組成酶原有酶 的英文怎麼說

中文拼音 [zǔchéngyuányǒu]
組成酶原有酶 英文
constitutive enzyme
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 成 : Ⅰ動詞1 （完成; 成功） accomplish; succeed 2 （成為; 變為） become; turn into 3 （成全） help comp...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  • 有 : 有副詞[書面語] （表示整數之外再加零數）: 30 有 5 thirty-five; 10 有 5年 fifteen years
  • 組成 : form; make up; compose; formation; composition; configuration; make-up; compo
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Inhibitors of cyp51, such as azalanstat ( rs - 2i607 ) and rs - 2i745, could inhibit the synthesis of ff - mas to decrease the accumulation of ff - mas. inhibitor of a14 - reductase, such as ay9944 - a - 7, could inhibit the metabolism of ff - mas to increase the accumulation of ff - mas ; and some other reagents, such as nystatin, could combine with the downstream intermediate in the cholesterol biosynthesis pathway to accumulate mas. in this study, we investigated the role of mas by using these reagents to change the level of endogenous ff - mas

    抑制cyp51酶的抑制劑，如azalanstat （ rs - 21607 ）和rs - 21745等，均能抑制mas的合成，降低mas的積累；而抑制14 -還原酶的抑制劑，如ay9944 - a - 7等，能抑制ff - mas向t - mas的轉化而造成ff - mas的積累；還有一些物質，如制黴菌素，能阻止mas向下游的代謝而造成其積累，本文主要通過應用這些物質降低、或增加組織細胞中內源性mas的積累，來研究mas的作用。
  3. There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i

    G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。
  4. Secondly, analysis of peroxidase isoenzyme with polyacrylamidedel electrophoresis for was performed in order to investigate the changes of gene expression under sound stimulation. it could be seen from electrophoresis gel that each group had 6 enzyme bands. new enzyme band in pod electrophoretogram was n ' t detected for stressed groups

    此外，在部分實驗組的培養基中加入不同濃度的蛋白質合成抑制劑環己亞胺酮（ chm ）后發現， pod和cat的活性有所降低，暗示著聲波處理使保護酶活性升高的原因可能是聲波處理促進了細胞內酶的合成。
  5. This paper describes several latest industrial microbial technologies in detail, which are the synthesis of the chiral diols by epoxide hydrolase from microbie, cofactors regeneration for redox with fdh, production of nano / micro wire by the phage display, metabolic network rebuilding for conventional fermentation and the application of the organic solvent tolerance and the metagenomics technology

    本文綜述了幾項最新的工業微生物技術，主要包括：微生物環氧化水解酶催化合成手性二醇、微生物甲酸脫氫酶用於再生氧化還原反應的輔因子、通過噬菌體展示技術得到納米級金屬絲、代謝網路改造和重建用於傳統發酵生產以及有機溶劑耐受菌和宏基因組技術的應用。
  6. Phaenerochaete chrysosporiun produces extracellular peroxidase system mainly consisting of lignin peroxidase and mn - dependent peroxidase, which has peticuliar mechanism of enzyme for degradation of many organic pollutants

    摘要黃孢原毛平革菌由於其所產胞外過氧化物酶系（主要由木素過氧化物酶和錳過氧化物酶組成）的獨特酶降解機理，能降解多種有機污染物，在環境工程中有著巨大的應用前景。
  7. 20 - oxidase expressed very low in expanded leaves of apple, it suggest that the ga intermediates synthesis by the expanded leaves may transport to other vigorous growing tissue as raw material for those tissue

    20 -氧化酶在蘋果成年葉中表達量很低，說明上游合成的赤霉素中間產物有可能外運作為其它旺盛生長組織的ga合成原料。
  8. Contain optimized fiber reconstructing ingredient, the product can make skin firm and rich from within, effectively protect skin fiber caused by collagen enzyme and elastase, promote to reconstruct fiber, improve elasticity and extension of skin, reduce and remove wrinkles from within and improve skin firmness

    含有創新纖維重組成份，能從肌膚內調整出緊致、飽滿的肌膚，有效抵抗膠原蛋白酶和彈性蛋白酶對皮膚深層纖維的破壞，促進纖維組建，從而提高肌膚內部彈性與張力，促使肌膚由內而外減少並平整皺紋，提高肌膚緊致度。
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