結構同源性 的英文怎麼說
中文拼音 [jiēgòutóngyuánxìng]
結構同源性
英文
structural homology- 結 : 結動詞(長出果實或種子) bear (fruit); form (seed)
- 構 : Ⅰ動詞1 (構造; 組合) construct; form; compose 2 (結成) fabricate; make up 3 (建造; 架屋) bui...
- 源 : 名詞1. (水流起頭的地方) source (of a river); fountainhead 2. (來源) source; cause 3. (姓氏) a surname
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 結構 : 1 (各組成部分的搭配形式) structure; composition; construction; formation; constitution; fabric;...
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The amino acid sequences of about 100 kinds of spider toxin have been determined so far. their molecular weights were varied from 3 to 12kd except for black widow spider toxins, which were high molecular mass types. there were plenty of cysteine residues in their sequences
至今已有近百種蜘蛛神經毒素的一級結構被測定,除黑寡婦蜘蛛毒素為高分子量毒素外,其他蜘蛛神經毒素的分子量在3 - 12kd之間,不同種屬來源的毒素同源性很小,序列中含有較多的半胱氨酸。The high homology genes are not found in all genes known. but the putative conserved domains of peptidase have been detected. in the domains the homology comparing with callus gallus cathepsin b cdna is 90 %
Ncbi檢索未見同源性高的基因序列,但發現含有典型的肽酶結構域,編碼蛋白與組織蛋白酶b結構域部分一致性高達90 ,其具體功能有待于進一步驗證。Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva. it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells. another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b. belcheri tsingtauense
S004cdna序列編碼一個156個氨基酸殘基組成的蛋白質,與人的泛素蛋白ubf80具有較高的氨基酸序列同源性( 145 / 156同源) ,且該蛋白具有泛素蛋白ubf80所共有的兩個結構域,即由72個氨基酸組成的泛素介導的蛋白降解結構域和由48個氨基酸組成的核糖體蛋白s27a結構域。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eSurprisingly, in both of archaebacteria and chloroplast, ftsz is the main component in the process of division but it is n ' t effective in mitochodria division, instead is a dynamin protein
此外ftsz還是一種400個殘基的保守蛋白, ftsz與真核tubulin在結構、功能和進化上有一定的相似性及同源性,都具gtpase結構域,屬于g蛋白家族。By the analysis of blastn ( nucleitide level ) and blastp ( amino acid level ), some conversed domains in each protein sequence were dete cted, such as pas domain, pac domain and fhue
對這4個片段進行核苷酸序列和蛋白序列的同源性比對,發現它們與一些功能保守的結構域具有同源性。By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells
將得到的序列在genbank和embl數據庫中進行了同源比較,結果顯示:本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異,編碼的氨基酸有3個氨基酸殘基的差異,同源性為98 ,影響蛋白質高級結構的保守二硫鍵為2個。The ir sequence of mxmybl is most homologous with that of atmyb ( 86. 9 % ) and somewhat homologous with that of stmyb 1 ( 77. 0 % ) ; there is very low homology among n - and c - terminal regions outside of the ir regions of all of the mybs ; the protein mxmybl contains a proline - rich region as well as a serine - rich region near the c - terminus, such structure motifs are implicated in transcriptional activation
9 ,與馬鈴薯stmyb的ir序列的同源性達77 0 ,所有這些nnrb蛋白除了瓜區具有較高的同源性外,其c端和n端幾乎沒有同源性。 mwyb蛋白的c一端還含有一個富含脯氨酸區,這樣的結構基序可能具有激活轉錄的功能。The genome structure of tgev, pedv and prcv had high homology and it was difficult to diagnose tgev by traditional means. two studies were carried out to differentiate the tgev from prcv and investigate the epidemiology of tge
Tgev與豬流行性腹瀉病毒( pedv ) 、豬呼吸道冠狀病毒( prcv )在臨床癥狀上很相似,基因組結構上與tgev具有較高的同源性,傳統的方法很難鑒別。In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica
對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna (包括可能與復甦能力有關的cdna )和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明,牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等) 、調節基因的級聯作用( vp3 , mad3樣基因等) 、結構基因產物調節細胞結構(包括細胞質膜)在脫水脅迫中的穩定性等。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 )為材料分離克隆到一個細胞色素p450基因,命名為bccyp86mf5 , cdna全長1854bp ,含1575bp的完整開放閱讀框,編碼524個氨基酸,其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ;堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ;二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域,半胱氨酸亞鐵血紅素配體信號區等, -螺旋和-折疊分別佔47 . 7 、 45 . 0 ;與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ,與擬南芥cyp86c4的達到85 . 9 。4. bioinformatical analysis of the dna dsb repair system in deinococcus radiodurans : compared with e. coli and yeast, a number of similar genes related to dna dsb repair were found by using blast and domain analysis
4 .耐輻射球菌中dna雙鏈斷裂修復網路的生物信息學初探:以大腸桿菌和酵母中雙鏈斷裂修復系統為參照,主要以序列同源性為基礎,結合結構域相似性分析,在耐輻射球菌中找到了很多相似的基因。Based on the feature technology and object - oriented mechanism, we found the manufacture - oriented three levels ( namely part, feature and geometry ) model of mechanical part and the resource model ; secondly, we realize the concurrent design of mechanical part and process through decomposing cycle, and then we constitute the evaluation guidelines on the basis of evaluation strategy of levels, which carry out from two levels ( part and feature ) and four phases ( part rough evaluation, feature rough evaluation, feature detail evaluation and part general evaluation ) ; thirdly, we put forward some evaluation methods according the evaluation guidelines, which include the rule - based process evaluation method, rule - based and fuzzy evaluation - based method associated with resources, case - bas ed and feature - based cost estimation method ; at last, we give a manufacturing - oriented evaluation system of rotating mechanical part, which validate the evaluation guidelines and method of the dissertation useful and practical
以特徵技術為基礎,面向對象的編程機制為手段,建立了面向製造的三層次(即零件層、特徵層、幾何層)零件模型;同時進行了面向製造的資源建模。第二,通過細化循環,採用分層遞階的評價策略,分兩層(零件層和特徵層)四個階段(零件初評價、特徵初評價、特徵詳細評價和零件總體評價)建立可製造性評價指標;第三,以評價指標為基礎,分別提出了基於規則的結構工藝性評價方法、基於規則和模糊綜合評判定性和定量相結合的加工可行性評價方法、以及基於實例和特徵的加工成本估算方法;最後,以solidworks為開發平臺, vb6為編程工具,初步構建了面向製造的回轉類零件可製造性評價系統,它們驗證了本文評價指標和評價方法的實用性和可行性。The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1
本研究根據genbank發表的gpvb株全基因序列,藉助oligo4 . 1軟體設計一對引物,採用pcr技術擴增gpvh1株非結構蛋白ns2基因,並與pmd18 - t載體連接后測序,結果表明:鵝細小病毒h1株ns2基因核苷酸全長1356bp ,編碼451個氨基酸殘基,與gpvb株的ns2基因相比,核苷酸數目相同,有17個堿基、 6個氨基酸的差異;同源性分析表明:二者核苷酸序列同源性為98 . 75 ,推導氨基酸序列同源性為98 . 67 。The orf1 proteins of all four chinese senv isolates had two motifs ( ftl and yxxk ) which were conserved in replication - associated proteins. in all four chinese senv isolates, the putative non - structure orf2 protein had a cav - like region. the putative orf3 protein had a serine - rich tract preceded by a cluster of basic amino acids ( r and k ). database scaning suggested this region had high a percentage of homology with dna topoisomerase i protein of d. melanogaster, and might play an important role in the replication of such single - stranded dna viruses
所有這四個中國分離株,均保留了o即1中與復制有關的保守序列( ftl和yxxk ) :在推測是非結構蛋白的orfz中均含有cav樣保守區w 『 x7一h一x3一c一xl一c一xs一h ; orf3蛋白的堿性氨基酸(精氨酸和賴氨酸)簇后緊接著一個富含絲氨酸的區域,與d . molanogaster的dna拓撲異構酶i有一定的同源性,推測在單鏈dna的復制中起作用。This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv
為了進一步探尋分離株與標準株的異同,又採用rt - pcr方法,擴增獲得分離株f _ o裂解位點附近的基因片段,經測序后與國際上已發表的新城疫病毒的核酸序列進行比較,結果表明其與標準株和疫苗株之間的同源性較低,僅為82 86之間。經系統發育進化樹分析后,判定該分離株為新城疫病毒( ndv )基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析,結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。With the development of economy globalization and information technology, economic form breaks through the three tradition demarcations on industry, and appears the development trend of high osmosis and diversification among industries. the enterprise expands faster and faster, and its scale becomes bigger and bigger, development of modern enterprise begins to appear the characteristics of structural complexity : the composite structure replaces the simple structure, the collectivizing operation becomes the new model of enterprise growth ; multi - units management replaces the single management, business coordination becomes the new source of enterprise growth ; speed economy replaces scale economy, sustainable development becomes the new content of enterprise growth ; cooperation competition replaces absolute competition, and the structural opening becomes the new choice of enterprise growth
隨著信息技術的飛速發展和經濟日趨全球化,經濟形式突破了傳統的三次產業的界限,產業間呈現高滲透、多元化的發展趨勢,企業擴張越來越快,規模也越來越大,現代企業發展中的結構復雜性特徵開始顯現:復合結構取代簡單結構,集團化成為企業成長的新模式;多元經營取代單一經營,業務協同成為企業成長的新源泉;速度經濟取代規模經濟,持續發展成為企業成長的新內容;合作競爭取代完全競爭,結構開放成為企業成長的新選擇。The cadherin repeated domains of bt - r3 protein share high homology with the cadherin domains from african clawed frog ( xenopus laevis ) and e - cadherin ( epithelial ) from mouse ( mus musculus ). so, the 3rd structure of the cadherin repeated domains of bt - r3 protein should be similar to these domains
Bt - r3受體蛋白的鈣粘蛋白結構域分別與非洲爪蟾( xenopuslaevis )的鈣粘蛋白胞外結構域和小鼠( musmusculus )的上皮鈣粘蛋白胞外結構域有很高的同源性。Blastn analysis showed that each of the 10 sequences had no homology with the structural genes or regulatory genes in the anthocyanin biosynthesis. it was suggested that there were some limitations to isolate the specific ast gene by ddrt - pcr
Blastn分析表明,這10個差異表達的cdna片段與數據庫中花青苷生物合成途徑中的結構基因和調節基因序列沒有同源性,表明用ddrt - pcr的方法克隆特定的ast基因有一定的局限性。374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis. a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources
通過dnastar軟體對f基因47 470nt間片段進行同源性分析比較並繪制了遺傳進化樹枝狀結構發生圖,結合334 1672nt間三種限制性內切酶( re : hinf , bsto及rsa )位點的分佈情況,確定了這些分離株的基因分類地位。分享友人