In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,經37 、 1小時處理的病毒,仍然能夠在貓源細胞fcwf細胞上生長,並且毒力基本保持不變;
耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0經37作用2小時,毒力僅下降一個滴度;
耐熱性試驗中,該病毒在恆定溫度50 ,設定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恆定時間1小時,設定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細胞上完全喪失增殖能力, cpe消失。生物學試驗,
利用實驗室現有條件,選擇不同的細胞系對該病毒進行培養,發現該病毒對貓源細胞fcwf最敏感; mdck細胞次之; f81細胞經多次傳代,亦可出現cpe ;而vero細胞則不敏感。血凝試驗表明,該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
Anaerobic endurance is fundamental to races in which long sprints determine suc ? cess
在長程沖刺情況決定結果的比賽中,無氧
耐力是勝
利的基礎。
Yueqing city huari fire - proof device co., ltd. is located at hong qiao town of yueqing city, south to wenzhou downtown, north to yan dang mountai " state scenic spot ", 104 national freeways and circumlittoral superhighway intersect hongqiao, being apart from wen zhou ' s airport, the train station is about 30 kilometers, the transportation convenience, the information is flourishing
樂清市華日
耐火器材有限公司位於浙江省樂清市虹橋鎮,南臨溫州市區,北靠旅遊勝地雁蕩山, 104國道及沿海高速公路橫貫虹橋,距溫州機場、火車站約30公里,交通便
利,信息發達。
Defection of the rifampin - resistant mycobacterium tuberculosis
結核分支桿菌
耐利福平
耐藥基因的檢測
I am not here for small talk, monsieur connelly
我可不是來這里聊天的康
耐利先生
Joe : london. nelly ' s meeting me at the airport
倫敦.
耐利到機場接我
Observation of treatment outcome of rifapentine on 80 bacteria positive tuberculosis cases resistant to rifampin
指紋分型分析
耐利福平結核分支桿菌的傳播
She ' s in position. no sign of connelly
她已經到了指定位置康
耐利還沒出現
No speeches. nelly made the same speech
別說了.
耐利也是這么說的
The study of the susceptibility of m. tuberculosis to rifampin by micro - well phage replication assay
利福噴汀治療
耐利福平肺結核80例臨床療效觀察
Reliable detection of rifampin - resistance of mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray
檢測
耐利福平結核分枝桿菌寡核苷酸晶元的研製及應用
Michelle minelli, a forecaster with the national weather service in miami, said the storm was expected to get stronger as it entered warmer waters of the gulf of mexico
國家氣象局預報員米歇爾?米
耐利指出,預計颶風在進入墨西哥灣的溫暖水域后將增強威力。
In recent years, the emergence of drug - resistant mycobacterium tuberculosis, especially rifampin - resistant mycobacterium tuberculosis, has brought a bad efect to the chemotherapy result
近年來,結核分枝桿菌
耐藥株的出現特別是
耐利福平菌株的出現,嚴重影響了化療的效果。
The research results further prove that resistance to rifampin in mycobacterium tuberculosis resulted from the rpob gene mutation and pcr - sscp can quickly detect rifampin resistance in mycobacterium tuberculosis clinical isolates
這些研究結果進一步證實,結核分枝桿菌
耐利福平是由於rpob基因突變所致,採用pcr - sscp可快速檢測結核分枝桿菌對
利福平的
耐藥性。
Then, to get more knowledge about the rpob gene mutations and provide some scientific information to study the molecule mechanism of resistance to rifampin in mycobacterium tuberculosis, the rpob gene sequence of mycobacterium tuberculosis clinical isolates from shannxi was analysed by direct sequencing of pcr products
隨后通過直接測序法對陜西區域內結核分枝桿菌臨床分離株rpob基因pcr擴增產物進行序列分析,了解該區域rpob基因突變情況,為研究結核分枝桿菌
耐利福平的分子機制提供參考。
Meanwhile, among 12 rifampin - susceptible isolates, 3 non - rifampin - resistant isolates have mutation relating rifampin - resistance in codon 531, 526 or 511 of rpob gene respectively. it probably resulted from the incorrect result of drug susceptibility testing
同時,在12株利福平敏感株中, 3株耐非利福平菌株分別在rpob基因的531 、 526 、 511位密碼子發生了與耐利福平有關的突變,可能是藥敏結果有誤所致。