聚體片段 的英文怎麼說
中文拼音 [jùtǐpiānduàn]
聚體片段
英文
segment fraction of polymer-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank
本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。Sixties primers were used for dna amplification and a total of 237 amplification products range from 450bp to 2500bp were generated. a similarity matrix for all pairwise comparisons was calculated using nei and li ' s formula and then transformed to distance matrix. dendrograms were constructed by applying unweighted pair - group arithmetic average ( upgma ) and neighboring - jointing cluster analyses using the phylip software
在第二部分，應用改進的ctab法提取了石蓴屬和滸苔屬各3個種及作為對照的剛毛藻的基因組dna ， 60個引物被用於擴增，共獲得237個片段大小在450 - 2500bp之間的擴增片段，依據nei和li （ 1979 ）的公式計算出樣品成對比較的相似性距陣並換算成遺傳距離，應用phylip軟體包，按照upgma法和n - j法分別構建聚類圖。High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon
將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上，使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。In chapter three, genomic dna were extracted from four individuals from sinotubimorpha porracea and grateloupia filicina, respectively and from g. livida as a control. sixties primers were used for dna amplification and a total of 120 amplification products range from 250bp to 2500bp were generated. distance matrix and dendrograms were obtained as described in last paragraph
在第三部分，應用ctab法提取了管形藻、蜈蚣藻各4個個體及作為對照的舌狀蜈蚣藻的基因組dna ， 60個引物的rapd分析獲得120個片段大小在250 - 2500bp之間的擴增片段，遺傳距離和聚類分析同前。The study of in - situ construction of cytocompatible surface on pdl - la matrix via amphiphile - amino acid ( rgd ) hybrid self - segregation - the amphiphilic diblock copolymer, poly ( dl - lactide ) - poly ( ethylene oxide ) ( pla - peo ) copolymer, containing hydrophobic pla block and hydrophilic peo block was synthesized via coupling method in this dissertation. cell - adhesion - promoting amino acids and integrin receptor peptide rgd were then immobilized at the end of peo chain of pla - peo copolymer via hydroxyl group activation technique. the solvent blending and casting method was then used to obtain the amphiphile modified pdl - la membranes
兩親共聚物-氨基酸（ rgd ）雜化體原位自修飾構建聚乳酸細胞相容性表面的研究一本論文首先設計併合成了一類含疏水聚乳酸（ pla ）鏈段和親水聚氧乙烯（ peo ）鏈段的兩親嵌段共聚物材料（ pla - peo ） ，利用peo鏈端的活性官能團羥基固定了促細胞粘附的氨基酸及整合素配體多肽片段rgd 。Based on the " self - migrating and surface - segregation " behavior, the amphiphile segregation surface was therefore constructed on hydrophobic pdl - la matrix, which containing the structure of peo spacer combining cell adhesion ligands to mimic the extracellular matrix ( ecm )
基於兩親共聚物在疏水高聚物材料內的自遷移和表面富集行為，在聚乳酸材料上構建了以peo橋聯氨基酸或整合素配體多肽片段的類細胞外基質表面。Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science
為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers
將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。The tio2, cds and cds - tio2 films on the common glass substrate were prepared, respectively, using ti ( oc4h9 ) 4, cd ( cooch3 ) 2 and scn2h4 as raw materials by sol - gel method. the influences of manifold preparing parameters ( such as the concentration of sol, the amount of the peg. the number of coatings, the heat - treated temperature and time ) on the structure and performance were studied
本文採用溶膠-凝膠技術，以鈦酸丁酯、乙酸鎘和硫脲為原料，以普通玻璃片為載體，制備了納米tio _ 2薄膜、 cds薄膜和cds - tio _ 2復合半導體薄膜，研究了制備過程中多種制備參數（如溶膠的濃度，聚乙二醇（ peg ）的加入量，鍍膜層數，熱處理溫度及時間）對薄膜結構和性能的影響，採用x -射線衍射（ xrd ） 、掃描電鏡（ sem ） 、能譜分析（ edxa ） 、紫外-可見吸收光譜（ uv - vis ）等測試手段對各薄膜進行了結構和物性表徵。分享友人