聚糖酶 的英文怎麼說
中文拼音 [jùtáng]
聚糖酶
英文
dextranase-
Studies on the xylanase producer aspergillus niger
黑麴黴產木聚糖酶的研究The effect of adding neutral protease, papain and - glucanase on yeast autolysis were studied
摘要研究了外加中性蛋白酶、木瓜蛋白酶和-卜葡聚糖酶對酵母自溶的影響。Xiaoxia wang, baishan fang, juxiang luo, wenjun li, liaoyuan zhang. heterologous expression, purification, and characterization of xylose reductase from candida shehatae
王曉霞，鄭晨娜，王飛飛，方柏山.共表達木聚糖酶和木糖還原酶生產木糖醇的研究Xylanases can hydrolyze xylans into xylooligosaccharides and d - xylose
此菌株能胞外分泌木聚糖酶。This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。Phytohormone influenced on activity of endo - - mannanase during and following rice seed germination
甘露聚糖酶活性的調控The metabolism of these extreme microbes during the production of maotai liquor would further produce multiple enzymes of thermal stability such as amylase, protease, saccharifying enzyme, cellulose, glucase, xylanase, and each kind of dehydrase involved in redox reaction, and dna polyase etc
茅臺酒釀造過程中極端釀酒微生物代謝產生多種熱穩定性的酶，如澱粉酶、蛋白酶、糖化酶、纖維素酶、葡萄糖甘酶、木聚糖酶、參與氧化還原反應的各種脫氮酶、磷酸烯醇丙酮酸激酶及dna聚合酶等。Study on the production of xylanase from streptomyces microflavus
細黃鏈黴菌產木聚糖酶的研究Purification and properties of an endocellulase from the thermophilic fungus chaetomium thermophile
葡聚糖酶的分離純化及特性Application research on kraft wood pulp bleaching with xylanase pretreatment
木聚糖酶在硫酸鹽木漿輔助漂白中的應用研究The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase
尤其是在木聚糖酶譜帶檢測中發現，突變株發酵液中有三種類型的木聚糖酶，而出發菌株中只有兩種類型的木聚糖酶，並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現，突變株中第三種類型的木聚糖酶不僅表達量很大，活力也很高。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。Purification and properties of endo - chitosanase chib from aspergillus sp. cj
326內切殼聚糖酶的分離純化和性質Conclusion a systematic method for preparation of enzyme - mannanse is established, a high productive strain was got after seducing and selecting from nature, confirmed as brachybacterium spa6 research were conducted on medium and culture method of the strain in order to get the suitable cultural condition of fermentation, the experiment result shows the optimium condition is ph7. 0, temperature 36c ; carbon content 2. 5 %, ventilation in abundence, agitation speed 200r / min
結論1 、以從自然界中篩選出的菌株為出發株，經誘變、篩選，得一高產葡甘聚糖酶菌株，初步鑒定為短桿菌屬brachybacteriumspa6 2 、經誘變、三角瓶培養，該菌株的最適培養條件：培養基ph值7 . 0 ，碳源2 . 5 ，振蕩培養， 200r min ，培養溫度36 ，培養48h 。The xylanase activity of the mutant kept stable after 10 generations. after orthogonal designing experiment, the optimum fermentation conditions of a. niger j 506 were obtained, which is as followed : concentration of the major carbon resource 4 %, ratio between bran and corncob 5 : 5, concentration of glucose 0. 1 %, concentration of ammonium oxalate as supplemental nitrogen resource 2. 0 %, the initial ph of liquid medium 5. 0, 100ml / 250ml flask
經過正交試驗設計，得出突變株a . nigerj506產木聚糖酶最佳的工藝條件為：主碳源濃度4 、麩皮與玉米芯的比例為5 ： 5 、輔加碳源葡萄糖的濃度是0 . 1 、輔加氮源草酸銨的濃度是2 . 0 ，培養基初始ph為5 . 0 ， 250ml三角瓶的裝液量為100ml 。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。Purification and characteristics of an endo - beta - 1, 4 - d - glucanase from aspergillus niger
葡聚糖酶的純化和性質Expression of endo - - mannanase gene from trichoderma reesei in pichia pastoris
甘露聚糖酶基因在畢赤酵母中的表達Isolation and identification of endoxylanase from bacillus subtilis
枯草芽孢桿菌內切木聚糖酶的分離純化與鑒定Study on h - bleaching of pretreatment with xylanase
木聚糖酶預處理結合次氯酸鈣漂白的研究分享友人