綠熒光桿菌 的英文怎麼說
中文拼音 [lǚyíngguānggǎnjūn]
綠熒光桿菌
英文
bacillus virescens-
2. expression of gfp gene in b. thuringiensis strain green fluorescent protein gene is a very useful report gene which could be detected easily. it can be used in the detection of b. thuringiensis environmental release and study of the insecticidal mechanism
綠色熒光蛋白基因在蘇雲金芽胞桿菌中的表達綠色熒光蛋白基因是一種容易檢測的報告基因,可用於蘇雲金芽胞桿菌制劑施用后的環境監測,殺蟲機理等方面的研究。The promoter - probe vector phn117 in e. coli and phn127 in g " were further constructed by removing promoter while keeping sd sequence from phn115. a teta / tetr bidirectional promoter fragment from pbr322 was respectively cloned into phn117 and phn127 and the resulted colonies were all fluorescent
並經插入pbr322上665bpteta tetr雙向啟動子片段后得到的轉化子均發綠色熒光驗證,實現了wtgfp在大腸桿菌和華癸中生根瘤菌中的組成型表達。Kustaki : ori44, oriw30 and ori2062 were chosen to construct the gfp resolution vector. there were 9 kinds of gfp resolution vector. all of the recombinant plasmids were introduced into crystal negative b. thuringiensis 8mb 171. the fluorescence of the 9 kinds engineered strain can be detected by the fluorescent microscope
將得到的9種gfp解離載體電轉化到蘇雲金芽胞桿菌無晶體突變株bmb171中,熒光顯微鏡鏡檢顯示9株含gfp的蘇雲金芽胞桿菌重組菌在波長為300nm - 510nm的激發光下可發射綠色熒光。A broad - host - range promoter - probe vector phn1006 was further constructed by transferring promoter - probe unit from phn1005 into ptr102
5 .發現一個發較強綠色熒光的大腸桿菌突變株,提取質粒命名為phn122 。分享友人