細胞懸液 的英文怎麼說
中文拼音 [xìbāoxuányè]
細胞懸液
英文
cell suspension-
In contrast, n - acetyl - d - galactosamine, sucrose, d - glucose, glycogen, d - mannose, l - fucose and fetuin had no inhibitory effects on hemagglutination. so the lectin activity in the humoral fluids of amphioxus is sh group - dependent and is specifically inhibited by various d - galactosides. also, the humoral fluids obtained from e. coli - injected amphioxus showed increased agglutinating activity against human b and o, rabbit, grass carp and toad erythrocytes, but not against human a and chick erythrocytes, hinting that there might be two types of lectins in amphioxus humoral fluids
條紋斑竹鱉血清對懸浮在egta一mg一gvb緩沖液中的正常的兔血紅細胞、綿羊紅細胞、及部分哺乳動物、鳥類、兩棲類和魚類等代表動物的血紅細胞具有溶血活性;在gvbz十存在時對抗體包被的綿羊紅細胞具有溶血活性,而在edta一gvb緩沖液中不論是對正常的兔紅細胞或其他脊椎動物的紅細胞還是對抗體包被的敏感綿羊紅細胞都沒有溶血活性。In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分試驗中,首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液,然後經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離,通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量,並以伊紅y排斥試驗測定細胞的存活率。After fertilization granulosa cells and oocytes were isolated and continued to be cultured for 48 hours, and made into suspension ; the levels of fsh - r on the the granulose cells shall be immediately measured. results : 1
受精後分離顆粒細胞中文摘要和卵母細胞,將所剝離下的全部顆粒細胞繼續培養48 』 j 』時,製成混懸液,並及時進行顆粒細胞膜上fsh丑含量的測定。The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins, which were existed in the testis tissue protein of mouse, rat and human. 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old. the antiserums and the washings of vaginal membrane were detected by elisa, and shown the highest level of the specific antibody igg was 1 : 6000, while the iga was 1 : 300
二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后,在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ,最高效價分別達到1 : 6000和1 : 300 ;免疫后的小鼠脾臟淋巴細胞增殖率升高,淋巴細胞培養上清液中分泌的il 4和inf y也升高,且以il 4更明顯。Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba
擴張床吸附( eba )技術是一種新型的生化分離技術,它集成了固液分離、濃縮和初期純化於一步單元操作之中,可以直接從含有細胞和細胞碎片的發酵液或培養液中提取目標蛋白,而不必事先除去懸浮的固體顆粒。Studies of the crystal structure of endostatin have shown a compact globular fold, with one face particularly rich in arginine residues acting as a heparin - binding epitope, this site was recently shown to be involved in the inhibition of induced angiogenesis. experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion. recombinant endostatin from bacteria is largely insoluble, but still efficient in arresting tu mor growth after injection into mice. intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors, including lewis lung carcinoma, to a dormant state. no sign of drug induced resistance has been reported and, in the original study, the treatment dormancy appeared to persist even when therapy was discontinued. sowe regard endostatin as a promising anti - tumor drug
許多研究表明重組內皮抑素特異性抑制內皮細胞增殖,而且這種抑制作用呈劑量依賴性。細菌表達產物內皮抑素大部分以不溶形式存在,將這種混懸液注射治療老鼠仍可以抑制腫瘤生長。于小鼠皮下重復注射內皮抑素重組蛋白,幾乎完全抑制鼠lewis肺癌等多種腫瘤生長,並無耐藥性產生,即使中斷治療腫瘤也不再復發。The who classification states that acute lymphocytic leukemia ( all ) should be classified by the pattern of reactivity of cell to a panel of lineage - associated antibodies and, where possible, genetic abnormalities. the expression of cd antigens on leukocytes is currently determined by flow cytimertry, which is expensive and labor - intensive, requiring 5 - 20ul quantities of fluores - cently labeled antibodies and allowing concurrent analysis for a limited number of cd antigens, usually three to four. from clinical point of view, an efficient method is required for analysis of a large number of samples in a single experiment
我們設計了一種新型的細胞晶元,其原理是充分利用玻片的硬度高,透明性好的特點,應用細胞膜表面不同的抗原物質,與包被在玻片上的不同抗體發生特異性結合,通過一次實驗可以將被測細胞懸液中不同膜表型的細胞分離、固定在同一張玻片的不同特定區域,保持了細胞的完整性及活性,又可通過簡單的操作一次將多細胞懸液進行免疫分群。The self - segregation behavior of amphiphilic copolymer on pdl - la scaffold was investigated via fluorescence - labeling technique. the modified scaffold with hydrophilic surface will not only favor the penetration of cell suspension and culture medium, but also provide the microenvironment for the growth of cells with the peo spacer combining amino acid ( rgd ) structure. according the above result, the cytocompatibility test was also performed on pdl - la 3d scaffold modified by amphiphilic copolymer with alkaline amino acid end
這種親水表面不僅有利於細胞懸液和培養介質的進入,並可以通過peo橋聯的氨基酸( rgd )為細胞在三維多孔支架內的生長提供類細胞外基質環境;根據以上結果,本文對堿性氨基酸為peo鏈端基的兩親共聚物-氨基酸類細胞外基質修飾的聚乳酸三維支架進行了細胞相容性的測試。The paper adopted the common breeding way by series of mutagen treating to the yeast ' s cell and spheroplast. finally, the excellent mutant 75l 3555 was obtained. its nature of the production was determined and it can product ethanol on the condition of 40 and have high fermentative speed and stable production. on the other hand, the test was made that the best fermentative condition was found. in the malt juice, adding 0. 02 % k + or 0. 03 % a13 + can increase the production strikingly and adding some degree concentration trahalose can increase the production too and cut short the time of taming. in the study of using molasses producting ethanol, we can obtain high productivity contained the following composition : nitrogen source 1. 5 % maize and 0. 075 % ( nh4 ) 2so4
本實驗以常規的育種方法,通過對酵母細胞懸液和原生質體進行一系列誘變,經過大量的篩選,最終選育出一株耐高溫高產菌株75l _ ( 3566 ) ,並對其進行了生產性能的測定。 75l _ ( 3566 )在40條件下,濃度為20 bx的麥芽汁發酵液中,產酒率高達11 . 5 ,而且發酵速度快,發酵周期可縮短12h ,其生產性能穩定,可以適應大部分以澱粉質為原料的生產廠家的需要。同時本實驗對75l _ ( 3566 )進行了發酵條件優化的初步研究。As we known, there are some peculiar proteins in malignant tumor cells due to the gene mutation
尤其對于象血液、胸腹水等細胞種類很多的細胞懸液,十分有效。As a result, more than 97 % of the testicular cells remained their viability after enzymatic digestion
結果組合酶消化后獲得的睪丸細胞懸液中, 97以上的細胞仍然存活。At the same time, the count of neurons with axons, the length of axons, and the ratio of neurons in visual field under microscope in each group were analyzed
將脊髓神經組織細胞懸液置於不同濃度的神經幹細胞條件培養液中培養。The cell - matrix complex was incubated with dmem and 10 % bovine serum under condition of 37, 5 % co2 - the medium was changed daily. ( 3 ) cell - matrix complex paraffin section was made after 7 days incubation
將細胞懸液接種于戊二醛交聯的膠原一殼聚糖多孔膜,加dmem 10小牛血清培養基後放置37 』 c , 5 co 。C57bl / 6 ( h - 2b background ) mice were subcutaneously immunized with selected peptides in ifa ( incomplete freund ' s adjuvant ) respectively. 8 days later after immunization, spleens were removed to prepare splenocytes suspension
將這些肽片段分別與不完全弗氏佐劑制備成乳濁液,皮下注射免疫c57bl / 6小鼠, 8天後,處死小鼠,取出脾臟制備脾細胞懸液。Most commonly used is the mechanical method in which a small piece of testicular tissue is torn apart using needles and the spermatids are released. while enzymatic digestion of human testicular tissue can result in a mixture of cell types including a whole range of spermatogenic cell types
最常用的是機械方法,即利用細針將活檢獲得的小塊睪丸組織撕開,釋放出精子細胞;也可利用酶將睪丸組織消化成細胞懸液,其中包含所有類型的生精細胞。Larval fish of cynoglossus semilaevis g unther were treated with colchicines to get the karyotype, 12 ~ 50 - day larval fish were selected to be put into the solution of colchicine of different concentration for 5 - 6 hours. the fin, and the other part of the fish were cut off to be treated with low concentration infiltration, solidify, eduction and other procedures. at last, the suspending cells can be gained and treated with air - drying method
選取孵化時間為12 50天的幼魚,在不同濃度的秋水仙素溶液中處理5 6小時,分別剪取冠狀幼鰭、鰓絲和身體周圍的鰭條,通過低滲、固定、解離,得到細胞懸液,熱片法滴片, giemsa染色。Analytical model for the transmembrane voltage induced on a permeabilized cell membrane in suspensions exposed to dc pulse fields
直流脈沖場作用下穿孔懸液細胞跨膜電壓計算模型Abstract : after treated by the lymphocyte separation medium, the erythrocytes and lymphocytes were separated successfully in the kidney cells suspension of rice field eel
摘要運用淋巴細胞分離液處理黃鱔腎臟細胞懸浮液后,成功地分離了其中紅細胞和淋巴細胞。Standard test method for automated analyses of cells - the electrical sensing zone method of enumerating and sizing single cell suspensions
細胞自動分析的標準試驗方法.單細胞懸浮液計數和分級的電敏域法After being cultured three generations and depleted feeder cell layers, the es - d3 cells differentiated into ebs efficiently in the culture media supplemented with homemade green season fbs and - me in petriperm bacterial - culture dish
二、 inbmec cm誘導小鼠es細胞生成造血干祖細胞將獲得的4debs製成單細胞懸液,加入inbmec cm誘導4debs的單細胞生成造血干祖細胞。分享友人