組試劑 的英文怎麼說
中文拼音 [zǔshìjì]
組試劑
英文
groureagent-
Chinese title, english title, author, the affiliation and address of the first author, journal title, publication year, volume, issue, page, publication type, check tag, history of medicine, grant type, subject headings, classification code, language, chinese abstract, english abstract, animal variety, dosage forms, pharmacology of chinese herbs, pharmacology, name of disease, diagnosis standard, pathogenesis, trial type, treatment of disease, name of drug and formula, chemical name of medicines, english name of medicines, ingredients and dosage of formula, drug compatibility, usage and dosage, acupuncture and tuina points, acupuncture and tuina manipulation, side effect, therapeutic effect guideline, result of cure, materia medica, chemical structure, physical and chemical properies, effective component, chinese medicine identification, method of processing, pharmaceutical techniques, medicinal action and pharmacological effect, pharmacokinetics, and toxicology
中文文題、英文文題、作者、第一作者單位、第一作者所在地、期刊名稱、出版年、卷、期、頁碼、文獻類型、特徵詞、醫學史、資助類型、主題詞、關鍵詞、分類號、語種、中文文摘、英文文摘、動物品種、劑型、中藥藥理、西藥藥理、疾病名稱、診斷標準、疾病機理、試驗方法、疾病治療、藥名方名、化學藥名、英文藥名、組成劑量、藥物配伍、用法用量、針推穴位、針推方法、不良反應、療效指標、療效結果、藥材學、化學結構、理化性質、有效成分、中藥鑒定、炮製方法、制藥工藝、藥物作用與藥理效用、藥代動力學、毒理學。In the preparation of cell material for staining, the dissolving away of cell contents so that the distribution of tissues may be better observed, using reagents such as sodium hypochlorite
2洗滌:在細胞等生物材料染色的過程中,把細胞的內容物溶去的過程。目的是更好的觀察組織的分佈情況,使用的試劑有次氯酸鈉。Abstract : selcting three groups of solvents as extraction menstrual, the best solvent and the best extraction condition are determined
文摘:選用3組溶劑作為萃取劑,利用正交試驗確定了萃取蕃茄紅色素的最佳溶劑及條件In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Total rna was extracted from the second stage larve of hypoderma sp, then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer. the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software
本試驗的目的旨在進行hypodeminc ( hc )和hypodermina ( ha )基因的克隆、測序、構建重組表達載體並誘導表達,獲得重組抗原,以解決天然抗原的不足並為診斷和免疫試劑的產業化奠定基礎。Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增(長距離pcr擴增和分段pcr擴增)的高純度、大片段(至少為pcr產物長度的4倍)的dna模板,應用三種方法:低熔點瓊脂糖包埋法, sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法,分別提取獲得了枯草桿菌基因組dna ,並對3種方法的操作程序進行了不同程度的改進,結果表明:低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法,採用0 . 5瓊脂糖凝膠電泳3h ,仍然跑不出加樣孔。Since there are more things in the bud tissue of the male flower, such as phenolic compounds, polysacchrides, proteins and some other secondary metabolites, three methods used to isolate rna are tested in this assay, which are the enzyme digesting methods of ctab, ctab - dna and sds - dna
克服了常規方法和試劑盒無法提取出富含酚類化合物、多糖和一些尚無法確定的次級代謝產物的白樺花芽組織rna的障礙,提出了3種白樺雄花芽組織rna的提取方法: ctab 、 ctab - dna酶消化法和sds - dna酶消化法。Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為: ( 1 )照射后12h ,收集nih3t3細胞,用流式細胞儀檢測各組細胞的細胞周期, pcr - sscp檢測抑癌基因p16的變化; ( 2 ) nih3t3細胞照射后立即收集細胞和細胞上清,用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化; ( 3 ) western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 ( mmp - 2 )的表達變化。According to their pathogenic activity, three strain of marine bacteria were selected as l2 ( alteromonas sp ), g ( pseudomonas sp ), py ( pseudomonas sp ). the biochemical changes of malondialdehyde ( mda ), which is the lipid peroxidation end product and can be measured by thiobarbituric acid ( tba ) regeant, and polyphenol content, which reflected the host non - specific chemical defense activity and can be measured by folin - ciocalteu method, were determined at different time intervals during host pathogenesis
本研究採用海帶作為模式藻類生物,以褐藻酸降解菌l2 , g及非褐藻酸降解菌py為復染菌建立人工復染體系,用硫代巴比妥酸( tba )試劑測定抗感染過程中海帶組織細胞膜脂肪氧化產物丙二醛( mda )的變化情況及folin - ciocalteu酚試劑測定病原菌感染過程中海帶組織中多酚含量隨時間變化情況。Nuclear particle track - etched anti - counterfeit marking is a new weapon against fake products. the mark is manufactured by intricate high technology in state - controlled sensitive nuclear facilities which ensures that the mark can not be copied. the pattern of the mark is characterized by its permeability, and can be distinguished from fakes by using a transparent liquid ( e. g. water ), colored pen or chemical reagent. the technique has passed the official health safety examination and poses no danger of nuclear irradiation
用核粒子照射塑料薄膜形成徑跡,再經化學試劑蝕刻和成像技術,得到由微米級微孔組成的圖案.這種圖案具有物質透過特性.用這種方法生產的核徑跡防偽標志,具備核尖端技術不易擴散,製作設備不易得到,產品用其他方法難以偽造,防偽識別簡單、快速、可靠等特點.此種標志已經通過放射性安全檢測,可以用於各種商品(包括食品)的包裝Histological test reagents
組織學檢驗試劑Using processed march cylinder, tapered circular mould, u - shaped instrument, l - shaped concrete fluidity instrument and some other testing instruments, and after research and study to physical and chemical property of various raw material. systematic tests have been carried out in respect of consistency between cement and additive, concrete fluidity, concrete filling - up - space and penetration capability and concrete anti - segregation property, basing on prudent and careful analysis to results of 34 - time tests a nd more than 500 data, and with utilization of combined additive, we finally conclude the proper mix design range for the self - densifying high performance concrete with ideal working performance at all respects
通過加工的march筒、截錐形圓模、 u型儀、 l型混凝土流動儀等實驗儀器以及對各種原材料的物理及化學性能展開詳盡的調查研究,進行了多種材料下的水泥與外加劑相容性、混凝土的流動性、混凝土的填充性和鋼筋通過性、混凝土的抗離析性等系統試驗,在對34組試驗、 500多個數據的詳細認真分析的基礎上,利用復合后的外加劑,得出各項工作性能均較佳的自密實混凝土所用原材料比例范圍。Skin can be observed in its native state in vivo without the fixing, sectioning and staining that is necessary for routine histology
它能在活體組織觀察細胞形態和組織結構,甚至在使用增強對照的試劑后,能在分子水平觀察細胞的活動。2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞,進行瞬時表達, 48小時后,用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。His group began working to develop agents to target hydroxyapatite six years ago, but efforts were stalled by the inability to manufacture large quantities of the agent
他的研究組在6年前就開始尋找這個顯示羥磷灰石的試劑了,但是由於沒有能力大量製造此造影劑而被迫停止。Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k. a 109bp fragment was amplified from the human genome dna. 2. in 166 han samples, 19 ( 11. 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
用紅細胞裂解液和pcr緩沖液非離于去污劑蛋白酶k兩種自配試劑成功地提取了所收標本的人白細胞基因組dna ,並以此為模板成功擴增出預期的109hpmbl目的基因片段。At the same time, 10 serum sample were detected by elisa kit made in abroad and agp. the result of three elisa detection shows : s / p of 8 detected serum higher than 0. 2 is positive ; s / p of 2 detected serum is negative. the result accords with the result of agp detection
最後,再將自製vp1板用自配試劑檢測其活性,同時用自製板和國外試劑、國外elisa試劑盒、瓊脂擴散試驗檢測同樣10份血清作比較,三組elisa檢測結果,其中有8份被檢血清s p (抗體水平)大於0 . 2 ,為陽性; 2份被檢血清為陰性。Through the further experiment, the best proportion of the reagent ingredients and the meat appropriate amount rice sample were obtained
通過進一步試驗,尋找到該檢測試劑各組分的最佳比例以及新鮮度檢測時大米樣品的最佳用量。In general, two forms of combinatorial library synthesis exist, “ liquid - phase ” synthesis and “ solid - phase ” synthesis ; in the latter the chemistries are performed with one or more of the reagents attached to an inert solid support such as a bead or column
總體而言,有兩種形式的組合文庫合成, "液相"合成和"固相"合成,對後者來說,化學反應是與附在支持物(如:玻珠和柱子)上的一種或多種試劑發生的。The results show that these two kinds of fibers are different in shape and physical properties and can be sccuessfully differentiated with zncl - i2, and it is pointed out that this chemical method can be used for the quantitative analysis of the products made out of milk protein fibers
結果表明,兩種不同基體的牛奶蛋白纖維在形態結構特徵和物理機械性能方面均有一定差異;採用氯化鋅碘試劑法可成功區分兩種纖維,該方法也可用於對其產品組分做定量化學分析。分享友人