細胞均質化 的英文怎麼說
中文拼音 [xìbāojūnzhíhuà]
細胞均質化
英文
cell homogenization- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 均 : Ⅰ形容詞(均勻) equal; even Ⅱ副詞(都; 全) without exception; all
- 質 : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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The distribution of the brine shrimp hgcs varies greatly from the species studied till now. one hour after hatching, neither the dorsal - anterior area nor the other dorsal area remained positive immunoreactivity signal. and 2 hours after hatching, there was no typical hgcs in the body of the brine shrimp and the remained hatching enzymes may participate in digesting the left vitellin in the nauplius
鹵蟲hgc最初出現至孵化前1h時均為全身性分佈,從孵出到孵出后2h ,頭鹵蟲孵化酶的生物化學性質及孵化腺細胞的免疫組織化學研究部的孵化酶顆粒已經減少,而變為非全身性分佈,到孵出后sh ,孵化酶顆粒已基本消失殆盡。Inhibitors of cyp51, such as azalanstat ( rs - 2i607 ) and rs - 2i745, could inhibit the synthesis of ff - mas to decrease the accumulation of ff - mas. inhibitor of a14 - reductase, such as ay9944 - a - 7, could inhibit the metabolism of ff - mas to increase the accumulation of ff - mas ; and some other reagents, such as nystatin, could combine with the downstream intermediate in the cholesterol biosynthesis pathway to accumulate mas. in this study, we investigated the role of mas by using these reagents to change the level of endogenous ff - mas
抑制cyp51酶的抑制劑,如azalanstat ( rs - 21607 )和rs - 21745等,均能抑制mas的合成,降低mas的積累;而抑制14 -還原酶的抑制劑,如ay9944 - a - 7等,能抑制ff - mas向t - mas的轉化而造成ff - mas的積累;還有一些物質,如制黴菌素,能阻止mas向下游的代謝而造成其積累,本文主要通過應用這些物質降低、或增加組織細胞中內源性mas的積累,來研究mas的作用。Berman as per you love dandle tone versus different kinds of sickness and pestilential resistance body nutritional requirements custom - made product , maintain extra alimentation required intensify immune system functional component , match with proportion protein cum fat ingredient give support to immune system combine intensify cell function up
伯曼根據您愛寵增強對各種疾病和瘟疫的抵抗力身體營養需求定做的產品,含有額外營養所需強化免疫系統功能成分,配以均衡蛋白質及脂肪成分支援免疫系統並強化細胞功能。The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone. during the development of pancreas in alligator sinensis embryos, somatostatin ( ss ) immunoreactive ( ir ) cells, 5 - hydroxytryptamine ( 5 - ht ) - ir cells, glucagon ( glu ) - ir cells, epidermal growth factor ( egf ) - ir cells appeared on 18th day. no p53 protein - ir cell, gastrin - ir cell, testosterone - ir cell, chromogranin a - ir cell, vasoactive intestinal polypeptide - ir cell, epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos
本實驗採用免疫組織化學技術,應用13種不同的抗血清,對孵育時間8 ? 55天揚子鱷胚胎消化道及消化腺內分泌細胞的種類進行鑒別、定位和比較,結果如下:揚子鱷胚胎胰腺中,生長抑素、 5 ?羥色胺、胰高血糖素、表皮生長因子、胰多肽免疫反應陽性細胞出現于第8天; p物質免疫陽性細胞出現于第18天; p53 、胃泌素、睪酮、嗜鉻素a 、血管活性腸肽、上皮膜骯原、胰島素免疫陽性細胞在各期揚子鱷胚胎胰腺中均未發現。The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body
小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑,其制備、傳代及冷凍保存均有不同的研究報道,飼養層的質量受許多因素的影響,如動物的品種、培養液、所傳代數及實驗條件等,關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少,故本實驗以昆明小白鼠為實驗動物,研究其胚體培養過程中細胞的形態學、組織學等方面的變化,以期為本實驗室建立飼養層細胞庫提供理論依據,同時探討心肌細胞和成纖維細胞層共培養的可行性,以期為心臟生物學特性的體外研究奠定基礎。Functions : this product contains “ antifungal active protein ” with optimal and strong sterilization effect, which could strengthen the immunity of body fluid, activate the macrophage, strengthen the phagocytosis ability of macrophage, strengthen the body immunity, restrain the growth, pervasion and transfer of abnormal cells, the product has strong sterilization effect and could strengthen the disease resistance ; it is remarkably effective in improving the immunity and improving the infirm constitution of pets ; the amino acid content and composition in the product are moderate and rational, with the characteristics of strong palatability, nutrition balance and immune element abundance, etc
功能:本產品中擁有極佳強烈殺菌作用的「抗菌活性蛋白」 ,能增強體液免疫功能,活化巨噬細胞,增強其吞噬能力,可增強機體免疫力,抑制非正常細胞生長、擴散和轉移,具有強烈的殺毒作用,增強抗病性;對提高寵物免疫力,改善虛弱體質有顯著效果;其中氨基酸含量適中、組成合理,具有適口性強、營養均衡和免疫物質豐富等特點。Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa
將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株( zj111 / pcdan3 - f ) ,並直接轉染vero細胞,分別提取細胞總dna和總rna , dig標記探針均可檢測到陽性雜交信號。It can be concluded that the elevated ability of penaeus immune system is realized via increasing po production instead of increasing of unit enzyme activity of po. since the biggest number of hemocytes, semigranular cells and po production have been obtained after - 1, 3 - glucan and lps stimulation, it implies that the - 1, 3 - glucan and lps would be the most efficient immunostimulant on shrimp, penaeus chinensis, among the immunostimulants used
透射電鏡下的觀察結果表明,中國對蝦血細胞的超微結構在免疫刺激前後也發生了變化,三種血細胞的糙面內質網、核糖體和線粒體的數量均有一定程度的增加,其中以小顆粒細胞和大顆粒細胞的超微結構變化幅度最大。Results the levels of endotoxin, leukocyte, mda and sod were all signifificantly higher than control group, while, the level of glutathione ( gsh ) was much lower in experimental group ( p < 0. 01 )
結果模型組內毒素、肺勻漿脂質過氧化物,以及白細胞計數均明顯增高;而還原谷胱甘肽( gsh )明顯降低,與對照組比差異有顯著性( p < 0 . 05 ) 。Conclusions electron microscopic obser vations show that the alveolar epithelial cells, endothelial cells and basement membrane are all injured, interstitial macrophages hyperpplasia, mast cells infiltration and degranulation, electron - dense deposits at alveolar wall are present
結論電鏡觀察可見特發性肺纖維化時肺泡上皮細胞、血管內皮細胞及基底膜均有損傷,並可見間質巨噬細胞增生,肥大細胞浸潤及脫顆粒,肺泡壁電子緻密物沉積。The results were as follows : ( l ) the pre - incubation of pmr induced the exocytosis of the cortical granules ( cgs ) in the oocytes and reduced the quantity of the microvillus on the surface of oocytes ; ( 2 ) the matrix of cg might migrate to the surface of plasma membrane of oocytes after pmr pre - treatment. ( 3 ) incubation of zona - free hamster eggs with spermatozoa pretreated with pmr a ntiserum was associated with a dose - dependent significant reduction in the number of spermatozoa binding to the oolemma, compared with a contraband with a dose - dependent reduction of the fertilization rate and the penetration index. the results indicate that mr of human sperm plays an important role in sperm - oocyte fusion and oocyte activation
結果顯示: ( 1 )人精子mr能夠誘發去透明帶金黃地鼠卵母細胞中皮質顆粒的胞吐,並引起卵母細胞膜表面微絨毛形態和數量發生改變; ( 2 )皮質顆粒胞吐后,其內容物可結合到卵膜表面; ( 3 )抗mr抗血清預處理精子可抑制精子與卵子的結合與融合,使受精率、穿透指數和結合指數下降,並均有劑量依賴性。以上結果提示,人精子mr在受精過程中起重要作用,其主要作用可能是介導精-卵膜融合併促進卵母細胞的活化。3 in the light and dark, the contents of soluble protein, the activities of peroxidase ( pod ), superoxide dismutase ( sod ) and esterase ( est ) were studied, and they all showed their changes respectively. 4 the alkaloids were accumulated primarily in the stationary phase
4 、培養細胞中次生代謝物質的積累:光照和黑暗條件下生物堿的含量變化不盡相同,但第12d均出現一個峰值,並且從第18d起生物堿的含量呈上升趨勢。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp , r _ 1長36bp ,其它片段均40bp長, f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應,然後用其他單鏈片段作為引物,進行pcr擴增,用dna快速純化回收試劑盒回收所得254bppcr產物,與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞,利用藍白斑遺傳學篩選法篩選陽性克隆,提取其質粒,採用nco和xho雙酶切鑒定,獲得了254bp的片段;用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定,獲得300bp的片段。Icso values of tps and egcg against d6 and wi - 38 are 71. 1 u g / ml, 1786. 7 u g / ml and 58. 6 u g / ml, 2177. 4 u g / ml respectively. lower concentrition of tps and egcg increased the number of wi - 38 and higher concentrition of tps and egcg also can inhibit the growth of wi - 38 cell and is concentration - dependent
Egcg作用d6細胞后採用hoechst33258熒光染料染色並且在熒光顯微鏡下觀察,發現隨egcg作用濃度的增大,細胞出現染色體邊集、 dna斷裂、染色質環化等現象,而對照細胞的細胞核質呈現均一的顏色。Serous cortisol content in each group increased, while cortisol content in group i was higher than that in each other group ( p < 0. 05 ). serous - ep content in each group increased and then decreased gradually, but - ep content in group iv has a significant increased at half an hour following incision ( p < 0. 05 ). conclusions canine immune functions are suppressed by operation with different anesthetic techniques to different degree, but resume at 15th day after operation. there is an interacting and interadjusting mechanism among the cns ( central nerve system ), the endocrine system and the immune system
結果各組在麻醉手術后4h及術后1d白細胞總數、嗜中性分葉狀粒細胞數顯著增加( p 0 . 05 ) ,淋巴細胞數減少;血清球蛋白含量先降低后升高;組rbc ? c _ 3br花環率先降低再升高后降低,其它組均先降低; t淋巴細胞轉化率均呈現不同程度的降低,在術后7d左右恢復正常;血漿皮質醇濃度先升高后降低,但組皮質醇含量在切皮后30min較其它組上升顯著( p 0 . 05 ) ;血漿- ep濃度先升高后降低,但組- ep含量在切皮后30min較其它組上升顯著( p 0 . 05 ) 。It was found that these three different embedded scaffolds could promote adhesion, proliferation, differentiation and matrix secretion of the cells. there was no obvious difference among them, but all of them were more excellent than unembedded one
發現採用型膠原蛋白,鼠尾膠原蛋白和多聚賴氨酸包埋的pdlla ha無紡網均有促進軟骨細胞粘附、增殖、分化和產生基質的作用。Applied constant keeper can enable the microorganism to continuously culture in double periods of “ constant flowing method speed ”. ? it has a process as such : according to the multiplying speed that the bacteria cultures in double growing periods, and the speed that it consumes the nutritious material, fresh culturing liquid is continuously added. ? at the same time, the same amount of well - cultured bacteria liquid is continuously drawn out, so that the next two items are kept relatively constant : the added material and the consuming material ; the amount that the bacteria have multiplied and that has been drawn out
應用恆化器,能使微生物以對數期「恆流法速度」連續培養,即根據菌種在對數生長期繁殖的速度和消耗營養物質的速度,不斷加入新鮮培養液,同時不斷抽出等量培養好的菌液,使加入物與消耗物、細胞繁殖量與細胞物質抽出量,保持相對穩定均恆。In this system the properties of mcs are critical effect to cell culture, in fact, there is still no one perfect mc in all - present commercial products. therefore, it is necessary to develop superior quality mcs
此種模式的細胞培養對微載體的質量要求很高,而當前商品化的微載體均有其不完善之處,因此,理想微載體的開發仍然是微載體研究的方向。From 36hr to 72hr after infection, the fluorescence gathered gradually to the cytoplasmic membrane, and eventually there was no fluorescence remained in nuclear and cytoplasm, and the membrane accumulated high level of fluorescence, and then there was no change taken place from 72hr to 168hr. but from 36hr to 168hr, there was no change took place in the control
感染后36hr ,實驗組細胞中綠色熒光的分佈出現了變化,在細胞核和細胞質中均有綠色熒光分佈,且分佈有一定的規律,在細胞核中核膜處的綠色熒光的強度增強,細胞質中綠色熒光的強度在增加,綠色熒光熒光逐漸向細胞膜聚集。The conclusions further elucidate the damages such as metabolic alteration, membrane damage, enzyme inactivation and genetic alteration may be resulted from the change of glutathione redox system caused by so2. protective roles of seabuckthorn seed oil on oxidative damage induced by so2 were also processed
進一步明確了機體的所有損傷(代謝過程變化、細胞膜損傷、酶失活以及遺傳物質改變)均可能與so _ 2吸入所致谷胱甘肽氧化還原系統發生顯著變化有直接或間接的關系。分享友人