物學重癥生命倫理學器官移植醫療經濟學醫學研究動物模式與技術生理訊號分析兒童急重癥醫學老人急重癥醫學婦產科急重癥醫學論文撰寫專題討論等。
  18. This prescription selects rare chinese traditional medicines that have effects of blood fat reducing and weight reduction, enhancing the secretion of thin factor by brown adipose cells. and it is made with extra care of the essence extracted with japanese scientific extraction technology

    本方精選降脂減肥，提高人體棕色脂肪細胞分泌瘦素作用的名貴中藥，且採用日本高科技萃取工藝萃取精華精製而成。
  19. Experimental condition optimization study on inducing mouse embryonic stem cells differentiation into insulin producing cells by serum - free culture in vitro

    無血清培養法誘導小鼠胚胎幹細胞分化為胰島素分泌細胞的實驗條件優化選擇
  20. The institute provides comprehensive curricula in general chemistry, analytical chemistry, organic chemistry, and biochemistry to medical, dental, nursing, medical technology, and undergraduate life sciences students. the goal of teaching is to help students develop skills that allow them to continue teaching themselves through their careers. individually designed courses are provided for students from different departments and tailored to their career needs in academic and clinical medicine

    研究生所修習課程之規劃是以必修之生化與細胞分子生物學生化技術原理等核心課程提升學生學術基本能力，佐以每年積極開設之包括各式新領域的選修課程，使學生得到更進深的知識，並能擴展學識范疇符合科技發展潮流課程中老師注重學生邏輯思考，力求培養學生獨立發掘與解決問題之能力。
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