聚酞胺 的英文怎麼說
中文拼音 [jùàn]
聚酞胺
英文
polyamide-
The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd
4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌，提取初提物中的總蛋白，進行sds一聚丙烯酞胺凝膠電泳，檢測表達的融合蛋白大小越為gokd 。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。A page gel, stained by the petiodic acid - schiff ' s method to reveal glycoproteins, further displayed that the bindng - protein was a glycoprotein belonging to lectin, which contained 17. 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method. peptide mapping was comparable to the reported protein in protein bank. the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase, aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b, glutamyl - trna reductase, histidyl - trna synthetase
連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 「 . skda ，由二個相同的亞基組成，亞基分子量為」 . ikda ，等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白，其中糖含量為17 . 44 % ，蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素We did the same steps for three times, so we could get the extraction using the steps mentioned. laemmli sample buffer was added to the extracted protein, which were then boiled for 5 min. the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane
樣品蛋白經12 % sds一聚丙烯酞胺凝膠電泳分離后，轉印到硝酸纖維素膜上，與第一抗體4孵育過夜，經tbs洗滌后，再與第二抗體室溫孵育2小時， ttbs充分沖洗后，顯色觀察。Molecular weight distribution curve of 1, 1 ' - diaformacylferrocene - o - phenylenediamine polycondensate has triplet and polydispersity is a little large. it should be pointed out that the fe2p3 / 2 peak appears basically near fe2 + characterizing 708ev in xps of the two polycondensates, this means that the ferrocene group is rather stable in the polycondensates
其中， 1 ， l 』一幾甲酞二茂鐵鄰苯二胺縮聚物的分子量分佈曲線呈現三重峰，表明在高轉化率時發生大分子鏈轉移後生成帶支鏈的高分子，其多分散性也較大。2. 4 preparation of yeast telomerase, the activity of telomerase was examined by the method of trap, polyacrylamide gel electrophoresis and silver staining
2 . 4酵母端粒酶的制備，端粒重復擴增法（ trap ） 、聚丙烯酞胺凝膠電泳及硝酸銀染色法檢測端粒酶活性。( 5 ) for e - 20 epoxide resin / 650 polyamide system, good combination properties have been obtained when the bonded magnet was solidified at 90 ~ 110
5 .對于e一20環氧樹脂/ 650聚酞胺體系，固化處理溫度在90一110時，磁體獲得了良好的綜合性能。Combining sio2 / al2o3 ultrafiltration ( uf ) membrane and pva ( pa ) / sio2 - a12o3 nf membrane, the purification and concentration process of erythromycin ( er ) could be achieved more efficiently such as, energy saving, higher recovery rate and potency of the product as the lower temperature condition by using the uf - nf composite system
在對紅黴素的分離提純研究中， 5102 / a1203無機超濾膜一聚乙烯醇（聚酞胺） / s102一a12o3復合納濾膜混合膜分離體系對模擬紅黴素發酵液中的紅黴素具有較好的分離提取效果。The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。Preparation and characterization of polyaniline doped with binuclear cobalt hexasulfonatephthalocyanine
雙核磺化酞菁鈷摻雜聚苯胺膜的制備和表徵Pcr amplification of hla - dqa1 use the extracted genome dna as template, amplify the target sequence. examine the products by page. the bands is clear and the length is 538bp as it shows, which matches the design
結果1 . hla一dqai基因片段的pcr擴增以提取的全基因組dna為模板，行pcr擴增，產物用聚丙烯酞胺凝膠電泳檢測，可見條帶清晰，產物長度538bp ，與設計相符。分享友人