細菌培養物質 的英文怎麼說
中文拼音 [xìjūnpéiyǎngwùzhí]
細菌培養物質
英文
bacterial culture material- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
- 質 : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
- 細菌 : germ; bacterium (pl. bacteria); fungus (pl. fungi)
- 物質 : matter; substance; material
-
It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ) it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中,細菌污染是影響疫苗質量和產量的關鍵性因素,筆者通過了兩年左右的時間,選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份,進行細菌學檢查,並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗,結果表明:我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌,潛在威脅是桿菌和鏈球菌;強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果,可作為疫苗生產后備抗菌手段參考Culture medium a mixture of nutrients used, in liquid form or solidified with agar, to cultivate microorganisms, such as bacteria or fungi, or to support tissue cultures
培養基:以液體或添加瓊脂的固體形式人工配製的適合微生物,如細菌或真菌以及組織培養生長要求的混合營養物質。A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接;用連接產物轉化大腸桿菌jml09感受態細胞,置含amp 、 x - gal和iptg的lb平板上培養12 20小時;挑取白色菌落於選擇性培養基擴大培養,堿裂解法小量提取質粒dna ,並進行酶切分析鑒定,結果獲得整合有prvge基因的重組質粒pugedna ,並與其它prv分離株進行ge基因序列同源性分析。Medical microbiology - culture media - part 3 : dip slides for microbiological urine analysis
醫藥微生物學.細菌培養介質.第3部分:尿樣微生物分析Medical microbiology - culture media - part 4 : transport systems for specimens containing bacteria
醫藥微生物學.細菌培養介質.第4部分:細菌標本運輸系Depending on the chemical added to the bacterial broth, the proteins of one gene would effectively be deactivated, disabling that gene. “ the toggle switch is significant because no further modulation is necessary, ” cantor says
藉由在細菌培養液中添加不同的化合物,將能有效抑制一個基因的蛋白質表現,進而使該基因失效。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態;應用原子力顯微鏡掃描,比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別;用透射電鏡觀察細胞壁完整性,以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變,並通過實驗分析多種物質和因素對潛生體形成的影響。The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明,融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上,融合蛋白主要以不溶性包涵體的形式存在於細胞中,少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。Applied constant keeper can enable the microorganism to continuously culture in double periods of “ constant flowing method speed ”. ? it has a process as such : according to the multiplying speed that the bacteria cultures in double growing periods, and the speed that it consumes the nutritious material, fresh culturing liquid is continuously added. ? at the same time, the same amount of well - cultured bacteria liquid is continuously drawn out, so that the next two items are kept relatively constant : the added material and the consuming material ; the amount that the bacteria have multiplied and that has been drawn out
應用恆化器,能使微生物以對數期「恆流法速度」連續培養,即根據菌種在對數生長期繁殖的速度和消耗營養物質的速度,不斷加入新鮮培養液,同時不斷抽出等量培養好的菌液,使加入物與消耗物、細胞繁殖量與細胞物質抽出量,保持相對穩定均恆。In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg
同時將原核表達載體pet - 28c用nco , xho雙酶切,回收酶切產物,將回收的酶切產物pea , h3 ,載體進行連接,並轉入dh5感受態細胞內,培養12 - 18小時后,挑取陽性菌落,經nco , xho雙酶切分析及pcr檢測,篩選到陽性克隆,其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。分享友人