缺失擴大 的英文怎麼說
中文拼音 [quēshīkuòdà]
缺失擴大
英文
deficiency exaggeration-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。It was a dislike so little just--every imputed fault was so magnified by fancy.
那種討厭是這樣的不公平--每種無中生有的缺點、過失是這樣地為幻想所擴大。Part 3 : the paper set out from big system and coordination view, use the systematic dynamics method to analyse all - sidedly that the city expands the relation with various kinds of ecological factors, and natural factor discern mode of city develop, point out city ecological problems are production by improper use the environment ecological factor during city expand, it cause the problems production, such as the function of natural system disappearance or lose efficiency ; systematic circulation obstruct, material energy transform efficiency being low, and so on
第三部分：從大系統大協調觀出發，運用系統動力學方法全面分析城市擴展與各種生態因子的因果關系，及城市發展的自然因子識別模式，指出城市生態問題的產生是由於城市擴展對環境生態因子的不當使用，導致自然系統功能的缺失或失效，系統循環受阻，物質能量轉化效率低下等問題的產生。Several trends are converging to reduce the grain area, including the loss of irrigation water, desert expansion, the conversion of cropland to nonfarm uses, the shift to higher - value crops, and a decline in double - cropping due to the loss of farm labor in the more prosperous coastal provinces
糧田面積萎縮是多種趨勢匯合共同作用的後果，包括灌溉用水短缺、沙漠擴大、糧田轉為非農用地、改種高價值作物，以及在比較富裕的沿海省份由於農業勞動力缺失致使一年兩作的萎縮。There are many problems in dividend distribution of listed companies in china : an obvious tendency of abusing dividend signal, dividend policy being discontinuous and unstable, a general phenomenon to distribute little and non - distribution. dividend plan is often accompanied by capital stock expansion such as share allocation and capitalization of share premium. list company usually mistakes allocation dividend distribution, highly encloses money under the pretence of it
目前，我國上市公司股利分配存在的問題主要包括：上市公司濫用股利信號，股利政策缺乏連續性和穩定性；上市公司存在不分配或少分配股利現象；股利分配方案往往伴隨著配股，轉增資本等股本擴張行為；誤將配股作為股利分配，以此為幌子大把圈錢；違反同股同權、同股同利的原則，有失公平；股權結構的差異和股本規模的不同形成股利分配的差異，等等。Many streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangement such as large deletion, and high - level dna amplification of those sequences flanking the deletion
許多鏈黴菌表現出高度的遺傳不穩定性，通常為大片段缺失或基因組重排，以及缺失區域兩側序列的高水平dna擴增。The major disadvantage to equity financing is the dilution of your ownership interests and the possible loss of control that may accompany a sharing of ownership with additional investors
權益融資的主要缺點是稀釋了所有者的利益，隨著其他投資者所佔權益份額的擴大企業控制權也可能失去First, the fact that the phenotypic variation in t1 mainly resulted from transformation of exogenous dna rather than ion beam mutagenesis was verified by rapd - pcr amplification to mutants, exogenous dna - transferred plants and ion beam - implanted plants. second, an absent band, marked by sieg - isso, was found in the rapd - pct amplification to t - 6 and its progenies, which meant that the corresponding mutation was hereditary. this mutation was located between exon 1791 - 2691 and exonl - 395 of abc - transporter gene
T - 6和其t2代植株的rapd - pcr擴增結果中均存在缺失條帶s _ （ 168 - 1850 ， ）表明該缺失條帶對應的變異是可以遺傳的，該變異發生在abc鄭州大學博士論文摘要介即sporter基因exon1791一2691和exonl一395之間。To the incomplete information systems, the dissertation suggests a new method to impute the null values. the method is based on the extending model of rough sets and fully considers the consistence of data and dependency of attributes. this method can not only avoid deleting those entities including null values, but also avoid redundant extension of the incomplete information system
對于不完全信息系統，本文利用粗糙集擴展模型，在充分考慮數合肥工業大學博士論文摘要據相容性和屬性依賴性的情況下，對缺失值進行了有效的估算，既防止了刪除含有空值的對象，使系統缺少典型性，又防止出現過多的、擴展的完全系統。As one of the macro - manipulative measures, planning can be understood on two levels, first, as comprehensive blueprinting of national economy and social development ; second, as regulation imposed upon certain important economic activities in the operation of national economy
尤其在我國現階段，由於市場機制不健全，市場的缺陷有可能人為地擴大；在經濟增長時期和體制轉換時期，又容易發生結構失衡和經濟動蕩，這是市場本身難以防範和克服的；對于地區之間的發展不平衡和個人之間的收入分配懸殊等問題，市場也無能為力。According to the promises our nation took to enter wto, our country will continue to enlarge the foreign trade and this will lead to economic growth of our country. however, with the internationalization of manufacture and marketing, phenomena that products with defects produced in a country cause consumers " or users " personal damage or property loss in the importing country or a third country increases day by day. since there are many differences between the laws on product responsibility of different country, which will baffle our to develop foreign trade under wto rules as well as protect chinese consumers from products with defects, we must strengthen the research to the laws of product responsibility of other countries, especially the major nations of wto, and coordinate the relation between the law on product responsibility of our country and those of other countries, and, perfect the laws on product responsibility of our country
按照我國加入wto的承諾，我國將進一步擴大對外貿易，這對我國經濟增長無疑是一種促進，但隨著產品生產和銷售的國際化，一國生產的缺陷產品在進口國甚至第三國導致消費者、使用者人身傷害、財產損失的現象也日漸增多，而各國的產品責任法又千差萬別，這些法律沖突的存在會阻礙我國在wto規則下發展對外貿易，同時也不利於對我國消費者的保護，因此，我們必須加強對世界各國，尤其是wto主要成員國產品責任法律制度的研究，協調我國與各國產品責任法的關系，完善我國產品責任法律制度。分享友人